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Krawczyk P.,Medical University of Lublin | Kucharczyk T.,Medical University of Lublin | Kucharczyk T.,Medical University of Warsaw | Chorostowska-Wynimko J.,Institute of Tuberculosis and Lung Diseases | And 6 more authors.
Onkologia Polska | Year: 2011

Erlotinib and gefitinib are tyrosine kinase inhibitors of EGFR (TKI EGFR), which are widely used in second-line treatment of advances non-small cell lung cancer (NSCLC) as well as in first-line therapy instead of standard chemotherapy in genetically predisposed patients (EGFR gene activated mutation in tumour cells). The analysis of EGFR gene mutation encounter many difficulties, e.g. inaccessibility of histopathological or cytological sample and DNA fragmentation in paraffine-fixed tissue. Estimation of EGFR gene mutation in free plasma DNA and in circulating tumour cells in peripheral blood could be very helpful to solve these problems. It was previously shown that the outcome of TKI EGFR therapy is positively correlated with EGFR gene mutation status estimated in peripheral blood samples. In the case of the heterogenicity of solid tumour, the risk of EGFR mutation detection in unique clone of tumour cells is low in peripheral blood. The new approach into molecular diagnosis of NSCLC is to estimate sensitive methods of EGFR gene status examination as denaturing high-performance liquid chromatography (DHPLC), amplification refractory mutation system PCR (ARMS-PCR) and clamp PCR technique. Copyright © 2011 Cornetis.

Goncharova K.,Lund University | Goncharova K.,Bogomoletz Institute of Physiology | Ushakova G.,Oles Honchar Dnepropetrovsk National University | Kovalenko T.,Bogomoletz Institute of Physiology | And 4 more authors.
Journal of Functional Foods | Year: 2015

It is postulated that exocrine pancreatic insufficiency (EPI) can evoke neurological disorders. In the present study pancreatic-like enzymes of microbial origin (PLEM) were examined as a functional food component, with the goal of improving cognitive function and brain structure in a pig model of EPI. EPI in the present study induced alterations in the behaviour of the pigs as well as degenerative changes within the morphological structure of the hippocampus. EPI leads to a reduced number of pyramidal neurons and decreased levels of neural cell adhesion molecules (NCAM) in the CA1 area of the hippocampus. Here, we provide evidence that the use of PLEM as a functional food, in the form of dietary supplementation with PLEM for 10 days, restored pig behaviour and the histo-morphology of the hippocampus in EPI pigs. Thus, we suggest that the use of PLEM as a functional food ingredient should be considered in the prevention and/or postponement of the development of EPI-related encephalopathy. © 2015 Elsevier Ltd.

Pluta R.,Polish Academy of Sciences | Jablonski M.,Medical University of Lublin | Czuczwar S.J.,Medical University of Lublin | Czuczwar S.J.,Institute of Rural Medicine
Folia Neuropathologica | Year: 2012

The road to clarity for postischemic dementia mechanisms has been one fraught with a wide range of complications and numerous revisions with a lack of a final solution. Importantly, brain ischemia is a leading cause of death and cognitive impairment worldwide. However, the mechanisms of progressive cognitive decline following brain ischemia are not yet certain. Data from animal models and clinical pioneering studies of brain ischemia have demonstrated an increase in expression and processing of amyloid precursor protein to a neurotoxin oligomeric β-amyloid peptide. Functional and memory brain restoration after ischemic brain injury is delayed and incomplete due to a lesion related increase in the amount of the neurotoxin amyloid protein. Moreover, ischemic injury is strongly accelerated by aging, too. In this review, we will present our current thinking about biogenesis of amyloid from the amyloid precursor protein in ischemic brain injury, and how this factor presents etiological, therapeutic and diagnostic targets that are now under consideration. Progressive injury of the ischemic brain parenchyma may be caused not only by degeneration of selectively vulnerable neurons destroyed during ischemia but also by acute and chronic damage of resistant areas of the brain and progressive damage in the blood-brain barrier. We propose that in postischemic dementia an initial ischemic injury precedes the cerebrovascular and brain parenchyma accumulation of Alzheimer disease related neurotoxin β-amyloid peptide, which in turn amplifies the neurovascular dysfunction triggering focal ischemic episodes as a vicious cycle preceding final neurodegenerative pathology. Persistent ischemic blood-brain barrier insufficiency with accumulation of neurotoxin β-amyloid protein in the brain tissue, especially in extracellular perivascular space and blood-brain barrier microvessels, may gradually, over a lifetime, progress to brain atrophy and to full-blown ischemic dementia with Alzheimer phenotype.

Goncharova K.,Lund University | Goncharova K.,Bogomoletz Institute of Physiology | Skibo G.,Bogomoletz Institute of Physiology | Kovalenko T.,Bogomoletz Institute of Physiology | And 5 more authors.
Nutrition and Diabetes | Year: 2015

Background/Objectives: Aging is associated with many physiological alterations such as changes in metabolism, food intake and brain dysfunction. Possible ways to correct age-related brain dysfunction using dietary treatments still remains undeveloped. The aim of our research was to investigate whether long-term dietary treatment with 2-oxoglutarate (2-OX), which is involved in many regulatory pathways, together with pancreatic-like enzymes of microbial origin (PLEM), which ensure appropriate digestion and absorption of nutrients, affects age-related changes in the brain morphology and cognitive function in old Mongolian gerbils. Materials/methods: Experiment was comprised of two separate studies. Samples of the hippocampus were obtained from male Mongolian gerbils of different ages (n=63 in the first study, n=74 in the second study). Immunohistochemistry was used for visualization of the nestin/NeuN-positive neuronal progenitors. Changes in amount of neural cell adhesion molecules (NCAMs) were estimated using enzyme-linked immunosorbent assay. For assessment of cognitive and sensorimotor functions, the T-maze spontaneous alternation test and the adhesive removal test (ART) were used. The ultrastructure of the CA1 hippocampal area was visualized using transmission electron microscopy. Results: Long-term treatment with 2-OX+PLEM led to a significantly increased amount of nestin/NeuN-positive cells in the CA1 hippocampal area and positive changes in learning and sensorimotor functions. As for synaptic transmission, changes in the spatial distribution of synaptic vesicles, as well as the redistribution of NCAM forms, were observed in the hippocampal synapses of the old gerbils. Conclusions: Taken together, our data show that dietary supplementation with 2-OX+PLEM not only enhances the proliferation and differentiation of neuronal progenitors, but also improves age-related deficits in the morphological and functional state of the brain of old gerbils. Thus, suggesting that a 2-OX+PLEM-enriched diet could also improve brain functions that have deteriorated with age. © 2015 Macmillan Publishers Limited.

Dziechciaz M.,Medical Care Center | Dziechciaz M.,Heath Care Institute | Guty E.,Heath Care Institute | Wojtowicz A.,Heath Care Institute | Filip R.,Institute of Rural Medicine
Annals of Agricultural and Environmental Medicine | Year: 2012

Introduction. The needs of elderly people living in the countryside constitute serious health, social, financial and organizational problems. Aim of the study. To define the needs of elderly people living in the countryside regarding complex living actions. Data collected and methodology. The study was carried out among 89 village citizens from the Podkarpackie Voivodeship (N=55; 61.8% women; N=34; 38.2% men) aged 61-2. Average age in the group was 76.3 (+/ -7.9 years). Research methods were 3 different questionnaires, applied to evaluate: socio-demographic data, occurrence of diseases and rehabilitation usage, mental and intellectual status, as well as the Lawton scale (IADL) assessing complex life activities. Results. 18 subjects (20.2%) were fully functional in the scope of complex everyday activities. The highest number were independent in their financial affairs (N=52; 58.4%), preparation and taking of medicine (N=45; 50.6%), and using the telephone (N=39; 43.8%). Lack of self-reliance was most commonly observed with difficult housework (N=62; 69.7%), shopping (N=55; 61.8%), and walking distances exceeding regular walks (N=46; 51.7%). No relation was observed between gender, usage of social welfare, and self-reliance in complex everyday activities. Deterioration in efficiency in the scope of complex everyday activities was observed which progressed with age, and was worse among the unmarried subjects. A relation between material situation and independence, based on the IADL scale, was confirmed, with the exception of using the telephone. Conclusions. 1). People of old age living in the countryside most often need help with complex everyday housework, shopping, and walking distances exceeding regular walks. 2). With the advancement of age, the subjects need help with all IADL activities increased.

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