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Al Jīzah, Egypt

Bazaid S.A.,Taif University | Mohamed Muneera A.S.,Taif University | Hussein Sonya H.M.,Taif University | Hussein Sonya H.M.,Institute of Soil Water | And 2 more authors.
Pakistan Journal of Biotechnology | Year: 2011

We are herein focusing on molecular analysis of Argemone plants collected from open area (Al-Shafa, Jabajeb and Al-Arafah), dams (Gadeer Albanat and Ekrima) and valleys (Thumalah, Wadi-Sa'b, Saysid and Wadi-Jaleel) at Taif Governorate in Kingdom of Saudi Arabia. At the level of molecular analyses, DNA fingerprinting of nine Argemone plant samples (1-9) via RAPD-PCR using 10 random primers was determined. Data showed that total fragments of 108 (28 polymorphic fragments+80 monomorphic fragments) were amplified. Out of the 108 fragments, PS21 samples showed 84, 82, 84, 84, 83, 87, 85, 85 and 83, respectively. A number of 14 unique fragments out of the 108 were obtained using the ten RAPD-PCR primers, and could be used as DNA markers. The dendrogram based on RAPD-PCR analysis of the nine PS21 samples showed the presence of four clusters (A, B, C and D) with highest and lowest similarities of 98 and 89%, respectively. Results of SDS-PAGE analysis of the applied PS21 samples was in harmony with that of RAPD-PCR analysis. Results paid an attention to the availability of RAPD-PCR technique as one of the molecular tool for determining the DNA fingerprinting of such important plants. Source

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