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Wang C.,Jilin Agricultural University | Wang Y.,Jilin Agricultural University | Gao J.,Jilin Agricultural University | Xu Y.,Jilin Agricultural University | Cui L.,Institute of Special Wild Economic Animal and Plant Science
Environmental Monitoring and Assessment | Year: 2014

Two-year field trials were performed at two experimental sites to investigate dissipation and terminal residues of propamocarb in ginseng root, stem, leaf, and soil by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Mean recoveries ranged from 80.5 to 95.6 % with relative standard deviations (RSDs) of 5.5-9.1 % at fortified levels of 0.01, 0.02, 0.05 and 0.20 mg kg-1. The half-lives of propamocarb were 5.00-11.36 days in root, 5.07-11.46 days in stem, 6.83-11.31 days in leaf and 6.44-8.43 days in soil. The terminal residues of propamocarb were below the maximum residue limits (MRLs) of EU (0.20 mg kg-1) and South Korea (0.50 mg kg-1 in fresh ginseng and 1.0 mg kg-1 in dried ginseng) over 28 days after last spraying at recommended dosage. The results provide a quantitative basis for establishing the MRL and give a suggestion of safe and reasonable use of propamocarb in ginseng. © 2014 Springer International Publishing. Source


Wang Y.,Jilin Agricultural University | Liu C.,Jilin Agricultural University | Gao J.,Jilin Agricultural University | Wang C.,Institute of Special Wild Economic Animal and Plant Science | And 2 more authors.
Environmental Monitoring and Assessment | Year: 2014

The dissipation and terminal residues of a fungicide suspension (5 % hexaconazole, 25 % kresoxim-methyl) in ginseng and soil were investigated by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). At fortified levels of 0.01, 0.02, and 0.20 mg kg−1, the recoveries of hexaconazole and kresoxim-methyl were in the range of 80.6∼94.8 % and 82.4∼98.8 % with relative standard deviation of 3.42–9.12 % and 3.19–8.58 %, respectively. The half-lives were 7.09–10.73 days in root, 6.80–7.95 days in stem, 5.31–8.49 days in leaf, and 6.30–7.97 days in soil. The terminal residues were all below the maximum residue limits (MRLs) of EU and South Korea. Risk assessment results indicated that the risk of hexaconazole and kresoxim-methyl use in ginseng at dosage of 60–90 g a.i. ha−1 was negligible to humans. This work would help the government to establish the MRL and provide guidance on the proper and safe use of hexaconazole and kresoxim-methyl in ginseng. © 2014, Springer International Publishing Switzerland. Source


Sun L.,Beihua University | Lei X.,Beihua University | Lei X.,Institute of Special Wild Economic Animal and Plant Science | Ma R.,Beihua University | And 5 more authors.
African Journal of Biotechnology | Year: 2011

Effective identification of traditional medicine is essential for the development of medical industry. Identification in different parts (rhizome head, lateral root, main root and skin) of Panax quinquefolius L. root (American ginseng) is limited by the lack of efficient differentiation methods. In this study, proteomic technologies were used to establish an advanced protocol suitable for the identification of different parts of P. quinquefolius L. root. Proteins were extracted from four different parts of a 4-year old quinquefolius L. root using optimized sonication and trichloroacetic acid [TCA]/acetone precipitation methods and separated by two-dimensional gel electrophoresis (2-DE). Then 2-DE patterns were matched and analyzed with Image Master 2D Platinum Version 6.0 software. Eight groups of different abundant proteins and 6 specific proteins were inspected (totaling 38 protein spots in all). Further, these proteins were extensively identified by MALDI-TOF-TOF analysis. According to the biological functions, a total of 24 successfully identified proteins could be divided into 5 groups, which were stress response related proteins, energy metabolism related proteins, storage related proteins, hypothesis proteins and unknown proteins. From these results it was concluded that proteomic analysis method was an effective way to identify the different parts of quinquefolius L. root. These findings may contribute to further understanding of the physiological mechanisms of quinquefolius L. © 2011 Academic Journals. Source


Wang Y.,Jilin Agricultural University | Wang C.,Institute of Special Wild Economic Animal and Plant Science | Gao J.,Jilin Agricultural University | Liu C.,Jilin Agricultural University | And 2 more authors.
Environmental Monitoring and Assessment | Year: 2015

Supervised field trials at two locations in 2012 and 2013 were conducted to evaluate the dissipation, terminal residues, and safety evaluation of Nativo 75 water dispersible granule (WG) (25 % trifloxystrobin + 50 % tebuconazole) on ginseng and soil following foliar application at a recommended dose 150 (50 + 100) and 1.5 times of the recommended dosage 225 (75 + 150) g a.i. ha−1. The average recoveries of trifloxystrobin and tebuconazole at three spiking levels in ginseng root, stem, and leaf and in soil were in the ranges of 81.0–96.8 % and 80.2–97.5 % with relative standard deviations (RSDs) of 4.92–13.13 % and 4.67–8.35 %, respectively. The half-lives of trifloxystrobin and tebuconazole were 5.92–9.76 days and 4.59–7.53 days, respectively. The terminal residues were all below the maximum residue limits (MRLs) of EU, USA, Canada, Japan, and South Korea. The food safety was evaluated by comparing the estimated daily intake (IEDI) with its acceptable daily intake (ADI). IEDI values calculated from residue data were found to be far less than the ADI on ginseng. Therefore, it would be unlikely to cause health problems induced by Nativo 75 WG use on ginseng at a dosage of 150–225 g a.i. ha−1. © 2015, Springer International Publishing Switzerland. Source


Shao X.Q.,Institute of Special Wild Economic Animal and Plant Science | Yan X.J.,Institute of Special Wild Economic Animal and Plant Science | Luo G.L.,Institute of Special Wild Economic Animal and Plant Science | Zhang H.L.,Institute of Special Wild Economic Animal and Plant Science | And 9 more authors.
Epidemiology and Infection | Year: 2011

An outbreak of rabies affected domestic raccoon dogs on an animal farm in Inner Mongolia, China in 2007. A study was conducted to characterize the aetiological agent and clarify the origin of the rabies virus. Brain tissues were obtained from five rabid raccoon dogs. Viral nucleoprotein antigen was detected in the brain tissues and five rabies viruses were isolated from these rabid animals. Phylogenetic analysis of the N and G gene sequences showed that these isolates were closely related to Arctic-like rabies viruses isolated from the far-eastern region of Russia and South Korea, but distinct from the rabies viruses that are widely distributed in endemic areas in China. Epidemiological data suggested that the likely source of infection was from one wild raccoon dog that was captured and placed in the same type of pen used for domestic raccoon dogs. © Copyright Cambridge University Press 2010. Source

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