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Chakraborty M.,University of Calcutta | Bhattacharya S.,University of Calcutta | Bose M.,University of Calcutta | Sasmal S.K.,Institute of Post Graduate Ayurvedic Education and Research | And 2 more authors.
Indian Journal of Experimental Biology | Year: 2017

Aqueous extract of freshwater mussel, Lamellidens marginalis is known to possess potent antioxidant and anti-inflammatory activity. Here, we have made an attempt to purify anti-inflammatory protein from Lamellidens marginalis extract (LME). Aqueous LME was prepared, and total protein was precipitated by 60% ammonium sulfate followed by purification through ion exchange chromatography. Isolated fractions were studied for anti-inflammatory activity in in vitro and in vivo experimental models. Active fractions were characterized by SDS PAGE and HPLC. Protein recovered from ammonium sulfate precipitation showed four distinct peaks in diethyl-aminoethyl cellulose ion exchange chromatography when eluted with stepwise salt gradient. Protein fraction eluted in 0.5 M sodium chloride solution showed maximum specific activity and anti-inflammatory activity in acute model and adjuvant induced chronic inflammation model. This fraction also showed cyclo-oxygenase 2 (COX2) enzyme inhibitory activity in in-vitro system. In SDS-PAGE 0.5 M NaCl fraction showed multiple bands after Coomassie brilliant blue staining and three distinct peaks in HPLC. In this study, we identified an anti-inflammatory protein fraction with high anionic property which could be attributed to inhibition of COX2 enzyme activity. © 2017, National Institute of Science Communication. All rights reserved.


Gupta M.,Institute of Post | Shaw B.,Institute of Post Graduate Ayurvedic Education and Research
Iranian Journal of Pharmaceutical Research | Year: 2011

Asparagus racemosus Willd. has repeatedly been mentioned as a galactogogue in Ayurvedic literature and has been confirmed through animal experiments as well. This randomized double-blind clinical trial evaluates its galactogogue effect in 60 lactating mothers by measurement of changes in their prolactin hormone level during the study. Several secondary parameters namely mothers' weight, babies' weight, subjective satisfaction of mothers and well-being and happiness of babies were studied to corroborate the primary findings. The oral administration of the research drug led to more than three-fold increase in the prolactin hormone level of the subjects in the research group as compared to the control group. The primary findings were corroborated by the secondary outcome measures and were found to be statistically significant (p < 0.05). © 2011 by School of Pharmacy.


Sasmal S.,Institute of Post Graduate Ayurvedic Education and Research | Majumdar S.,Institute of Post Graduate Ayurvedic Education and Research | Gupta M.,Institute of Post Graduate Ayurvedic Education and Research | Mukherjee A.,University of Calcutta
Asian Pacific Journal of Tropical Biomedicine | Year: 2012

Objective: To conduct a systemic evaluation of the medicinal value of seeds which include macroscopic and microscopic characterization, physiochemical evaluation, preliminary phytochemical screening and experimental antipyretic activity. Methods: Saraca asoca seed was studied for pharmacognostical, phytochemical and other recommended methods for standardizations. Also, the acetone extract of the seeds was evaluated for acute toxicity study and antipyretic activity using Brewer's yeast induced pyrexia in Wistar rats at oral doses of 300 mg/kg and 500 mg/kg. Results: After phytochemical screening, the acetone extract showed the presence of saponin, tannins and flavonoids which inhibit pyrexia. The therapeutic efficacy achieved at both the dose levels of the research drug and standard drug aspirin (100 mg/kg) showed significant (. P<0.01) antipyretic activity when compared to the control group. The highly significant antipyretic effect exhibited at the dose of 500 mg/kg was also found to be sustainable in nature. Conclusion: The antipyretic effect of the acetone extract showed significant results in rats at the dose of 500 mg/kg after following the standard pharmacognostical and phytochemical methods. © 2012 Asian Pacific Tropical Biomedical Magazine.


Sarkar P.K.,Jb Roy State Ayurvedic Medical College And Hospital | Chaudhari S.,Jb Roy State Ayurvedic Medical College And Hospital | Chattopadhyay A.,Institute of Post Graduate Ayurvedic Education and Research
Drug Metabolism and Drug Interactions | Year: 2013

Ayurvedic medicines are available in the market as over-the-counter products. Today people use prescription and nonprescription medicines along with Ayurvedic medicines for quick relief from ailments. In the ancient texts of Ayurveda, the concept of interactions with various examples of food interactions and food-drug interactions are mentioned. Recent studies and publications reported drug interactions of Ayurveda medicines and modern drugs. In the present review article, the concept of interactions mentioned in the Ayurvedic texts along with the examples of food interactions, food-drug interactions and the recent research work and publications indicating the interactions of the Ayurvedic drugs and drug interactions of Ayurvedic medicines and modern drugs are compiled. This will help the consumer of the prescription and nonprescription medicines with the Ayurvedic medicines to be cautious about the probable interactions.


Gupta M.,Institute of Post Graduate Ayurvedic Education and Research
International Journal of Food Properties | Year: 2010

Indian spices that provide flavor, color, and aroma to food also possess many therapeutic properties. Ancient Indian texts of Ayurveda, an Indian system of medicine, detailed the medicinal properties of these plants and their therapeutic usage. Recent scientific research has established the presence of many active compounds in these spices that are known to possess specific pharmacological properties. The therapeutic efficacy of these individual spices for specific pharmacological actions has also been established by experimental and clinical studies. The medicinal effects traditionally ascribed to Indian spices are validated by modern pharmacological and experimental techniques, thus providing a scientific rationale to their traditional therapeutic usage. Copyright © 2010 Taylor & Francis Group, LLC.


Gupta M.,Institute of Post Graduate Ayurvedic Education and Research
International Journal of Pharma and Bio Sciences | Year: 2010

Degenerative physiological changes related to old-age are increasing world over. These geriatric diseases affect almost all vital body systems. The rejuvenating and preventive therapy called Rasayana therapy in Ayurvedic system of Indian medicine deals with prevention, amelioration and cure of geriatric ailments by increasing overall body immunity, fighting infections & antigens, and preventing carcinogenic mutations. A specific polyherbal preparation called Triphala, which consists of equal amounts of fruits of three plants namely Terminalia chebula Retz., Terminalia bellirica Roxb. and Emblica officinalis Gaertn. in fine powder form, has been specifically mentioned in traditional Ayurvedic texts for its beneficial effects in geriatric diseases. It contains tannins, phenols and glycosides which are responsible for its strong antioxidant activity apart from its immunomodulatory, anti-inflammatory, analgesic and antimutagenic properties. These attributes make Triphala an effective remedy for geriatric degenerative diseases.


PubMed | Institute of Post Graduate Ayurvedic Education and Research
Type: Journal Article | Journal: International journal of Ayurveda research | Year: 2010

The aqueous extract of Jwarhar mahakashay Ayurvedic preparation (from the roots of Hemidesmus indicus R. Br., Rubia cordifolia L., Cissampelos pareira L.; fruits of Terminalia chebula Retz., Emblica officinalis Gaertn., Terminalia bellirica Roxb., Vitis vinifera L., Grewia asiatica L., Salvadora persica L. and granules of Saccharum officinarum L.) has been used as a traditional antipyretic. Experimental studies confirmed its antipyretic-analgesic effect with very low ulcerogenicity and toxicity. Flavonoids, glycosides and tannins were later found to be present in the extract. Detailed chemical investigations were undertaken after hydrolysis of extract using spectroscopic and chromatography methods to determine its active chemical constituent. UV-Visible spectroscopy showed absorbance maxima at 220 and 276 nm, while fourier transform infra-red investigations indicated an end carboxylic O-H structure at 2940 cm(-1) suggesting the presence of glycoside-linked flavonoids. Thin layer chromatography and high performance liquid chromatography also confirmed the possibility of at least one major and two minor compounds in this abstract. Detailed examination using gas chromatography-mass spectrometry led to the identification of the principal component as 2-(1-oxopropyl)-benzoic acid, which is quite similar to the active compound found in the standard drug Aspirin (2-acetyl-oxybenzoic acid).

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