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Patel M.,University College London | Mulhall H.,University of Surrey | Al-Quatani K.,Institute of Sport and Physical Activity Research and UCL Eastman Dental Institute | Lewis M.,Institute of Sport and Physical Activity Research and UCL Eastman Dental Institute | And 2 more authors.
Biotechnology Letters | Year: 2011

Capillary shear stress can improve osteogenic differentiation in muscle-derived precursor cells (MDPCs). This has implications for large-scale bioprocessing of cell therapies where capillary transfer is needed. The recovery, viability, and osteogenic differentiation potential of two subsets of MDPCs, early-adherent pre-plate 1 (PP1) and late-adherent PP3 populations, have been examined: PP1 MDPCs produced a greater degree of osteogenic differentiation than PP3 MDPCs, quantified by Alizarin Red S staining intensity (P < 0.05). For both cell populations, capillary flow-induced significant increases in Alizarin Red S staining (P < 0.05). However, PP1 cells were more susceptible to capillary flow-induced damage than PP3 cells and this was dependent on duration of exposure. Overall, results indicate that different cell subsets, even from within a single tissue, can respond variably to capillary shear stress, necessitating its precise monitoring and control. © 2011 Springer Science+Business Media B.V.


Mulhall H.,University of Surrey | Mulhall H.,Institute of Sport and Physical Activity Research and UCL Eastman Dental Institute | Patel M.,University College London | Alqahtani K.,Institute of Sport and Physical Activity Research and UCL Eastman Dental Institute | And 4 more authors.
Journal of Tissue Engineering and Regenerative Medicine | Year: 2011

Both chemical and physical stimuli can influence the fate of precursor cell populations. Therefore, the impact they have on promoting unwanted differentiation events must be understood to improve the yield and purity of therapeutic cells for regenerative medicine approaches. Capillary shear forces, similar to those encountered during cell processing, can impact upon production of regenerative cell populations. As shear stress can promote osteogenic differentiation in adhered bone marrow-derived stromal cells, we sought to determine whether the same is true for populations of muscle-derived precursor cells (MDPCs) that were isolated from a muscle niche environment. We isolated MDPCs from craniofacial muscle of 5 day-old Royal College of Surgeons rats and subjected them to capillary shear events similar to those encountered during manual bioprocessing of cells. We then assessed whether viability and ectopic osteogenic differentiation of MDPCs was affected. We found that whilst immediate recovery of MDPCs was not significantly affected by shear, viability after 24 h was reduced in comparison to non-sheared MDPCs. By 48 h, sheared MDPCs had all recovered and had similar viability to non-sheared MDPCs. Ostegenic differentiation was enhanced following exposure to capillary shear in both osteogenic and myogenic medium. This indicates that shear forces similar to those encountered during the bioprocessing of cell populations for therapy can have a significant influence on the fate of MDPCs. © 2010 John Wiley & Sons, Ltd.

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