Institute of Rural Medicine

Lublin, Poland

Institute of Rural Medicine

Lublin, Poland

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Montelius C.,Lund University | Szwiec K.,Lund University | Kardas M.,Lund University | Kardas M.,University of Silesia | And 6 more authors.
Clinical Nutrition | Year: 2014

Background & aims: Dietary chloroplast thylakoids have previously been found to reduce food intake and body weight in animal models, and to change metabolic profiles in humans in mixed-food meal studies. The aim of this study was to investigate the modulatory effects of thylakoids on glucose metabolism and appetite-regulating hormones during an oral glucose tolerance test in pigs fed a high fat diet. Methods: Six pigs were fed a high fat diet (36 energy% fat) for one month before oral glucose tolerance test (1g/kg d-glucose) was performed. The experiment was designed as a cross-over study, either with or without addition of 0.5g/kg body weight of thylakoid powder. Results: The supplementation of thylakoids to the oral glucose tolerance test resulted in decreased blood glucose concentrations during the first hour, increased plasma cholecystokinin concentrations during the first two hours, and decreased late postprandial secretion of ghrelin. Conclusion: Dietary thylakoids may be a novel agent in reducing the glycaemic responses to high carbohydrate and high glycaemic index foods. Thylakoids may in the future be promising for treatment and prevention of diabetes, overweight and obesity. © 2013 The Authors.


Bayliak M.M.,Precarpathian University | Lylyk M.P.,Precarpathian University | Shmihel H.V.,Precarpathian University | Sorochynska O.M.,Precarpathian University | And 4 more authors.
Journal of Thermal Biology | Year: 2016

Alpha-ketoglutarate (AKG) is an important intermediate in Krebs cycle which bridges the metabolism of amino acids and carbohydrates. Its effects as a dietary supplement on cold tolerance were studied in Drosophila melanogaster Canton S. Two-day-old adult flies fed at larval and adult stages with AKG at moderate concentrations (5-10 mM) recovered faster from chill coma (0 °C for 15 min or 3 h) than control ones. The beneficial effect of AKG on chill coma recovery was not found at its higher concentrations, which suggests hormetic like action of this keto acid. Time of 50% observed mortality after 2 h recovery from continuous cold exposure (-1 °C for 3-31 h) (LTi50) was higher for flies reared on 10 mM AKG compared with control ones, showing that the diet with AKG enhanced insect cold tolerance. In parallel with enhancement of cold tolerance, dietary AKG improved fly locomotor activity. Metabolic effects of AKG differed partly in males and females. In males fed on AKG, there were no differences in total protein and free amino acid levels, but the total antioxidant capacity, catalase activity and low molecular mass thiol content were higher than in control animals. In females, dietary AKG promoted higher total antioxidant capacity and higher levels of proteins, total amino acids, proline and low molecular mass thiols. The levels of lipid peroxides were lower in both fly sexes reared on AKG as compared with control ones. We conclude that both enhancement of antioxidant system capacity and synthesis of amino acids can be important for AKG-promoted cold tolerance in D. melanogaster. The involvement of AKG in metabolic pathways of Drosophila males and females is discussed. © 2016 .


Goncharova K.,Lund University | Goncharova K.,Bogomoletz Institute of Physiology | Skibo G.,Bogomoletz Institute of Physiology | Kovalenko T.,Bogomoletz Institute of Physiology | And 5 more authors.
Nutrition and Diabetes | Year: 2015

Background/Objectives: Aging is associated with many physiological alterations such as changes in metabolism, food intake and brain dysfunction. Possible ways to correct age-related brain dysfunction using dietary treatments still remains undeveloped. The aim of our research was to investigate whether long-term dietary treatment with 2-oxoglutarate (2-OX), which is involved in many regulatory pathways, together with pancreatic-like enzymes of microbial origin (PLEM), which ensure appropriate digestion and absorption of nutrients, affects age-related changes in the brain morphology and cognitive function in old Mongolian gerbils. Materials/methods: Experiment was comprised of two separate studies. Samples of the hippocampus were obtained from male Mongolian gerbils of different ages (n=63 in the first study, n=74 in the second study). Immunohistochemistry was used for visualization of the nestin/NeuN-positive neuronal progenitors. Changes in amount of neural cell adhesion molecules (NCAMs) were estimated using enzyme-linked immunosorbent assay. For assessment of cognitive and sensorimotor functions, the T-maze spontaneous alternation test and the adhesive removal test (ART) were used. The ultrastructure of the CA1 hippocampal area was visualized using transmission electron microscopy. Results: Long-term treatment with 2-OX+PLEM led to a significantly increased amount of nestin/NeuN-positive cells in the CA1 hippocampal area and positive changes in learning and sensorimotor functions. As for synaptic transmission, changes in the spatial distribution of synaptic vesicles, as well as the redistribution of NCAM forms, were observed in the hippocampal synapses of the old gerbils. Conclusions: Taken together, our data show that dietary supplementation with 2-OX+PLEM not only enhances the proliferation and differentiation of neuronal progenitors, but also improves age-related deficits in the morphological and functional state of the brain of old gerbils. Thus, suggesting that a 2-OX+PLEM-enriched diet could also improve brain functions that have deteriorated with age. © 2015 Macmillan Publishers Limited.


Krawczyk P.,Medical University of Lublin | Kucharczyk T.,Medical University of Lublin | Kucharczyk T.,Medical University of Warsaw | Chorostowska-Wynimko J.,Institute of Tuberculosis and Lung Diseases | And 6 more authors.
Onkologia Polska | Year: 2011

Erlotinib and gefitinib are tyrosine kinase inhibitors of EGFR (TKI EGFR), which are widely used in second-line treatment of advances non-small cell lung cancer (NSCLC) as well as in first-line therapy instead of standard chemotherapy in genetically predisposed patients (EGFR gene activated mutation in tumour cells). The analysis of EGFR gene mutation encounter many difficulties, e.g. inaccessibility of histopathological or cytological sample and DNA fragmentation in paraffine-fixed tissue. Estimation of EGFR gene mutation in free plasma DNA and in circulating tumour cells in peripheral blood could be very helpful to solve these problems. It was previously shown that the outcome of TKI EGFR therapy is positively correlated with EGFR gene mutation status estimated in peripheral blood samples. In the case of the heterogenicity of solid tumour, the risk of EGFR mutation detection in unique clone of tumour cells is low in peripheral blood. The new approach into molecular diagnosis of NSCLC is to estimate sensitive methods of EGFR gene status examination as denaturing high-performance liquid chromatography (DHPLC), amplification refractory mutation system PCR (ARMS-PCR) and clamp PCR technique. Copyright © 2011 Cornetis.


Dziechciaz M.,Medical Care Center | Dziechciaz M.,Heath Care Institute | Guty E.,Heath Care Institute | Wojtowicz A.,Heath Care Institute | Filip R.,Institute of Rural Medicine
Annals of Agricultural and Environmental Medicine | Year: 2012

Introduction. The needs of elderly people living in the countryside constitute serious health, social, financial and organizational problems. Aim of the study. To define the needs of elderly people living in the countryside regarding complex living actions. Data collected and methodology. The study was carried out among 89 village citizens from the Podkarpackie Voivodeship (N=55; 61.8% women; N=34; 38.2% men) aged 61-2. Average age in the group was 76.3 (+/ -7.9 years). Research methods were 3 different questionnaires, applied to evaluate: socio-demographic data, occurrence of diseases and rehabilitation usage, mental and intellectual status, as well as the Lawton scale (IADL) assessing complex life activities. Results. 18 subjects (20.2%) were fully functional in the scope of complex everyday activities. The highest number were independent in their financial affairs (N=52; 58.4%), preparation and taking of medicine (N=45; 50.6%), and using the telephone (N=39; 43.8%). Lack of self-reliance was most commonly observed with difficult housework (N=62; 69.7%), shopping (N=55; 61.8%), and walking distances exceeding regular walks (N=46; 51.7%). No relation was observed between gender, usage of social welfare, and self-reliance in complex everyday activities. Deterioration in efficiency in the scope of complex everyday activities was observed which progressed with age, and was worse among the unmarried subjects. A relation between material situation and independence, based on the IADL scale, was confirmed, with the exception of using the telephone. Conclusions. 1). People of old age living in the countryside most often need help with complex everyday housework, shopping, and walking distances exceeding regular walks. 2). With the advancement of age, the subjects need help with all IADL activities increased.


Goncharova K.,Lund University | Goncharova K.,Bogomoletz Institute of Physiology | Ushakova G.,Oles Honchar Dnepropetrovsk National University | Kovalenko T.,Bogomoletz Institute of Physiology | And 4 more authors.
Journal of Functional Foods | Year: 2015

It is postulated that exocrine pancreatic insufficiency (EPI) can evoke neurological disorders. In the present study pancreatic-like enzymes of microbial origin (PLEM) were examined as a functional food component, with the goal of improving cognitive function and brain structure in a pig model of EPI. EPI in the present study induced alterations in the behaviour of the pigs as well as degenerative changes within the morphological structure of the hippocampus. EPI leads to a reduced number of pyramidal neurons and decreased levels of neural cell adhesion molecules (NCAM) in the CA1 area of the hippocampus. Here, we provide evidence that the use of PLEM as a functional food, in the form of dietary supplementation with PLEM for 10 days, restored pig behaviour and the histo-morphology of the hippocampus in EPI pigs. Thus, we suggest that the use of PLEM as a functional food ingredient should be considered in the prevention and/or postponement of the development of EPI-related encephalopathy. © 2015 Elsevier Ltd.


Pierzynowski S.,Lund University | Pierzynowski S.,Institute of Rural Medicine | Ushakova G.,Oles Honchar Dnepropetrovsk National University | Kovalenko T.,Bogomoletz Institute of Physiology | And 9 more authors.
International Journal of Developmental Neuroscience | Year: 2014

The first milk, colostrum, is an important source of nutrients and an exclusive source of immunoglobulins (Ig), essential for the growth and protection from infection of newborn pigs. Colostrum intake has also been shown to affect the vitality and behaviour of neonatal pigs. The objective of this study was to evaluate the effects of feeding colostrum and plasma immunoglobulin on brain development in neonatal pigs. Positive correlations were found between growth, levels of total protein and IgG in blood plasma and hippocampus development in sow-reared piglets during the first 3 postnatal days. In piglets fed an elemental diet (ED) for 24. h, a reduced body weight, a lower plasma protein level and a decreased level of astrocyte specific protein in the hippocampus was observed, as compared to those that were sow-reared. The latter was coincident with a reduced microgliogenesis and an essentially diminished number of neurons in the CA1 area of the hippocampus after 72. h. Supplementation of the ED with purified plasma Ig, improved the gliogenesis and supported the trophic and immune status of the hippocampus.The data obtained indicate that the development of the hippocampus structure is improved by colostrum or an Ig-supplemented elemental diet in order to stimulate brain protein synthesis and its development during the early postnatal period. © 2014 ISDN.


Pluta R.,Polish Academy of Sciences | Jablonski M.,Medical University of Lublin | Czuczwar S.J.,Medical University of Lublin | Czuczwar S.J.,Institute of Rural Medicine
Folia Neuropathologica | Year: 2012

The road to clarity for postischemic dementia mechanisms has been one fraught with a wide range of complications and numerous revisions with a lack of a final solution. Importantly, brain ischemia is a leading cause of death and cognitive impairment worldwide. However, the mechanisms of progressive cognitive decline following brain ischemia are not yet certain. Data from animal models and clinical pioneering studies of brain ischemia have demonstrated an increase in expression and processing of amyloid precursor protein to a neurotoxin oligomeric β-amyloid peptide. Functional and memory brain restoration after ischemic brain injury is delayed and incomplete due to a lesion related increase in the amount of the neurotoxin amyloid protein. Moreover, ischemic injury is strongly accelerated by aging, too. In this review, we will present our current thinking about biogenesis of amyloid from the amyloid precursor protein in ischemic brain injury, and how this factor presents etiological, therapeutic and diagnostic targets that are now under consideration. Progressive injury of the ischemic brain parenchyma may be caused not only by degeneration of selectively vulnerable neurons destroyed during ischemia but also by acute and chronic damage of resistant areas of the brain and progressive damage in the blood-brain barrier. We propose that in postischemic dementia an initial ischemic injury precedes the cerebrovascular and brain parenchyma accumulation of Alzheimer disease related neurotoxin β-amyloid peptide, which in turn amplifies the neurovascular dysfunction triggering focal ischemic episodes as a vicious cycle preceding final neurodegenerative pathology. Persistent ischemic blood-brain barrier insufficiency with accumulation of neurotoxin β-amyloid protein in the brain tissue, especially in extracellular perivascular space and blood-brain barrier microvessels, may gradually, over a lifetime, progress to brain atrophy and to full-blown ischemic dementia with Alzheimer phenotype.


Pluta R.,Polish Academy of Sciences | Kocki J.,Medical University of Lublin | Maciejewski R.,Medical University of Lublin | Ulamek-Koziol M.,Polish Academy of Sciences | And 4 more authors.
Folia Neuropathologica | Year: 2012

In this paper we review the hard-earned data, which present ischemic induction of amyloid precursor protein, preseni lins, apolipoproteins and secretases genes, playing key roles in β-amyloid peptide generation. Presented data are strongly supporting a hypothesis that brain ischemia may be involved in the aetiology of sporadic Alzheimer's disease. Potential contribution and impact of ischemically activated genes on the development of sporadic Alzheimer's disease remain to be established at both genetic and functional levels. The identification of the genes involved in sporadic Alzheimer's disease induced by ischemia will enable to further define the events leading to Alzheimer's disease-related abnormalities. Additionally, knowledge gained from the reviewed studies should facilitate the elaboration of effective treatment and/or prevention of sporadic Alzheimer's disease.


Kocki J.,Medical University of Lublin | Ulamek-Koziol M.,Polish Academy of Sciences | Bogucka-Kocka A.,Medical University of Lublin | Januszewski S.,Polish Academy of Sciences | And 9 more authors.
Journal of Alzheimer's Disease | Year: 2015

The interaction between brain ischemia and Alzheimer's disease (AD) has been intensively investigated recently. Nevertheless, we have not yet understood the nature and mechanisms of the ischemic episodes triggering the onset of AD and how they influence its slow progression. The assumed connection between brain ischemia and the accumulation of amyloid-β (Aβ) peptide awaits to be clearly explained. In our research, we employed a rat cardiac arrest model to study the changes in gene expression of amyloid-β protein precursor (AβPP) and its cleaving enzymes, β- and γ-secretases (including presenilins) in hippocampal CA1 sector, following transient 10-min global brain ischemia. The quantitative reverse-transcriptase PCR assay demonstrated that the expression of all above genes that contribute to Aβ peptide generation was dysregulated during 30 days in postischemic hippocampal CA1 area. It suggests that studied Aβ peptide generation-related genes can be involved in AβPP metabolism, following global brain ischemia and will be useful to identify the molecular mechanisms underpinning that cerebral ischemia might be an etiological cause of AD via dysregulation of AβPP and its cleaving enzymes, β- and γ- secretases genes, and subsequently, it may increase Aβ peptide production and promote the gradual and slow development of AD neuropathology. Our data demonstrate that brain ischemia activates delayed neuronal death in hippocampus in an AβPP-dependent manner, thus defining a new and important mode of ischemic cell death. © 2015 - IOS Press and the authors. All rights reserved.

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