Zhou Z.-Q.,Chinese PLA General Hospital |
Zhou Z.-Q.,Institute of Skin Damage and Repair |
Sui Z.-F.,Institute of Skin Damage and Repair |
Tian Y.-L.,Institute of Skin Damage and Repair |
And 4 more authors.
International Journal of Clinical and Experimental Medicine | Year: 2016
Background: To investigate effects of leptin on ultraviolet-induced skin photoaging in human skin fibroblasts (HSFs) and mice and the underlying mechanism. Methods: HSFs in 3-5 subcultures and 50 CD-1 (ICR) mice were randomly divided into blank group, model group and leptin-treated groups. Changes of HSFs and HE stained mouse skin tissue structure exposed to UVA/B irradiation were observed. Senescence-associated β-galactosidase staining of HSFs was determined. Activity of CAT, SOD and GSH-Px and concentrations of MDA, Hyp and LDH were determined by biochemical analysis. Expressions of p67phox, PKCε and p66Shc were detected by Western blotting. Results: UV-induced photoaging models were successfully established. In HSFs, compared with blank group, significantly decreased SA β-gal staining rate and concentrations of CAT, SOD, GSH-Px and Hyp but significantly increased concentrations of MDA, LDH and ROS were found in model group; compared with model group, significantly increased concentrations of CAT, SOD, GSH-Px and Hyp and significantly decreased MDA, LDH and ROS were found in leptin-treated groups. Similar trends were observed regarding the concentrations of CAT, SOD, GSH-Px, Hyp, MDA, LDH and ROS in mouse skin tissues. In both HSFs and mouse skin tissues, the expressions of NADPH, p67phox, PKCε and p66Shc were significantly up-regulated in model group compared with blank group; while the expressions of p67phox, PKCε and p66Shc were significantly down-regulated in leptin-treated groups in comparison with model group. Conclusion: Leptin may prevent skin photoaging by scavenging free radicals, improving antioxidant capacity and enzyme activity, alleviating oxidative damage and promoting collagen synthesis in HSFs and mouse skin tissues. © 2016, E-Century Publishing Corporation. All rights reserved.