Institute of Public Health Ostrava

Ostrava, Czech Republic

Institute of Public Health Ostrava

Ostrava, Czech Republic
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Agency: European Commission | Branch: FP7 | Program: CP-IP | Phase: NMP.2012.1.3-3 | Award Amount: 49.52M | Year: 2013

The innovative and economic potential of Manufactured Nano Materials (MNMs) is threatened by a limited understanding of the related EHS issues. While toxicity data is continuously becoming available, the relevance to regulators is often unclear or unproven. The shrinking time to market of new MNM drives the need for urgent action by regulators. NANoREG is the first FP7 project to deliver the answers needed by regulators and legislators on EHS by linking them to a scientific evaluation of data and test methods. Based on questions and requirements supplied by regulators and legislators, NANoREG will: (i) provide answers and solutions from existing data, complemented with new knowledge, (ii) Provide a tool box of relevant instruments for risk assessment, characterisation, toxicity testing and exposure measurements of MNMs, (iii) develop, for the long term, new testing strategies adapted to innovation requirements, (iv) Establish a close collaboration among authorities, industry and science leading to efficient and practically applicable risk management approaches for MNMs and products containing MNMs. The interdisciplinary approach involving the three main stakeholders (Regulation, Industry and Science) will significantly contribute to reducing the risks from MNMs in industrial and consumer products. NANoREG starts by analysing existing knowledge (from WPMN-, FP- and other projects). This is combined with a synthesis of the needs of the authorities and new knowledge covering the identified gaps, used to fill the validated NANoREG tool box and data base, conform with ECHAs IUCLID DB structure. To answer regulatory questions and needs NANoREG will set up the liaisons with the regulation and legislation authorities in the NANoREG partner countries, establish and intensify the liaisons with selected industries and new enterprises, and develop liaisons to global standardisation and regulation institutions in countries like USA, Canada, Australia, Japan, and Russia.

Papa A.,Aristotle University of Thessaloniki | Zelena H.,Institute of Public Health Ostrava | Barnetova D.,University of Ostrava | Petrousova L.,University of Ostrava
Clinical Microbiology and Infection | Year: 2010

In the summer of 2008, a 15-year-old boy was hospitalized in a paediatric intensive care unit in the Czech Republic. Laboratory diagnosis of hantavirus infection was established by serological and molecular methods. Sequence and phylogenetic analyses showed that the causative strain was Dobrava/Belgrade virus, which is genetically closer to strains associated with Apodemus flavicollis rodents. © 2010 The Authors. Journal Compilation © 2010 European Society of Clinical Microbiology and Infectious Diseases.

Mills G.A.,University of Portsmouth | Greenwood R.,University of Portsmouth | Vrana B.,Masaryk University | Allan I.J.,Norwegian Institute for Water Research | Ocelka T.,Institute of Public Health Ostrava
Journal of Environmental Monitoring | Year: 2011

Passive sampling devices have been used since the 1970s to measure time-weighted average (TWA) or equilibrium concentrations of pollutants in various environmental matrices (e.g. air, soils and sediments and water). In recent years the popularity of using such samplers has increased and the technology in now well established for the measurement of atmospheric pollutants. This sector has a long experience of using passive samplers in the short- and long-term assessment of air quality in the local environment and on a global scale (e.g. within the United Nations Stockholm Convention on the trans-boundary movement of persistent organic pollutants (POPs) where large networks of samplers on a continental scale have been established). In comparison, the use of passive samplers for monitoring the aquatic environment has been slower to take off. There has, however, been a recent research drive to develop devices for measuring the wide range of pollutants that can be found in environmental waters (e.g. ground, surface, and marine). It is now being recognised that passive samplers can play a valuable role in monitoring water quality within a legislative framework such as the European Union's Water Framework Directive (WFD). The data from these devices can be used alongside the results obtained from conventional spot or bottle sampling to improve risk assessments and to inform decisions on undertaking potentially expensive remedial actions. Such monitoring techniques may have uses within the European Registration, Evaluation, Authorisation & restriction of CHemicals (REACH) Directive and the forthcoming European Marine Strategy Directive. It is expected that the aquatic monitoring sector will follow a transition similar to that which occurred in air monitoring where data obtained from passive samplers can use used within a legal framework. There has also been increased interest in extending the role of passive samplers to both the measurement of equilibrium concentrations and investigating the movement and release of the dissolved fraction of various pollutants in the pore water of sediments and soils. © The Royal Society of Chemistry 2011.

Kurolt I.C.,University of Zagreb | Betica-Radic L.,General Hospital | Dakovic-Rode O.,University of Zagreb | Franco L.,Institute Salud Carlos III | And 3 more authors.
Clinical Microbiology and Infection | Year: 2013

In the summer of 2010, two autochthonous dengue fever cases were detected in Croatia. Here we report the retrospective detection of an additional case of dengue fever, representing the first sustained autochthonous transmission in Europe since 1928. In addition, we present the phylogenetic analyses based on two sequences from the Pelješac peninsula, southern Croatia. The sequences were identified as dengue virus genotype 1 and recovered from two out of the three Pelješac patients in whom infection occurred. © 2012 The Authors Clinical Microbiology and Infection © 2012 European Society of Clinical Microbiology and Infectious Diseases.

Szlinder-Richert J.,Polish National Marine Fisheries Research Institute | Barska I.,Polish National Marine Fisheries Research Institute | Usydus Z.,Polish National Marine Fisheries Research Institute | Grabic R.,Institute of Public Health Ostrava
Chemosphere | Year: 2010

This paper reports concentrations of seven polybrominated diphenyl ether (PBDE) congeners in selected fish species that were collected from the southern Baltic in the 2004-2006 period. Differences in concentrations of PBDEs among the fish species were observed. The mean ∑7PBDE concentrations measured in herring (Clupea harengus), sprat (Sprattus sprattus), and salmon (Salmo salar) samples were 1.2 ng g-1 wet weight, 1.6 ng g-1 wet weight, and 2.5 ng g-1 wet weight, respectively. PBDE-47 was the prevalent congener in all the samples tested. PBDE concentrations in the herring samples tested in the current study were similar to those determined in herring sampled in the northern Baltic, but slightly lower in comparison with concentrations in fish from the Belgian North Sea. PBDE levels occurring in salmon sampled in the southern Baltic were lower than those measured in the northern and northeastern Baltic, but similar to levels determined in salmon from the central part of the Baltic Sea. The risk posed by consuming the fish tested in the current study was evaluated according to the Provisional Tolerable Weekly Intake (PTWI) value for PBDEs recommended by the European Food Safety Authority (EFSA). © 2009 Elsevier Ltd. All rights reserved.

Holesova S.,VSB - Technical University of Ostrava | Samlikova M.,VSB - Technical University of Ostrava | Ritz M.,VSB - Technical University of Ostrava | Pazdziora E.,Institute of Public Health Ostrava
Materials Today: Proceedings | Year: 2015

The set of low density polyethylene/clay nanocomposites with increasing amount of organoclay nanofiller (3, 6 and 10wt %) was prepared by melt compounding procedure without additives. An antimicrobial drug, chlorhexidine diacetate (CA), was loaded into Zn2+ form of clay mineral vermiculite (ZnVer) at very low concentration and then organoclay nanofiller CA/ZnVer was incorporated into polymeric matrix. The structural characteristics of all prepared samples were studied by XRD and FTIR techniques. The nanocomposites were tested for antibacterial activity against Staphylococcus aureus and Escherichia coli. Prepared samples showed a very good antibacterial activity with long lasting effect against S. aureus and slightly worse effect against E. coli. © 2015 Elsevier Ltd.

Holesova S.,VSB - Technical University of Ostrava | Valaskova M.,VSB - Technical University of Ostrava | Plevova E.,Academy of Sciences of the Czech Republic | Pazdziora E.,Institute of Public Health Ostrava | Matejova K.,Institute of Public Health Ostrava
Journal of Colloid and Interface Science | Year: 2010

The novel antibacterial organovermiculites with different mass ratios of chlorhexidine diacetate (CA) were successfully prepared by ion exchange reactions. The resultant organovermiculites were characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR) and thermal properties of prepared organovermiculites were investigated by simultaneous thermogravimetry (TG) and differential thermal analysis (DTA). The antibacterial activity of prepared organovermiculites against Enterococcus faecalis, Escherichia coli and Pseudomonas aeruginosa was evaluated by finding minimum inhibitory concentration (MIC). Antibacterial studies showed that the organovermiculites strongly inhibited the growth of variety of microorganisms. © 2009 Elsevier Inc. All rights reserved.

Grabic R.,Institute of Public Health Ostrava | Grabic R.,Umeå University | Jurcikova J.,Institute of Public Health Ostrava | Tomsejova S.,Institute of Public Health Ostrava | And 4 more authors.
Environmental Toxicology and Chemistry | Year: 2010

Endocrine-disrupting chemicals (EDC) are manmade or natural chemicals that have the ability to interfere with the endocrine system of animals. They have not been monitored systematically in the Czech Republic. The goal of the present study was the characterization of aquatic environmental pollution from the Brno (Czech Republic) city agglomeration focusing on EDC. Passive sampling devices, as well as semipermeable membrane devices (SPMD) and polar organic chemical integrative samplers (POCIS), were used for the pilot assessment of EDC. They were deployed for 21- to 28-d periods at nine locations in the Svratka and Svitava Rivers, Brno, Czech Republic, including at the inlet and outlet of Brno's wastewater treatment plant. The SPMDs were used to monitor nonpolar compounds such as polychlorinated biphenyls (PCBs), polynuclear aromatic hydrocarbons (PAHs), and organochlorinated pesticides (OCP). The POCIS were used to monitor polar compounds such as pesticides and their metabolites, perfluoro-organic compounds (PFOC), and pharmaceuticals. The passive samplers allowed very low detection limits for soluble (bioavailable) fractions of pollutants. The contribution of PAH, PCB, and HCB in sewage water to pollution of the Svratka River was low. The Brno wastewater treatment plant was identified as the main source of pharmaceuticals, triclosan, methyl triclosan, and some polar pesticides. © 2009 SETAC.

Dedkova K.,VSB - Technical University of Ostrava | Matejova K.,Institute of Public Health Ostrava | Lang J.,VSB - Technical University of Ostrava | Peikertova P.,VSB - Technical University of Ostrava | And 4 more authors.
Journal of Photochemistry and Photobiology B: Biology | Year: 2014

The paper addresses laboratory preparation and antibacterial activity testing of kaolinite/nanoTiO2 composite in respect of the daylight irradiation time. Kaolinite/nanoTiO2 composites with 20 and 40 wt% of TiO2 were laboratory prepared, dried at 105 °C and calcined at 600 °C. The calcination caused transformation of kaolinite to metakaolinite and origination of the metakaolinite/nanoTiO2 composite. X-ray powder diffraction, Raman and FTIR spectroscopic methods revealed titanium dioxide only in the form of anatase in all evaluated samples (non-calcined and calcined) and also transformation of kaolinite to metakaolinite after the calcination treatment. Scanning electron microscopy was used as a method for characterization of morphology and elemental composition of the studied samples. A standard microdilution test was used to determine the antibacterial activity using four human pathogenic bacterial strains (Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa). A lamp with a wide spectrum bulb simulating daylight was used for induction of photocatalysis. The antibacterial assays found all the KATI samples to have antibacterial potency with different onset of the activity when calcined samples exhibited antibacterial activity earlier than the non-calcined. Significant difference in antibacterial activity of KATI samples for different bacterial strains was not observed. © 2014 Elsevier B.V. All rights reserved.

Kalicakova Z.,VSB - Technical University of Ostrava | Micka V.,Institute of Public Health Ostrava | Lach K.,Institute of Public Health Ostrava | Danihelka P.,VSB - Technical University of Ostrava
Journal of Physics: Conference Series | Year: 2013

Air pollution harms human health and the environment. Ostrava's agglomeration and its immediate vicinity suffer regular exceeding of air pollution limits due to its geomorphologic location and present heavy industry. Maximum exceedances of air quality standards and especially PM10 which 24 hour limit value is in EU 50μg.m-3, must not be exceeded more than 35 days per year. This limit is being still often exceeded. In the year 2011 such as situation occurred 126 times. It is very important then for identify sources of air pollution to find out maximum information about air borne dust, like size distribution, chemical composition of individual size fractions, morphology of particulate matter together with other parameters like meteorological conditions, year season etc. Our measurement started two years ago. We focus on the critical situation when there are values of PM10 over a long period above the limit. In winter season it is so called inversion. By default, during the campaign it is measured size distribution of air born dust in range 5.6 nm -560nm by FMPS and using the sampler NanoId are collected samples in range 1nm-35μm in 12 size fractions for chemical analysis and morphological observations. This contribution deals with results of size distributions only. © IOP Publishing Ltd 2013.

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