Institute of Plant Genetic Resources

Sadovo, Bulgaria

Institute of Plant Genetic Resources

Sadovo, Bulgaria
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Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: KBBE-2008-1-2-01 | Award Amount: 3.92M | Year: 2009

Organic and low-input farming systems have been shown to benefit farmland biodiversity although a generic indicator system to assess these benefits at the European level is lacking. The BIOBIO project will therefore pursue the following objectives: 1. Conceptualization of criteria for a scientifically-based selection of biodiversity indicators for organic/low-input farming systems; 2. Assessment and validation of a set of candidate biodiversity indicators in representative case studies across Europe (and in ICPC countries); 3. Preparation of guidelines for the implementation of biodiversity indicators for organic/low-input farming systems for Europe and beyond. Existing indirect farm management indicators as well as direct indicators for genetic, species and habitat diversity will be assessed for their scientific soundness, practicality, geographic scope and usefulness for stakeholders. Candidate indicators will be tested in a standardised design in twelve case studies across Europe and later in three ICPC countries. Case study regions will include pannonian, alpine, boreal, Atlantic and Mediterranean grassland systems (both organic and/or low-input), rain fed organic farms under temperate and Mediterranean conditions, mixed organic farming, organic special crops and low-input tree/agroforestry systems. Plot, farm and regional scales (where applicable) will be addressed. The investigation will include new agricultural practices, e.g. soil conservation, crop rotation management, seed and crop mixtures and economic issues relating to the costs of indicator measurement and to benefits of biodiversity as perceived by different groups of the population. Stakeholders (farming communities, conservation NGOs, administrators) will be integrated at critical stages of the indicator selection process. A handbook with factsheets will be produced for validated indicators and a sampling design for biodiversity monitoring in organic and low-input farming systems across Europe.

Antova G.A.,Plovdiv University | Stoilova T.D.,Institute of Plant Genetic Resources | Stoilova T.D.,The World Vegetable Center | Ivanova M.M.,Plovdiv University
Journal of Food Composition and Analysis | Year: 2014

The seeds of four accessions of cowpea (Vigna unguiculata L.) from the collection of the Institute of Plant Genetic Resources, Sadovo, Bulgaria were analyzed for their chemical composition and a detailed study of their lipids was carried out. Chemical composition values were as follows: protein content ranged from 22.5 to 25.6%, starch 28.3 to 36.2%, fat 1.3 to 1.9%, insoluble fiber 1.7 to 3.0% and minerals 3.2 to 3.7%. The oil content was relatively low, but an extremely high content of biologically active compounds (tocopherols in the oils range from 3838 to 11,475. mg/kg, phospholipids 12.2 to 27.4%) was noted. In oils from seeds of cowpea palmitic (35.1-47.1%) and linoleic acid (21.7-30.9%) dominated, followed by linolenic (7.3-16.8%) and oleic acid (6.9-10.6%). The main component in sterols composition was stigmasterol (42.1-43.3%), followed by β-sitosterol (27.6-39.5%). In the tocopherol fraction of oils from seeds of cowpea the main component γ-tocopherol varied from 44.0 to 66.6%, followed by δ-tocopherol (30.3-52.8%). Phospholipids in oils and seeds of various accessions of cowpea had similar qualitative and quantitative composition. Phosphatidylcholine was predominant - 34.5-46.0% of total phospholipids. © 2014 Elsevier Inc.

Stoilova T.,Institute of Plant Genetic Resources | Pereira G.,National Plant Breeding Station | Tavares-De-Sousa M.,National Plant Breeding Station
Journal of Central European Agriculture | Year: 2013

There is a long tradition in the cultivation of pulses in the Balkans and the Iberic Peninsula, mainly used for human consumption. Among the major food legumes common bean is the most important. A large range of landraces are still grown that have adaptation to local climatic conditions and resistance or tolerance to diseases and pests allow high yield stability under a low input farming. The main purpose of this work was to analyze the morphological variability of 15 Portuguese and 15 Bulgarian landraces in different environmental conditions (in Bulgaria and Portugal) in order to generate information that can help identifying the most suitable resources with good adaptability to different environments. The landraces were evaluated for 16 morphological characters. A considerable morphological variation was found among genotypes. The majority of landraces had white seeds colour but some had also cream, purple and white with red colours around the hillum. The predominant seed shape was long but three accessions have round shape. The geographical site of the trials (Elvas, Portugal or Sadovo, Bulgaria) determined the cluster pattern of the majority of accessions. In Portugal, the plants required more days to reach the flowering and maturity phases and had higher biological yield. In both environments, the components that mainly determined yield were the number of pods and the number of seeds per plant. The highest value for these traits were found in one Portuguese accession (No. PH2) and one Bulgarian accession (No. 99E0128), in both experimental conditions. © Journal of Central Europian Agriculture 2013.

Stoyanova S.,Institute of Plant Genetic Resources | Boller B.,ART Agroscope Reckenholz Tänikon
Czech Journal of Genetics and Plant Breeding | Year: 2010

The protein diversity of 45 genotypes (forty genotypes selected within two gene pools and five varieties) of meadow fescue was examined. Genetic variation was described using ISTA/UPOV methods for crop variety identification. Modifications of acid-PAGE of alcohol-soluble proteins (prolamins), and SDS-PAGE of salt-soluble proteins (globulins) were elaborated for seed analyses of Festuca pratensis Huds. The results of this study indicated that the genotypes of meadow fescue could effectively be differentiated on the basis of polymorphism, detected between protein patterns. SDS-PAGE presented a higher differentiation power and better repeatability; thus could be used as a rapid and reliable method for the identification of F. pratensis genotypes in breeding programmes and the seed industry.

Popova Z.,Institute of Plant Genetic Resources | Neykov N.,Institute of Plant Genetic Resources
Journal of Central European Agriculture | Year: 2013

The correlations between the yield and the elements which determine it in 10 accesions of durum wheat have been studied. A positive correlation has been established between plant height and the last internode length (r=0.72). It was established that the length of the top /1 st / internode take about 40% of the total stem length. A positive correlation was found between the weight of seeds per plant and the productive tillering (r=0.81). The correlation between number of seeds per spike and weight of seeds per spike, number of spikelets per spike and weight of seeds per spike and the weight of seeds per spike and per plant is - 0.86, 0.83 and 0.76.Moderately the correlations exists between the weight of seeds from the main spike and from the rest stems and correlation between spike length and weight of seeds per spike (r=0.45 and r=0.36). A negative correlation between the weight of seeds per plant and mass of 1000 seeds was established (r=-0.51).

Uzundzhalieva K.S.,Institute of Plant Genetic Resources
Emirates Journal of Food and Agriculture | Year: 2016

The importance of The Sweet Basil (Ocimum basilicum L.) as medical, ornamental and spice plant is well known from ancient times. It`s origin is in Africa and Asia. In the regions with moderate climate are grown more than 150 varieties. The aim of this study is describing and classifying the Basil accessions in the collection of IPGR - Sadovo with the view of their practical use. The experiment is carried out in the experimental field of PGRI - Sadovo during the period 2011-2012. The following biometric indices are reported: Plant - height, width, number of branches, stem - length and diameter; branching first order - length and diameter; leaf - length and width; inflorescence - diameter, height and color; type of inflorescence. Phenological observations were made and the following inter-phase periods are recorded: Sowing, germination, sprouting, blossoming, flowering period, growing season. The results shows that accessions A7E0284, which is with longest flowering and growing periods, very well-leaved and long inflorescence is very suitable for ornamental purposes and in home gardens. Accession A9E0452, which is with maximum number of branches, consequently with maximum leaf mass is most suitable for spice and medical purposes. Hierarchical cluster analysis based on intergroup connections is used to determine the homogeneous groups was made. Thus the accessions are divided into groups (clusters) based on a study of 16 indices. The comparative cluster analysis of Basil accessions in the collection of IPGR - Sadovo shows complete uniformity in the results for the grouping based on morphological parameters and using cluster analysis.

Zhivondov A.,Bulgarian Fruit Growing Institute | Uzundzhalieva K.,Institute of Plant Genetic Resources
Acta Horticulturae | Year: 2012

The paper treats the botanical positions of the new fruit groups of the commercial categories plumcots, pluots and aprium. An appropriate proposal was made for giving concrete taxonomic names and assigning the separate groups of plum-apricot hybrids to concrete systematic ranks. Adopting the results of the analyses by botanists and geneticists will determine and fix the concrete interspecific origin of the hybrids. The phonetically substantiated names of the new botanical species obviously show which of the parent species is positioned as a father parent and which as a mother parent. Thus the plum and the apricot species participating directly in hybridization are specified. It is logical to form the Latin name of each new taxon, rendering an account of the harmonious phonetic sounding, by blending syllables of the mother taxon first, followed by a part of the Latin name of the father taxon, e.g., Armeniaca × Domestica - Armestica.

Spasova U.K.,Institute of plant genetic resources | Todorova P.M.,Higher School Agricultural College
Journal of Central European Agriculture | Year: 2012

The pollen of the wild species from the genus Crocus L., spread in Bulgaria has been analyzed. The investigations, made by light microscope show that the pollen of these species is spherical in shape and round in outlines, comparatively big. These morphological characteristics of the pollen of the wild Bulgarian Crocuses define it as a primitive one [6]. The Scanning Electron Microscope investigations, made by Beug [1], established two types of pollen morphology. The results of our investigation led to the conclusion that the same types are also presented in Bulgarian species: - C.biflorus -type, or syncolpate and C.vernus - type, or inaperturate. Eight of the species belong to the first type and only C. pallasii belongs to the second. The pollen of C. reticulatus is with quite unclear aperture - maybe transition to the inaperturate type.

Stamatov S.,Institute of Plant Genetic Resources | Deshev M.,Institute of Plant Genetic Resources
Bulgarian Journal of Agricultural Science | Year: 2014

Receipt of forms suitable for mechanized harvesting is possible by changing the architecture of the fruit box. It must be resistant to cleavage and seeds to be fastened firmly enough placentas to not fall off the entry into the threshing mechanism. As a result of this research work on a collection of sesame dendrogram was created which identified genetic kinship of the studied forms. Analysis of the results helped to determine the parental pairs in the breeding program. The resulting hybridization forms sesame, which are characterized by cleavage resistant box and attached membrane suitable for mechanized harvesting, with minimum loss of seeds. The architecture of this type of box was saved successfully fixed in the next generations. The study established modes of inheritance of phenological signs responsible for the mechanized harvesting. © 2014 National Centre for Agrarian Sciences. All rights reserved.

Desheva G.,Institute of Plant Genetic Resources | Kyosev B.,Institute of Plant Genetic Resources
Emirates Journal of Food and Agriculture | Year: 2015

The knowledge about of genetic diversity of common winter wheat (Triticum aestivum L.) genotypes is useful for production of more efficient crops adapted to diverse conditions. A set of 32 common winter wheat varieties was used to estimate and then utilize the genetic diversity between common winter wheat genotypes by using cluster analysis and factor analysis and to identify effective factors on genetic improvement. Eight agronomic traits were included in the study. The GCV values were lower than PCV values for all the traits. High genetic advance combined with high heritability showed characters: plant height and spike length. Cluster analysis based on ward's method using Euclidian distance, grouped the cultivars into four clusters. Genotypes in the second group were in the highest rate with respect to number of productive tillers per plant, number of spikelets per spike, number of grains per spike, grain weight per spike and yield per plant. Principal Component analysis was applied to group accessions according to similarity on the basis of eight traits in tree components in the factor plane. The first three components explained 82.63% of total variation in the experiment. Cluster analysis based on the three factors grouped the varieties into four clusters. Genotypes of the first cluster can be used for increase in number of productive tillers per plant in breeding programs. Genotypes of the second cluster can be used for increase in thousand grain weight. Genotypes in the fourth cluster had highest mean with respect to first factor and can be used for increase in number of grain per spike.

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