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Huang X.,Hubei University | Huang X.,Institute of Stomatological Research | Yang T.,Hubei University | Zhao S.,Wuhan University of Technology | And 2 more authors.
Journal Wuhan University of Technology, Materials Science Edition | Year: 2012

The effect of glass ionomer cement and resin-modified glass ionomer cement incorporated with chlorhexidine and bioactive glass on antimicrobial activity and physicochemical properties were investigated. The experimental results showed that groups incorporated with 1% chlorhexidine exhibited a significant reduction of optical density values of the bacterial suspension and increased the degradation of Streptococcus mutans biofilm. However, groups incorporated with 10% bioactive glass did not affect the optical density values and the biofilm formation. The mechanical properties of the materials and the polymerization were not influenced by the addition of chlorhexidine. Nevertheless, the compressive strength was lower when the materials were incorporated with bioactive glass. It can be concluded that glass ionomer cements incorporated with chlorhexidine can maintain its mechanical properties as well as reduce early S mutans biofilm formation. Controlled release/sustained release technology may be required to optimize the antibacterial activity of glass ionomer cements incorporated with bioactive glass. © Wuhan University of Technology and Springer Verlag Berlin Heidelberg 2012.


Zhang R.,Cancer Center | Qin W.,Cancer Center | Qin W.,Institute of Stomatological Research | Xu G.-L.,Cancer Center | And 2 more authors.
Colorectal Disease | Year: 2012

Aim The study aimed to understand better the somatic mutations in the human MutL Homolog 1 (hMLH1) and human MutS Homolog 2 (hMSH2) genes in colorectal cancer (CRC) and to investigate the differences derived from ethnicity, family history, detection method and microsatellite instability (MSI). Method The terms 'hMSH2' or 'hMLH1'and'colorectal cancer''colorectal carcinoma' or 'colorectal tumour' were searched in the PubMed, Springer, Lippincott, Williams & Wilkins and HighWire Press databases for the publication period December 1993 to September 2010. The Comprehensive Meta Analysis V2 software (Biostat Inc.) was used to explore the prevalence and 95% confidence intervals. Results The prevalence of somatic mutations in the hMLH1 and hMSH2 genes in CRC was 0.15 (95% CI 0.10-0.22) and 0.10 (95% CI 0.07-0.16), respectively. A higher prevalence of somatic mutations in hMSH2 was found in hereditary non-polyposis CRC than in sporadic CRC: 0.36 (95% CI 0.14-0.67) and 0.10 (95% CI 0.07-0.13) respectively. In addition, a higher prevalence of somatic mutations in the hMLH1 gene was observed relative to hMSH2 in the European group. The prevalence was higher in the high-level instability (MSI-H) group than in both the low-level instability (MSI-L) and the microsatellite stable (MSS) groups. Conclusion Somatic mutations in the hMLH1 and hMSH2 genes play a vital role in CRC and a high prevalence was found in this meta-analysis. Furthermore, more studies are needed which focus on somatic mutations in the American population and in patients with MSI-L and MSS. © 2011 The Authors. Colorectal Disease © 2011 The Association of Coloproctology of Great Britain and Ireland.


Zhao K.,Institute of Stomatological Research | Tian T.,Institute of Stomatological Research | Zhu W.,Institute of Stomatological Research | Yu S.,Guangzhou Yuexiu District Hospital of Stomatology
Journal Wuhan University of Technology, Materials Science Edition | Year: 2011

To develop a new kind of denture adhesive (DA), then to evaluate its mechanical and chemical properties, polyethylene oxide, methyl cellulose, sodium alginate, and cellulose ether, were viewed as four main factors, which would affect bonding load of DA, while two levels of each component were differentiated. Following table L 12(2 11), twelve different formulae were designed by orthogonal design and bonding load values were recorded. According to bonding load of each formula, the optimized formula was selected out as this new adhesive material, Comfort-DA II. The changing of bonding load values of Comfort-DA II marinating in artificial saliva were measured, and an existing product, Protefix and Comfort-DA, were used as controls. Following initial load readings, samples and controls were measured from 1- to 12- hour intervals, respectively. Comfort-DA II was diluted at 1.0%, 2.0%, 3.3%, 5.0%, and 10.0% concentrations, and pH values were measured at 1-, 2-, 3-, 4-, 5-, 6-, and 8-hour intervals. Comfort-DA II presented as a pale-yellow paste. Group 6 gained the highest bonding load value of [193.8 (4.2)] N. The mean bonding load of Comfort-DA II was statistically different to Comfort-DA (p = 0.004) and Protefix (p = 0.006). Comfort-DA II exhibited a progressive increase in pH value over time and was slightly alkaline. Comfort-DA II showed significantly elevated mechanical and chemical properties, comparing to Comfort-DA. The results also indicate that orthogonal design may be an efficacious way to develop new dental materials. © Wuhan University of Technology and Springer Verlag Berlin Heidelberg 2011.


Teng W.,Institute of Stomatological Research | Wang Q.,Sun Yat Sen University | Chen Y.,Sun Yat Sen University | Huang H.,Institute of Stomatological Research
International Journal of Nanomedicine | Year: 2014

To explore a spatiotemporally controllable gene delivery system with high efficiency and safety, polyelectrolyte multilayer (PEM) films were constructed on titanium or quartz substrates via layer-by-layer self-assembly technique by using plasmid deoxyribonucleic acid-loaded lipopolysaccharide-amine nanopolymersomes (pNPs) as polycations and hyaluronic acid (HA) as polyanions. pNPs were chosen because they have high transfection efficiency (>95%) in mesenchymal stem cells (MSCs) and induce significant angiogenesis in zebrafish in conventional bolus transfection. The assembly process of PEM films was confirmed by analyses of quartz crystal microbalance with dissipation, X-ray photoelectron spectroscopy, infrared, contact angle, and zeta potential along with atomic force microscopy observation. Quartz crystal microbalance with dissipation analysis reveals that this film grows in an exponential mode, pNPs are the main contributor to the film mass, and the film mass can be modulated in a relatively wide range (1.0-29 μg/cm2) by adjusting the deposition layer number. Atomic force microscopy observation shows that the assembly leads to the formation of a patterned film with three-dimensional tree-like nanostructure, where the branches are composed of beaded chains (pNP beads are strung on HA molecular chains), and the incorporated pNPs keep structure intact. In vitro release experiment shows that plasmid deoxyribonucleic acid can be gradually released from films over 14 days, and the released plasmid deoxyribonucleic acid exists in a complex form. In vitro cell experiments demonstrate that PEM films can enhance the adhesion and proliferation of MSCs and efficiently transfect MSCs in situ in vitro for at least 4 days. Our results suggest that a (pNPs/HA)n system can mediate efficient transfection in stem cells in a spatially and temporally controllable pattern, highlighting its huge potential in local gene therapy. © 2014 Teng et al.

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