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Abramova N.,Institute Microelectronica Of Barcelona Imb Cnm | Bratov A.,Institute Microelectronica Of Barcelona Imb Cnm | Ipatov A.,Institute Microelectronica Of Barcelona Imb Cnm | Levichev S.,Saint Petersburg State University | And 2 more authors.
Talanta | Year: 2013

An analytical system based on ion-selective field effect transistors (ISFETs) with Ca2+ ion selective photocurable membranes is developed to offer a semiautomatic analysis of serum calcium concentration of ruminants. Optimisation of ion-sensitive membrane composition containing different copolymerised plasticizers was performed to minimise the effect of a highly lipophilic samples on sensors characteristics. To improve the system reliability the set of calibration solutions and measurement sequence were also optimised. The system was used to determine total calcium concentration in bovine sera. The precision of the ion determination was higher than reported for double charge ions with a standard deviation of about 3-7%. It is shown that the presence of coagulant in blood serum samples does not affect the determined total calcium concentration. © 2013 Elsevier B.V. Source


Domenech A.,Institute of Research in Agriculture and Technology | Raynes J.G.,London School of Hygiene and Tropical Medicine | Rodriguez E.M.,Institute of Research in Agriculture and Technology | Aris A.,Institute of Research in Agriculture and Technology | And 3 more authors.
Protein and Peptide Letters | Year: 2012

Serum Amyloid A3 (SAA3) protein is a member of a complex group of acute phase and constitutive proteins which have been related to several immune functions. Bovine milk SAA3 (M-SAA3) has been described to have a unique N-terminal TFLK motif responsible for up regulating mucin expression in the intestine lumen and therefore a protective gastrointestinal role. cDNA sequences encoding the protein goat M-SAA3 were successfully cloned from milk, mammary gland tissue and liver, expressed despite observed toxicity and purified as a soluble protein. Sequence analyses of the milk and liver derived forms revealed a non mammary-restricted common N-terminal TFLR motif, unlike that described for bovine M-SAA3. Serum derived forms of SAA have been described to opsonize Gram-negative bacteria facilitating their phagocytosis by circulating macrophages or intestinal epithelial cells. However, no reports about a possible opsonic mechanism of the SAA3 isoforms have been described. Recombinant protein but not peptides encompassing the TFLR region increased blood and milk macrophage interaction and uptake of bacteria reported as number of bacteria per 100 macrophages and percentage of macrophages containing one or more bacteria. gMSAA3-derived peptides did not show any effect on phagocytosis. This would indicate that the TFLK-like region responsible for the up-regulation of mucins in the intestine is not the functional part of g-MSAA3 in promoting macrophage phagocytosis. © 2012 Bentham Science Publishers. Source

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