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Han X.-Y.,Jilin Agricultural University | Hu J.-N.,Jilin Agricultural University | Wang Z.,Jilin Agricultural University | Wei S.-N.,Jilin University | And 3 more authors.
Journal of Nutritional Science and Vitaminology | Year: 2017

The aim of this study was to investigate the effects of 5-hydroxymethyl-2-furfural (5-HMF) on liver fibrosis induced by carbon tetrachloride (CCl4) and alcohol. Male ICR mice were treated with CCl4 dissolved in olive oil (10% v/v, 2.5 μg/L) intraperitoneally (i.p.), and given at a dose of 2.5×10−5 mg/kg B.W. twice a week for 7 wk. Concurrently, mice received drinking water with or without alcohol. The mice in treatment groups and positive control group were gavaged with 5-HMF (7.5, 15, and 30 mg/kg B.W.) or Huganpian (350 mg/kg B.W.) daily starting in the fourth week and lasting for 4 wk. The blood samples were analyzed for biochemical markers of hepatic injury and tissue samples were subjected for estimation of liver antioxidants and histopathological studies. The concentrations of HA (hyaluronic acid), LN (laminin), CIV (collagen type IV), and MDA (malondialdehyde), as well as the serum levels of ALT (alanine aminotransferase) and AST (aspartate aminotransferase) were markedly reduced by 5-HMF. On the other hand, enzymatic antioxidants SOD (superoxide dismutase), CAT (catalase) and GSH-Px (glutathione peroxidase) were markedly elevated in liver tissue treated with 5-HMF. Histopathological examination revealed that 5-HMF treatment noticeably prevented hepatocyte apoptosis, fatty degeneration and inflammatory cell infiltration on liver fibrosis induced by CCl4 and alcohol. Hoechst 33258 staining also revealed hepatocyte apoptosis. 5-HMF could exert protective effects against liver injury and reduce liver fibrosis induced by CCl4 and alcohol in mice. © 2017, Center for Academic Publications Japan. All rights reserved.

Wei M.,Institute of Special Wild Economic Animals and Plants | Chu W.,Institute of Special Wild Economic Animals and Plants | Zhao H.,Institute of Special Wild Economic Animals and Plants | Wang D.,Institute of Special Wild Economic Animals and Plants | And 2 more authors.
Acta Theriologica Sinica | Year: 2012

In this study, a number of sequences of small interfering RNA targeting S100A4 gene of sika deer (Cervus nippon) were synthesized and screened, among which two were confirmed by BLAST search to be suitable for the purpose. Oligo DNAs containing either sense or antisense strands were ligated into the lentiviral plasmids (pLVTHM) using T4 DNA ligase. Positive clones were identified based on the results of both PCR and sequencing. Each positive plasmid was co-transfected into 293T cells with the plasmids pCMV-dr 8.91 and pMD2. G. Twenty-four hours after the co-transfection, green fluorescence of the 293 T cells could be observed under the inverted microscope. Therefore, we successfully constructed recombinant lentiviral system targeting sika deer S100A4 gene. This work would lay the foundation for revealing the regulatory mechanism of S100A4 underlying antler generation and regeneration.

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