Institute of Special Wild Economic Animals and Plants

Jilin, China

Institute of Special Wild Economic Animals and Plants

Jilin, China
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Han X.-Y.,Jilin Agricultural University | Hu J.-N.,Jilin Agricultural University | Wang Z.,Jilin Agricultural University | Wei S.-N.,Jilin University | And 3 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2017

The aim of this study was to investigate the effects of 5-hydroxymethyl-2-furfural (5-HMF) on liver fibrosis induced by carbon tetrachloride (CCl4) and alcohol. Male ICR mice were treated with CCl4 dissolved in olive oil (10% v/v, 2.5 μg/L) intraperitoneally (i.p.), and given at a dose of 2.5×10−5 mg/kg B.W. twice a week for 7 wk. Concurrently, mice received drinking water with or without alcohol. The mice in treatment groups and positive control group were gavaged with 5-HMF (7.5, 15, and 30 mg/kg B.W.) or Huganpian (350 mg/kg B.W.) daily starting in the fourth week and lasting for 4 wk. The blood samples were analyzed for biochemical markers of hepatic injury and tissue samples were subjected for estimation of liver antioxidants and histopathological studies. The concentrations of HA (hyaluronic acid), LN (laminin), CIV (collagen type IV), and MDA (malondialdehyde), as well as the serum levels of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced by 5-HMF. On the other hand, enzymatic antioxidants SOD (superoxide dismutase), CAT (catalase) and GSH-Px (glutathione peroxidase) were markedly elevated in liver tissue treated with 5-HMF. Histopathological examination revealed that 5-HMF treatment noticeably prevented hepatocyte apoptosis, fatty degeneration and inflammatory cell infiltration on liver fibrosis induced by CCl4 and alcohol. Hoechst 33258 staining also revealed hepatocyte apoptosis. 5-HMF could exert protective effects against liver injury and reduce liver fibrosis induced by CCl4 and alcohol in mice. © 2017, Center for Academic Publications Japan. All rights reserved.


Xu X.-Y.,Jilin Agricultural University | Hu J.-N.,Jilin Agricultural University | Liu Z.,Jilin Agricultural University | Zhang R.,Institute of Special Wild Economic Animals and Plants | And 5 more authors.
Journal of Agricultural and Food Chemistry | Year: 2017

Acetaminophen (APAP) overdose is one of the most common inducements of drug-induced liver injury (DILI) in the world. The main purpose of this paper was to investigate the liver protection activity of saponins (ginsenosides) from the leaves of Panax quinquefolius (PQS) against APAP-induced hepatotoxicity, and the involved mechanisms were demonstrated for the first time. Mice were pretreated with PQS (150 and 300 mg/kg) by oral gavage for 7 days before being treated with 250 mg/kg APAP. Severe liver injury was exerted at 24 h post-APAP, and hepatotoxicity was assessed. Our results showed that pretreatment with PQS significantly decreased the serum alanine aminotransferase (ALT), aspartate transaminase (AST), tumor necrosis factor (TNF-α), and interleukin-1β (IL-1β) levels in a dose-dependent manner as compared to the APAP administration. Meanwhile, compared with that in the APAP group, PQS decreased hepatic malondialdehyde (MDA) contents and 4-hydroxynonenal (4-HNE) expression and restored reduced glutathione (GSH) content and superoxide dismutase (SOD) activity in livers of mice. PQS inhibited the overexpression of pro-inflammatory factors cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) in the liver tissues. Furthermore, Western blotting analysis revealed that PQS pretreatment inhibited the activation of apoptotic signaling pathways via increase of Bcl-2 and decrease of Bax and caspase-3 protein expression levels. Liver histopathological observation provided further evidence that PQS pretreatment significantly inhibited APAP-induced hepatocyte necrosis, inflammatory cell infiltration, and congestion. Biological indicators of nitrative stress such as 3-nitrotyrosine (3-NT) were inhibited after PQS pretreatment, compared to the APAP group. The present study clearly demonstrates that PQS exerts a protective effect against APAP-induced hepatic injury because of its antioxidant, anti-apoptotic, and anti-inflammatory activities. The findings from the present investigation show that PQS might be a promising candidate treatment agent against drug-induced ALI. © 2017 American Chemical Society.


Wei M.,Institute of Special Wild Economic Animals and Plants | Chu W.,Institute of Special Wild Economic Animals and Plants | Zhao H.,Institute of Special Wild Economic Animals and Plants | Wang D.,Institute of Special Wild Economic Animals and Plants | And 2 more authors.
Acta Theriologica Sinica | Year: 2012

In this study, a number of sequences of small interfering RNA targeting S100A4 gene of sika deer (Cervus nippon) were synthesized and screened, among which two were confirmed by BLAST search to be suitable for the purpose. Oligo DNAs containing either sense or antisense strands were ligated into the lentiviral plasmids (pLVTHM) using T4 DNA ligase. Positive clones were identified based on the results of both PCR and sequencing. Each positive plasmid was co-transfected into 293T cells with the plasmids pCMV-dr 8.91 and pMD2. G. Twenty-four hours after the co-transfection, green fluorescence of the 293 T cells could be observed under the inverted microscope. Therefore, we successfully constructed recombinant lentiviral system targeting sika deer S100A4 gene. This work would lay the foundation for revealing the regulatory mechanism of S100A4 underlying antler generation and regeneration.

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