Institute of Special Wild Economic Animals and Plant

Jilin, China

Institute of Special Wild Economic Animals and Plant

Jilin, China

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Li W.,Jilin Agricultural University | Zhang W.,Jilin Agricultural University | Xiang L.,Shandong University | Wang Z.,Jilin Agricultural University | And 4 more authors.
Molecules | Year: 2010

A new oleanane-type triterpenoid saponin, named platycoside N (1), together with six known saponins, was isolated from the roots of Platycodon grandiflorum. On the basis of acid hydrolysis, comprehensive spectroscopic data analyses and comparison with the spectral data of the known compounds, its structure was elucidated as 3-O-β-Dglucopyranosyl-( 1→6)-β-D- glucopyranosyl-2β,3β,16α,23-tetrahydroxyolean-12-en-28-oic acid 28-O-β-L-rhamnopyranosyl-(1→2)-α-L-arabinopyranoside. The six known compounds were platycodin D (2), deapioplatycodin D (3), platycodin D 3 (4), deapioplatycodin D3 (5), platycoside E (6) and deapioplatycoside E (7). © 2010 by the authors; licensee MDPI, Basel, Switzerland.


Li W.,Jilin Agricultural University | Yan M.-H.,Jilin Agricultural University | Liu Y.,Jilin Agricultural University | Liu Z.,Jilin Agricultural University | And 5 more authors.
Nutrients | Year: 2016

Although cisplatin is an effective anti-cancer agent that is widely used for treating various types of malignant solid tumors, the nephrotoxicity induced by cisplatin severely limits its clinical application. The present study was designed to explore the potential protective effect of ginsenoside Rg5, a rare ginsenoside generated during steaming ginseng, on cisplatin-induced nephrotoxicity in a mouse experimental model. The possible mechanisms underlying this nephroprotective effect were also investigated for the first time. Rg5 was given at doses of 10 and 20 mg/kg for 10 consecutive days. On Day 7, a single nephrotoxic dose of cisplatin (25 mg/kg) was injected to mice. Cisplatin administration resulted in renal dysfunction as evidenced by increase in serum creatinine (CRE) and blood urea nitrogen (BUN) levels. In addition, cisplatin increased the level of malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), the makers of lipid peroxidation, and depleted glutathione (GSH) content and superoxide dismutase (SOD) activity in renal tissues. These effects were associated with the significantly increased levels of cytochrome P450 E1 (CYP2E1), 4-hydroxynonenal (4-HNE), tumor necrosis factor (TNF)-α, interleukin (IL)-1β, nuclear factor-kappa B (NF-κB) p65, and cyclooxygenase-2 (COX-2) in renal tissues. However, pretreatment with ginsenoside Rg5 significantly attenuated the renal dysfunction, oxidative stress and inflammation response induced by cisplatin. Furthermore, ginsenoside Rg5 supplementation inhibited activation of apoptotic pathways through increasing Bcl-2 and decreasing Bax expression levels. Histopathological examination further confirmed the nephroprotective effect of Rg5. Collectively, these results clearly suggest that Rg5-mediated alleviation of cisplatin-induced nephrotoxicity may be related to its anti-oxidant, anti-apoptotic and anti-inflammatory effects. © 2016 by the authors; licensee MDPI, Basel, Switzerland.


Li W.,Jilin Agricultural University | Zhang M.,Jilin University | Wang Z.,Jilin Agricultural University | Wang Y.P.,Institute of Special Wild Economic Animals and Plant | And 2 more authors.
Chromatographia | Year: 2011

Platycosides, main pharmacological effective compounds, are known to have several biological activities, including anti-obesity, anti-cancer and anti-diabetes. Although enzymatic preparation of platycosides was considered as effective method to obtain them, few analytical methods have been reported on process control. In the present study, we developed an application of reversed-phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of six platycosides during the process of enzymatic preparation of deapio-platycodin D (dPD) and platycodin D (PD). The method employed a Hypersil ODS2 analytical column (250 × 4.6 mm I.D., 5 μm) coupled with UV detector at 210 nm with flow rate of 1.0 mg mL-1. A step gradient of acetonitrile-water (v/v) was applied, leading to a sample analysis of 60 min. The method was validated in terms of linearity, sensitivity, precision and accuracy. The correlation coefficients (R 2) for calibration curves of platycosides were in the range of 0.9995-1.0 when the linearity range was from 0.85 to 10.2 mg mL-1. The proposed RP-HPLC method was successfully applied to the analysis of enzymatic preparation study and the recoveries of platycosides were in the range of 96.22-102.56% with RSD <3.3%. The method could be of use for rapid and routine evaluation of the quantity of platycosides during the enzymatic preparation process. © 2011 Springer-Verlag.


Li W.,Jilin University | Li W.,Jilin Agricultural University | Zhang M.,Jilin University | Zheng Y.-N.,Jilin Agricultural University | And 7 more authors.
Molecules | Year: 2011

To investigate the protective effects of protopanaxadiol-type ginsenoside (PDG) and its metabolite ginsenoside M1 (G-M1) on carbon tetrachloride (CCl 4)-induced chronic liver injury in ICR mice, we carried out conversion of protopanaxadiol-type ginsenosides to ginsenoside M1 using snailase. The optimum time for the conversion was 24 h at a constant pH of 4.5 and an optimum temperature of 50 °C. The transformation products were identified by high-performance liquid chromatography and electrospray ion-mass spectrometry. Subsequently, most of PDG was decomposed and converted into G-M1 by 24 h post-reaction. During the study on hepatoprotective in a mice model of chronic liver injury, PDG or G-M1 supplement significantly ameliorated the CCl 4-induced liver lesions, lowered the serum levels of select hepatic enzyme markers (alanine aminotransferase, ALT, and aspartate aminotransferase, AST) and malondialdehyde and increased the activity of superoxide dismutase in liver. Histopathology of the liver tissues showed that PDG and G-M1 attenuated the hepatocellular necrosis and led to reduction of inflammatory cell infiltration. Therefore, the results of this study show that PDG and G-M1 can be proposed to protect the liver against CCl 4-induced oxidative injury in mice, and the hepatoprotective effect might be attributed to amelioration of oxidative stress. © 2011 by the authors; licensee MDPI, Basel, Switzerland.


Li W.,Jilin Agricultural University | Su X.-M.,Jilin Agricultural University | Han Y.,Jilin Agricultural University | Xu Q.,Jilin Agricultural University | And 3 more authors.
RSC Advances | Year: 2015

The purpose of this study was to investigate the anti-hepatoma activity of maltol, a Maillard reaction product, in H22 tumor-bearing mice. The results demonstrate that maltol not only significantly inhibited the growth of hepatoma H22 transplanted in mice, but also prolonged the survival time of H22-bearing mice. Furthermore, the levels of serum cytokines in H22 tumor-bearing mice, such as interferon gamma (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-2 (IL-2), were enhanced by maltol treatment. Importantly, immunohistochemical and western blotting analysis clearly show that maltol treatment increased Bax and decreased Bcl-2 protein expression levels of H22 tumor tissues in a dose-dependent manner. Collectively, our findings in the present study clearly demonstrate that the maltol markedly suppressed the tumor growth of H22 transplanted tumors in vivo at least partly via improving the immune functions, inducing apoptosis, and inhibiting angiogenesis. © The Royal Society of Chemistry.


Li W.,Jilin Agricultural University | Wang Z.,Jilin Agricultural University | Wang Y.-P.,Institute of Special Wild Economic Animals and Plant | Jiang C.,Jilin Agricultural University | And 3 more authors.
Food Chemistry | Year: 2012

Pressurised liquid extraction (PLE) of three major flavones (hesperidin, nobiletin, and tangeretin) from the peels of Citrus reticulata 'Chachi' (Guangchenpi) was investigated. These flavones were quantified and analysed by liquid chromatography-diode array detector-electrospray ionisation mass spectrometry (LC-DAD-ESI/MS). The PLE procedure was optimised, validated and compared with other conventional extraction techniques. PLE gained the best result due to the highest extraction efficiency within the shortest extraction time. The optimal conditions of PLE were employing 70% methanol as extraction solvent at a temperature of 160 °C and extraction pressure of 1500 psi, using one extraction cycle with a static extraction time of 20 min. MS coupling with an ESI interface in the positive ion mode was used as the detection technique. This is the first report on combining PLE with LC-DAD-ESI/MS for the extraction and quantification of flavones in Citrus reticulata 'Chachi'. © 2011 Elsevier Ltd. All rights reserved.

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