Institute of Reproductive Health and Toxicology

Kolkata, India

Institute of Reproductive Health and Toxicology

Kolkata, India

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Mukhopadhyay D.,University of Calcutta | Mukhopadhyay D.,Institute of Reproductive Health and Toxicology | Nandi P.,Institute of Reproductive Health and Toxicology | Nandi P.,University of Calcutta | And 6 more authors.
Fertility and Sterility | Year: 2010

Objective: To evaluate the in vitro effect of benzo[a]pyrene on sperm hyperactivation and acrosome status in normozoospermic semen samples of nonsmokers analyzed by computer-assisted semen analysis (CASA). Design: Experimental in vitro study. Setting: Andrology laboratory. Patient(s): Thirteen proven fertile, normozoospermic, and nonsmoking men. Intervention(s): Spermatozoa were washed free of seminal plasma and were treated with different concentrations of benzo[a]pyrene and compared with controls treated with medium alone. The benzo[a]pyrene concentrations were: 100, 50, 25, and 12.5 μg/mL. Main Outcome Measure(s): Effect of varying concentrations of benzo[a]pyrene on sperm hyperactivation and acrosomal reaction. Result(s): A statistically significant increase in sperm hyperactivation was observed in presence of benzo[a]pyrene at concentrations of ≥50 μg/mL. The result of the acrosome halo test showed that concentrations of benzo[a]pyrene ≥25 μg/mL statistically significantly decreased the percentage of halo formation, indicating an inappropriate (false) acrosome reaction. Conclusion(s): Benzo[a]pyrene statistically significantly affected sperm functional competence as evidenced by increased hyperactivation as well as premature acrosomal reaction. © 2010 American Society for Reproductive Medicine.


Mukhopadhyay D.,University of Calcutta | Mukhopadhyay D.,Institute of Reproductive Health and Toxicology | Varghese A.C.,Institute of Reproductive Health and Toxicology | Varghese A.C.,Advanced Medicare and Research Institute Ltd | And 6 more authors.
Andrologia | Year: 2010

This study was conducted after an initial epidemiological survey of patients in and around Calcutta, India, concerning their lifestyle history, degree of risk exposure and semen analysis based on conventional WHO criteria. It was found that a large group of exposed patients were showing normozoospermic semen parameters in conventional semen analysis. Hence, a selected group of subjects, designated as normozoospermic in routine analysis, but under risk factor exposure, were selected for a repeat computer aided semen analysis (CASA) and were compared with a control group. The parameters considered among CASA results were: curvilinear velocity (VCL), straight-line velocity, average path velocity (VAP), straightness index (STR), lateral head displacement (ALH) and beat cross frequency. The results depict a significant decline in the mean values of VCL (P = 0.029) and STR (P = 0.007) in the tobacco-exposed group when compared with the unexposed group. On the other hand, there was a significant decline in the mean values of VCL (P = 0.014) and ALH (P = 0.040) in the heavy metal-exposed group when compared with the unexposed group. The other parameters did not show significant change in either group. Semen samples that had been designated normozoospermic in conventional analysis were seen to be influenced by risk factors at the level of sperm motion kinetics. © 2010 Blackwell Verlag GmbH.

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