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São José dos Campos, Brazil

Silva A.,Rajarata University | Pilapitiya S.,Rajarata University | Siribaddana S.,Rajarata University | Siribaddana S.,Institute of Research and Development
BMC Research Notes | Year: 2012

Background: Russells viper (Daboia russelli) bites lead to high morbidity and mortality in South Asia. Although variety of clinical manifestations is reported in viper bite victims, myocardial ischemic events are rare. Case presentation: We report a unique case of inferior wall ST elevation myocardial infarction due to a Russells viper bite over a vein with possible direct intravenous envenoming, in a young male with no past history or family history suggestive of ischemic cardiac disease, from Sri Lanka. In addition, the possible mechanisms of myocardial ischemia in snake bite victims are also briefly discussed. Conclusion: Importance of the awareness of physicians on the rare, yet fatal manifestations of snake envenoming is highlighted. © 2012 Silva et al.; licensee BioMed Central Ltd. Source


Introduction Recombinant activated factor VII (rFVIIa) has been used in the management of traumatic haemorrhage for a decade. Anecdotally, its use is decreasing. The aim of this study was to define the use of rFVIIa in UK deployed military hospitals over the last 5 years. Methods A retrospective database review was performed, using the Joint Theatre Trauma Registry, for the period January 2006 to June 2011. Data collected included use of rFVIIa, injury severity score (ISS), survival and injury pattern. The temporal trend of rFVIIa use, taking into account the number of severely injured patients presenting during each time period, was then analysed. Results During the period January 2006 to June 2011, 156 injured patients received rFVIIa. 146 of these (94%) had an ISS >15; there were 45 fatalities. The median ISS among the group receiving rFVIIa was 30, and 20 patients had an ISS in the range 60-75. There was a significant reduction in the use of rFVIIa in the second half of 2010 and the first half of 2011, compared with the previous 12-month period. Conclusion The use of rFVIIa in UK deployed military hospitals has declined since 2010, which is likely due to a combination of factors, including a change in resuscitation practice in these units, and a change in emphasis of manufacturer's guidance. Source


Pires D.,Institute of Research and Development | Xavier M.,University Camilo Castelo Branco | Araujo T.,University of Sao Paulo | Silva Jr. J.A.,University of Sao Paulo | And 3 more authors.
Lasers in Medical Science | Year: 2011

Low-level laser therapy (LLLT) has been found to produce anti-inflammatory effects in a variety of disorders, Tendinopathies are directly related to unbalance in expression of pro- and anti-inflammatory cytokines which are responsible by degeneration process of tendinocytes, In the current study, we decided to investigate if LLLT could reduce mRNA expression for TNF-α, IL-1β, IL-6, TGF-β cytokines, and COX-2 enzyme, Forty-two male Wistar rats were divided randomly in seven groups, and tendinitis was induced with a collagenase intratendinea injection, The mRNA expression was evaluated by real-time PCR in 7th and 14th days after tendinitis, LLLT irradiation with wavelength of 780 nm required for 75 s with a dose of 7.7 J/cm 2 was administered in distinct moments: 12 h and 7 days post tendinitis, At the 12 h after tendinitis, the animals were irradiated once in intercalate days until the 7th or 14th day in and them the animals were killed, respectively, In other series, 7 days after tendinitis, the animals were irradiated once in intercalated days until the 14th day and then the animals were killed, LLLT in both acute and chronic phases decreased IL-6, COX-2, and TGF-β expression after tendinitis, respectively, when compared to tendinitis groups: IL-6, COX-2, and TGF-β, The LLLT not altered IL-1β expression in any time, but reduced the TNF-α expression; however, only at chronic phase, We conclude that LLLT administered with this protocol reduces one of features of tendinopathies that is mRNA expression for pro-inflammatory mediators. © Springer-Verlag London Ltd 2010. Source


Whitehead S.J.,New Cross Hospital | French J.,Institute of Research and Development | Brookes M.J.,New Cross Hospital | Ford C.,New Cross Hospital | And 2 more authors.
Annals of Clinical Biochemistry | Year: 2013

Background: Faecal calprotectin (f-Cp), a marker of intestinal inflammation, can be used to distinguish between functional and organic bowel disease. F-Cp, following extraction, is commonly quantified using enzyme-linked immunosorbent assays (ELISAs) but there are no data comparing the different f-Cp assays or sample extraction devices. We, therefore, eval-uated and compared the performance of the Immunodiagnostik, Bühlmann and Eurospital f-Cp ELISA assays as well as the Roche, Immunodiagnostik and ScheBo Biotech commercial faecal extraction devices. We also briefly report results from a pilot f-Cp external quality assurance (EQA) scheme. Methods: Imprecision, linearity, recovery, drift and limit of quantitation of the f-Cp assays were evaluated and between-assay variability assessed. The three commercial sample extraction devices were compared with the manual weighing method. Four faecal samples were distributed as part of a pilot EQA scheme to 15 laboratories using quantitative ELISA f-Cp assays. Results: The three f-Cp assays demonstrated adequate intra-/interbatch imprecision, linearity and recovery. The cross-comparison study and EQA data demonstrated that, for the same sample, the Bühlmann assay reports up to 3.8 times higher f-Cp concentrations than the Immunodiagnostik and Eurospital assays. On average, the commercial extraction devices led to a 7.8-28.1% under-recovery of f-Cp in comparison to the manual weighing method. Conclusions: Laboratories should be aware of the lack of the assay standardization, as demonstrated by the between-assay variability. A comparison between f-Cp concentrations reported by these assays and clinical markers of disease severity is required in order to determine their diagnostic accuracy. The EQA scheme represents the first available programme for f-Cp. Source


Meng X.,Ocean University of China | Meng X.,CAS Institute of Botany | Yang L.,Ocean University of China | Kennedy J.F.,Institute of Research and Development | Tian S.,CAS Institute of Botany
Carbohydrate Polymers | Year: 2010

The differences between chitosan (350 kDa) and oligochitosan (6 kDa) in inhibitory effect on phytopathogenic fungi and on decay control were investigated. Both chitosan and oligochitosan strongly inhibited spore germination and mycelial growth of Alternaria kikuchiana Tanaka and Physalospora piricola Nose. Relatively, chitosan and oligochitosan showed more obvious inhibitory effect on mycelial growth than spore germination. Although oligochitosan had better inhibitory effects on fungal pathogenicity in vitro, chitosan was more effective on disease control in pear fruit stored at 25 °C. When treated with oligochitosan, pear fruit increased the activities of chitinase (CHI) and β-1,3-glucanase. Differently, chitosan treatment significantly increased peroxidase (POD) activity of pear fruit. These results suggested that chitosan and oligochitosan triggered different mechanism for pathogenicity inhibition and disease control. © 2010 Elsevier Ltd. All rights reserved. Source

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