Institute of Shandong River Wetlands

Laiwu, China

Institute of Shandong River Wetlands

Laiwu, China
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Zhang J.,Nanjing Southeast University | Zhang J.,Shandong Academy of Agricultural Sciences | Liu C.,Shandong Academy of Agricultural Sciences | Huang R.-Z.,Nanjing Southeast University | And 5 more authors.
PLoS ONE | Year: 2017

Three new lupane-triterpenoids (1-3) along with six known compounds (4-9) were isolated from the ethanolic extract of whole plant of Potentilla discolorBunge. The structures of Compounds 1-3 were established by extensive 1D and 2D NMR together with other spectrum analysis, indicating that their C-27 positions were highly oxygenated, which were rarely found in nature. Their in vitro antiproliferative activities against HepG-2, MCF-7 and T-84 cell lines were evaluated by Cell Counting Kit-8 (CCK-8) assay, and the results showed different activities for three cell lines with IC50 values ranging from 17.84 to 40.64 μM. In addition, the results from Hoechst 33258 and AO/EB staining as well as annexinV-FITC assays exhibited Compound 1 caused a markedly increased HepG-2 cellular apoptosis in a dose-dependent manner. The further mechanisms of Compound 1-induced cellular apoptosis were confirmed that 1 induced the production of ROS and the alteration of pro- and anti-apoptotic proteins, which led to the dysfunction of mitochondria and activation of caspase-9 and caspase-3 and finally caused cellular apoptosis. These results would be useful in search for new potential antitumor agents and for developing semisynthetic lupane-triterpenoid derivatives with high antitumor activity. © 2017 Zhang et al.This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Wang J.L.,CAS Institute of Genetics and Developmental Biology | Wang J.L.,Institute of Shandong River Wetlands | Wang Y.N.,CAS Institute of Genetics and Developmental Biology | Liu D.C.,CAS Institute of Genetics and Developmental Biology | And 6 more authors.
Cereal Research Communications | Year: 2015

Aegiolops kotschyi cytoplasmic male sterile system often results in part of haploid plants in wheat (Triticum aestivum L.). To elucidate the origin of haploid, 235 wheat microsatellite (SSR) primers were randomly selected and screened for polymorphism between haploid (2n = 3x = 21 ABD) and its parents, male-sterile line YM21 (2n = 6x = 42 AABBDD) and male fertile restorer YM2 (2n = 6x = 42 AABBDD). About 200 SSR markers yielded clear bands from denatured PAGE, of which 180 markers have identifiable amplification patterns, and 20 markers (around 8%) resulted in different amplification products between the haploid and the restorer, YM2. There were no SSR markers that were found to be distinguishable between the haploid and the male sterile line YM21. In addition, different distribution of HMW-GS between endosperm and seedlings from the same seeds further confirmed that the haploid genomes were inherited from the maternal parent. After haploidization, 1.7% and 0.91% of total sites were up- and down-regulated exceeding twofold in the shoot and the root of haploid, respectively, and most of the differentially expressed loci were up/down-regulated about twofold. Out of the sensitive loci in haploid, 94 loci in the shoot, 72 loci in the root can be classified into three functional subdivisions: biological process, cellular component and molecular function, respectively.

Wang J.-L.,CAS Institute of Genetics and Developmental Biology | Wang J.-L.,Institute of Shandong River Wetlands | Liu D.-C.,CAS Institute of Genetics and Developmental Biology | Guo X.-L.,China Agricultural University | And 3 more authors.
Journal of Integrative Agriculture | Year: 2014

The introduction of reduced height (Rht) genes Rht-B1b and Rht-D1b led to impressive increases in wheat (Triticum aestivum L.) yields during the Green Revolution. In the present study, the dynamic elongation of peduncle in a set of near-isogenic lines (NILs) carrying different Rht alleles (Rht-B1b, Rht-D1b, Rht-B1c, Rht-D1b+Rht-B1b, and Rht-D1b+Rht-B1c) were investigated. The reduction of the final length of peduncle in NILs was dependent mainly on the elongation rate, which was reduced by Rht genes, during rapid elongation phase. Resin sections showed that Rht genes strongly reduced the cell extension in peduncle. The expression of expansin genes, which mediate cell wall loosening and leading to cell expansion, were analysed by using real-time quantitative PCR (qPCR). Among the 23 possible wheat expansin genes, 17 were expressed in the peduncle. The spatial distribution of expression was further analysed for five expansins that showed high expression levels in the peduncles of Rht lines. Compared to wild type plants, the incorporation of Rht-D1b allele decreased about 37 and 80% of the expression levels of ExpA7 and ExpA3 in elongation zone, respectively. The presence Rht-B1c dwarfing genes, however, produced 53% reduction in the expression level of ExpA7, and seriously decreased about 70% of ExpB9 expression. Although the expression levels of five genes exhibited variability among the lines, an expansin gene, ExpB2, showed its expression level highly associated with the cell elongation rate in peduncle of different Rht lines. © 2014 Chinese Academy of Agricultural Sciences.

Wang J.,China Institute of Water Resources and Hydropower Research | Wang J.,Institute of Shandong River Wetlands | Wang F.,China Institute of Water Resources and Hydropower Research | Chu L.,China Institute of Water Resources and Hydropower Research | And 6 more authors.
PLoS ONE | Year: 2014

Saline lakes are intriguing ecosystems harboring extremely productive microbial communities in spite of their extreme environmental conditions. We performed a comprehensive analysis of the genetic diversity (18S rRNA gene) of the planktonic microbial eukaryotes (nano-and picoeukaryotes) in six different inland saline lakes located in the Qaidam Basin. The novelty level are high, with about 11.23% of the whole dataset showing ,90% identity to any previously reported sequence in GenBank. At least 4 operational taxonomic units (OTUs) in mesosaline lakes, while up to eighteen OTUs in hypersaline lakes show very low CCM and CEM scores, indicating that these sequences are highly distantly related to any existing sequence. Most of the 18S rRNA gene sequence reads obtained in investigated mesosaline lakes is closely related to Holozoa group (48.13%), whereas Stramenopiles (26.65%) and Alveolates (10.84%) are the next most common groups. Hypersaline lakes in the Qaidam Basin are also dominated by Holozoa group, accounting for 26.65% of the total number of sequence reads. Notably, Chlorophyta group are only found in high abundance in Lake Gasikule (28.00%), whereas less represented in other hypersaline lakes such as Gahai (0.50%) and Xiaochaidan (1.15%). Further analysis show that the compositions of planktonic eukaryotic assemblages are also most variable between different sampling sites in the same lake. Out of the parameters, four show significant correlation to this CCA: altitude, calcium, sodium and potassium concentrations. Overall, this study shows important gaps in the current knowledge about planktonic microbial eukaryotes inhabiting Qaidam Basin (hyper) saline water bodies. The identified diversity and novelty patterns among eukaryotic plankton assemblages in saline lake are of great importance for understanding and interpreting their ecology and evolution. Copyright: © 2014 Wang et al.

Zhong Z.-P.,CAS Institute of Microbiology | Zhong Z.-P.,University of Chinese Academy of Sciences | Liu Y.,CAS Institute of Microbiology | Miao L.-L.,CAS Institute of Microbiology | And 5 more authors.
Applied and Environmental Microbiology | Year: 2016

The prokaryotic community composition and diversity and the distribution patterns at various taxonomic levels across gradients of salinity and physiochemical properties in the surface waters of seven plateau lakes in the Qaidam Basin, Tibetan Plateau, were evaluated using Illumina MiSeq sequencing. These lakes included Lakes Keluke (salinity, < 1 g/liter), Qing (salinity, 5.5 to 6.6 g/liter), Tuosu (salinity, 24 to 35 g/liter), Dasugan (salinity, 30 to 33 g/liter), Gahai (salinity, 92 to 96 g/liter), Xiaochaidan (salinity, 94 to 99 g/liter), and Gasikule (salinity, 317 to 344 g/liter). The communities were dominated by Bacteria in lakes with salinities of < 100 g/liter and by Archaea in Lake Gasikule. The clades At12OctB3 and Salinibacter, previously reported only in hypersaline environments, were found in a hyposaline lake (salinity, 5.5 to 6.6 g/liter) at an abundance of ~1.0%, indicating their ecological plasticity. Salinity and the concentrations of the chemical ions whose concentrations covary with salinity (Mg2+, K+, Cl-, Na+, SO4 2-, and Ca2+) were found to be the primary environmental factors that directly or indirectly determined the composition and diversity at the level of individual clades as well as entire prokaryotic communities. The distribution patterns of two phyla, five classes, five orders, five families, and three genera were well predicted by salinity. The variation of the prokaryotic community structure also significantly correlated with the dissolved oxygen concentration, pH, the total nitrogen concentration, and the PO4 3- concentration. Such correlations varied depending on the taxonomic level, demonstrating the importance of comprehensive correlation analyses at various taxonomic levels in evaluating the effects of environmental variable factors on prokaryotic community structures. Our findings clarify the distribution patterns of the prokaryotic community composition in plateau lakes at the levels of individual clades as well as whole communities along gradients of salinity and ionic concentrations. © 2016, American Society for Microbiology. All Rights Reserved.

Tang X.-T.,Yangzhou University | Zheng F.-S.,Institute of Shandong River Wetlands | Qin J.,Yangzhou University | Lu M.-X.,Yangzhou University | Du Y.-Z.,Yangzhou University
PLoS ONE | Year: 2016

Water chestnut beetle (Galerucella birmanica Jacoby) is a pest of the water chestnut (Trapa natans L.). To analyze the phylogeny and biogeography of the beetle and provide evidence for the origin of T. natans in China, we conducted this by using three mitochondrial genes (COI, COII and Cytb) and nuclear ITS2 ribosomal DNA of G. birmanica. As for mtDNA genes, the beetle could be subdivided into three groups: northeastern China (NEC), central-northern-southern China (CC-NC-SC) and southwestern China (SWC) based on SAMOVA, phylogenetic analyses and haplotype networks. But for ITS2, no obvious lineages were obtained but individuals which were from NEC region clustered into one clade, which might be due to sequence conservation of ITS2. Significant genetic variation was observed among the three groups with infrequent gene flow between groups, which may have been restricted due to natural barriers and events in the Late Pleistocene. Based on our analyses of genetic variation in the CC-NC-SC geographical region, the star-like haplotype networks, approximate Bayesian computation, niche modelling and phylogeographic variation of the beetle, we concluded that the beetle population has been lasting in the lower, central reaches of the Yangtze River Basin with its host plant, water chestnut, which is consistent with archaeological records. Moreover, we speculate that the CC-NC-SC population of G. birmanica may have undergone a period of expansion coincident with domestication of the water chestnut approximately 113,900-126,500 years ago. © 2016 Tang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Wang J.,Basin Water | Wang J.,China Institute of Water Resources and Hydropower Research | Wang J.,Institute of Shandong River Wetlands | Wang F.,Basin Water | Wang F.,China Institute of Water Resources and Hydropower Research
Shengtai Xuebao/ Acta Ecologica Sinica | Year: 2014

Saline lakes are widely distributed on the surface of the earth, with a relatively high density of salt lakes recorded in China. Saline lakes are often productive and comprise an important part of the highland ecosystem, since they harbor many novel prokaryotic microorganisms under harsh environmental conditions. However, little research has been conducted in these systems, and understanding about the community diversity and dynamics of picophytoplankton assemblages in such environments is limited. Picophytoplankton, defined as phytoplankton with a diameter less than 0.2-3 μm and composed of both prokaryotes and eukaryotes, not only play a crucial role for primary productivity in marine ecosystems, but also in saline lake ecosystems. Recent survey data suggest that picophytoplankton contribute from 53%-68% of the productivity in the total phytoplankton community in Lake Bogoriau and Lake Nakuru in East Africa. Biodiversity research of picophytoplankton has mainly focused on hypersaline lakes, such as solar salterns, saline ponds, and soda lakes. The most dominant taxa included Arthrospira, Phormidium, and Aphanothece, known as cyanobacteria in hypersaline lakes. Molecular analyses revealed significant genetic diversity in hyposaline soda lakes. After cloning and sequencing, the results found that the majority of the retrieved sequences belonged to prokaryotic picophytoplankton, while the others showed high similarity with eukaryotic algal plastids. Research on the picophytoplankton community patterns and seasonal succession in saline lakes has been attracting the attention of more and more scientists in recent years. Total dissolved solids were identified as the predominant factor structuring the spatial distribution of picophytoplankton. Light intensity, nutrient composition, and temperature also affected the spatial distribution and community composition of picophytoplankton. In this paper, the biodiversity patterns, spatial distributions, and community succession of picophytoplankton within different geographical regions were compared and summarized through literature reviews. Our results highlight the importance of more comprehensive investigation about the diversity, distribution, and ecological role of picophytoplankton in saline lakes at the national level in China.

Zheng F.,Institute of Shandong River Wetlands | Dong H.,Institute of Shandong River Wetlands | Lv Y.,Institute of Shandong River Wetlands | Bi M.,Institute of Shandong River Wetlands | Chen T.,Institute of Shandong River Wetlands
Journal of Food, Agriculture and Environment | Year: 2013

Cold hardiness of water chestnut beetle (Galerucella birmanica Jacoby) in different overwintering periods in Yangzhou area and that in the same overwintering period in different areas were compared by measuring their super-cooling point (SCP). The results showed that the over-wintering adults had different cold-hardiness at different time. The super-cooling point reached the lowest in middle winter. The over-wintering adults collected from different latitudes also showed some differences in cold-hardiness. The SCP was dropping with the latitudes increasing.

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