Entity

Time filter

Source Type


Najbauer J.,University of Pecs | Kraljik N.,Institute of Public Health for the Osijek Baranja County | Nemeth P.,University of Pecs
Pathology & Oncology Research | Year: 2014

Malignant gliomas are among the deadliest primary brain tumors. Despite multimodal therapy and advances in chemotherapy, imaging, surgical and radiation techniques, these tumors remain virtually incurable. Glioma stem cells may be responsible for resistance to traditional therapies and tumor recurrence. Therefore, elimination of glioma stem cells may be crucial for achieving therapeutic efficacy. Metformin, a small molecule drug widely used in the therapy of type 2 diabetes, has shown significant anti-tumor effects in patients with breast cancer and prostate cancer. Recent preclinical data suggest that metformin also has therapeutic effects against glioma. Here we review the markers and hallmarks of glioma stem cells, and the molecular mechanisms involved in therapeutic targeting of glioma stem cells by metformin. © 2014 Arányi Lajos Foundation.


Rosso M.,University Hospital Center Osijek | Prgomet D.,University of Zagreb | Marjanovic K.,University Hospital Center Osijek | Puseljic S.,University Hospital Center Osijek | Kraljik N.,Institute of Public Health for the Osijek Baranja County
European Archives of Oto-Rhino-Laryngology | Year: 2015

Total laryngectomy results in a permanent disconnection of the upper and lower airways. Thus, the upper airways are bypassed and can no longer condition, humidify, and filter the inhaled air, leading to damage of the tracheobronchial epithelium. There is little scientific information available about the effects of tracheostoma breathing and the degree of mucosal damage in laryngectomized patients. The aims of this study were to determine the histopathologic findings and investigate the potential impact of using a heat and moisture exchanger (HME) on the tracheal epithelium in long-term tracheostomy patients. Tracheal mucosal biopsies were taken from a total of 70 patients. Specimens were stained with hematoxylin and eosin and examined by a light microscope. Normal pseudostratified ciliated columnar epithelium was found in only 9 (12.9 %) cases; while, 17 (24.3 %) cases had some degree of basal cell hyperplasia. Squamous metaplasia was the most common finding (50 %). Pre-invasive lesions (mild and moderate squamous dysplasia) were found in only one patient who used an HME, and in eight (11.4 %) non-users. Although the HME cannot completely restore the physiological functions of the upper respiratory track, it delivers a better quality of air to the lower airways and has a positive effect on tracheal mucosa. © 2014, Springer-Verlag Berlin Heidelberg.


Miskulin M.,Josip Juraj Strossmayer University of Osijek | Puntaric D.,Josip Juraj Strossmayer University of Osijek | Bozikov J.,University of Zagreb | Miskulin I.,Josip Juraj Strossmayer University of Osijek | Ruzman N.,Institute of Public Health for the Osijek Baranja County
Clinical Laboratory | Year: 2012

Background: The objective of this study is to determine the prevalence of blood donors positive for herpes simplex virus type 2 (HSV-2), to identify the patterns of sexual risk behavior responsible for HSV-2 positivity and to assess the reliability of HSV-2 positivity as a marker of sexual risk behavior in the study population. Methods: This cross-sectional study included 423 blood donors of both sexes from eastern Croatia. Their blood samples were tested by ELISA IgG test kit for HSV-2 IgG and Western blot. Data on sexual risk behavior were collected by use of an anonymous questionnaire. Results: Western blot testing showed HSV-2 IgG antibodies in 14 of 423 (3.3%) donor blood samples. The most common patterns of sexual risk behavior potentially associated with test positivity were irregular condom use during sexual intercourse with new partners (294/423; 69.5%) and ≥5 sexual partners during lifetime (213/423; 50.4%). Conclusions: The population of blood donors from eastern Croatia included subgroups of subjects characterized by sexual risk behavior. Study results pointed to a relationship between various forms of sexual risk behavior and HSV-2 positivity, which could therefore serve as a reliable marker of sexual risk behavior in the study population.


Pastuovic T.,Institute of Public Health for the Osijek Baranja County | Peric M.,Institute of Public Health for the Osijek Baranja County | Bosnjak Z.,Institute of Public Health for the Osijek Baranja County | Ruzman N.,Institute of Public Health for the Osijek Baranja County | And 5 more authors.
Acta medica academica | Year: 2015

OBJECTIVE: The aim of this 5-year study was to determine the frequency and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus (MRSA)-related infections at Osijek Clinical Hospital.MATERIALS AND METHODS: A total of 1987 staphylococci-infected clinical isolates were collected and analysed at the Microbiology Department of the Public Health Institute of Osijek-Baranja County.RESULTS: Between 2008 and 2012, the average rate of MRSA-related infections in staphylococci-infected patients was 27.4%. The proportion of MRSA-related infections on all Staphylococcus aureus (S. aureus) isolates from clinical specimens showed a decreasing trend, from 32.6% in 2008 to 25.5% in 2012. MRSA-related infections were mostly detected in wound swabs (50.6%) and aspirates (28.8%) of patients hospitalized in the surgical (49.8%) and intensive care units (27.9%). MRSA-related infection showed an increase compared to S. aureus-infections in samples of wounds and aspirates in 2011 and 2012 (57.9%/34.9% and 35.2%/16.3%, respectively). The majority of strains of MRSA-related infections were resistant to several antibiotics, including erythromycin and clindamycin, where susceptibility were less than 10%. All MRSA isolates were susceptible to vancomycin, teicoplanin and linezolid. Therefore, antibiotic therapies for MRSA infections include vancomycin, teicoplanin and linezolid, but microbiological diagnostics need to be performed in order to know when the use of glycopeptides and oxazolidinones is indicated.CONCLUSION: Our results suggest that appropriate prevention measures, combined with the more rational use of antibiotics are crucial to reduce the spread of MRSA-related infection in healthcare settings. Further monitoring is necessary of the incidence and antibiotic susceptibility of MRSA-related infections in our community. Copyright © 2015 by Academy of Sciences and Arts of Bosnia and Herzegovina.


Miskulin M.,Josip Juraj Strossmayer University of Osijek | Puntaric D.,Josip Juraj Strossmayer University of Osijek | Miskulin I.,Josip Juraj Strossmayer University of Osijek | Atalic B.,Josip Juraj Strossmayer University of Osijek | Dijanic T.,Institute of Public Health for the Osijek Baranja County
Blood Transfusion | Year: 2011

Background. In this cross-sectional study we assessed the prevalence of the various blood safety sexual risk behaviours among blood donors from East Croatia and the possibilities for improving the present blood donor screening procedure. Materials and methods. The study included 423 blood donors of both sexes who completed a specially designed anonymous questionnaire immediately after their blood donations. The questionnaire contained questions on demographic data, sexual preferences, and possible sexual risk behaviours in the period before the blood donation. Results. The study revealed that the most common forms of sexual risk behaviour among Croatian blood donors had been irregular condom use during sexual contacts with new partners (294/423; 69.5%) and five or more sexual partners during the lifetime (213/423; 50.4%). More male than female subjects had had five or more sexual partners during their lifetime (p<0.0001). Compared to married donors, single subjects more frequently reported sexual contacts without using a condom with their new partners in the period from the last blood donation (p<0.0001) and also in the period of 1 month before current blood donation (p=0.0060). A larger number of younger than older subjects had practiced different types of sexual intercourses during the lifetime (p=0.0002) but more older than younger subjects reported irregular use of condom on sexual contacts with a new partner during their lifetime (p=0.0231). Conclusion. Blood donors who were involved in various forms of sexual risk behaviour represent a serious threat to the health of blood recipients. In this respect a new approach to blood donor screening procedure with additional questions on sexual behaviour in anonymous donor questionnaires would improve the safety of transfusion treatment in East Croatia. © SIMTI Servizi Srl.

Discover hidden collaborations