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Solarska-Sciuk K.,University of Lodz | Gajewska A.,University of Lodz | Skolimowski J.,University of Lodz | Mitura K.,Institute of Technology and Education | Bartosz G.,University of Lodz
Biotechnology and Applied Biochemistry | Year: 2013

In recent years, the development of nanotechnology opens up new prospects for biomedical applications of unmodified and chemically modified diamond nanoparticles (DNPs). The problem of biocompatibility of DNPs is thus of primary importance. The first step in the modification of DNPs is usually the introduction of OH groups, which can bind other functional groups. One of the basic methods to introduce OH groups onto DNPs is the Fenton reaction. The aim of this study was to compare the effect of unmodified DNPs and nanoparticles modified by the Fenton reaction on human endothelial cells. Ultradisperse diamond (UDD) was modified by the Fenton reaction introducing surface OH groups. Immortalized human umbilical cord endothelial cells (HUVEC-ST) were incubated with 2-100 μg/mL nanopowders in the opti-MEM medium. For comparison, graphite powder (GRAF and GRAF+OH) was also employed. UDD and GRAF augmented generation of reactive oxygen species in the cells after 24 H incubation, estimated by oxidation of 2′,7′-dichlorofluorescin diacetate (H2DCF-DA). Cellular production of nitric oxide, estimated with DAF-FM-DA (3-amino-4-aminomethyl 2′,7′-dichlorofluorescein diacetate), was also affected by UDD and GRAF after 24 H. Fenton-modified OH, in contrast to unmodified diamond, decreased NO production. Detonation nanoparticles also affected the cellular content of glutathione and activities of main antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and glutathione S-transferase). This article was published online on 5 February 2013. Errors in the byline and affiliation line were subsequently identified. This notice is included in the online and print versions to indicate that both have been corrected 18 April 2013. © 2013 International Union of Biochemistry and Molecular Biology, Inc. Source


Giouvanakis T.,University of Macedonia | Giouvanakis T.,Institute of Technology and Education | Fragidis G.,Institute of Technology and Education | Koumpis A.,Research Programmes Division
International Journal of Emerging Technologies in Learning | Year: 2011

In order for a user to develop rich and valid knowledge structures, not only is considerable predefined content required, but other equally important types of knowledge as well. In other words, to display real understanding and to build and extend their knowledge structures, users have to call on several resources as well as representation, retrieval and construction mechanisms. Apart from the specific content, on which traditional educational environments focus on, a user depends heavily on general content knowledge and on systems of knowledge that is more abstract and elusive than typical content. In this article, we document our myCourse approach presenting three application scenarios. In these scenarios, knowledge creation is a process of emergence and co-evolution of the individual, the social group and the wider society. Source


Giouvanakis T.,Institute of Technology and Education | Giouvanakis T.,Research Division | Fragidis G.,Institute of Technology and Education | Ignatiadis I.,Research Division | Koumpis A.,Research Division
International Journal of Emerging Technologies in Learning | Year: 2010

We adopt the direction towards new educational and pedagogic paradigms, where learning is a process of emergence and co-evolution of the individual, the social group and the wider society. In this direction, Service-Oriented Architectures are becoming a popular system paradigm for e-learning. In this article, we present our research and development efforts to provide a social networking learning platform for developing services which address the personal learning needs of the users and enable them to create value. We also present the specific characteristics of our community driven service framework and discuss our approach in comparison to other similar approaches and frameworks. Source

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