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Taranova N.A.,RAS A.N. Bach Institute of Biochemistry | Kruhlik A.S.,Institute of Physical Organic Chemistry | Zvereva E.A.,RAS A.N. Bach Institute of Biochemistry | Shmanai V.V.,Institute of Physical Organic Chemistry | And 5 more authors.
International Journal of Analytical Chemistry | Year: 2015

A rapid immunochromatographic assay was developed for the control of tetracycline (TC). The assay is based on the competition between immobilized TC-protein conjugate and TC in a tested sample for binding with polyclonal anti-TC antibodies conjugated to colloidal gold during the flow of the sample along a membrane strip with immobilized reactants. Conjugation of colloidal gold and the total immunoglobulin (IgG) fraction of polyclonal antibodies was used to increase the assay sensitivity to ensure low content of specific antibodies in the conjugate. This allowed effective inhibition of free TC and conjugate binding in the strip test zone. Photometric marker registration allows control of the reduction of binding, thereby enhancing detection sensitivity. The proposed assay allows TC to be detected at concentrations up to 20 ng/mL, exceeding the limit of detection of the known analogues, in a wide working range (more than two orders) of 60 pg/mL to 10 ng/mL, ensured through the use of polyclonal antibodies. The assay time is 10 min. The efficiency of the designed assay is shown to identify TC in milk; the degree of recovery of TC ranges from 90 to 112%. The precision of the concentrations measurements was no more than 10%. © 2015 N. A. Taranova et al.

Pushkarchuk A.L.,Institute of Physical Organic Chemistry | Pushkarchuk A.L.,Institute for Nuclear Problems | Potkin V.I.,Institute of Physical Organic Chemistry | Kilin S.J.,B. I. Stepanov Institute of Physics | And 4 more authors.
Structural Chemistry | Year: 2016

DFT simulations of the electron and spin structure of fullerenol derivatives C60(OH)24·2Fe(C5H4COOH)2 and C60(OH)24·2Ni(C5H4COOH)2 have shown that these compounds form stable complexes with intermolecular hydrogen bonds. Calculated exchange coupling constants for the last complexes indicate that they can possess ferromagnetic properties. If this is true, then compounds will have a great potential for medical application as drug delivers under the control of external magnetic field. © 2015 Springer Science+Business Media New York.

Pokutsa A.,Institute of Physical Organic Chemistry | Kubaj Y.,Institute of Physical Organic Chemistry | Zaborovskyi A.,Institute of Physical Organic Chemistry | Maksym D.,Institute of Physical Organic Chemistry | And 2 more authors.
Applied Catalysis A: General | Year: 2010

A new protocol for the effective oxidation of cyclohexane in acetonitrile at 40 °C and atmospheric pressure into cyclohexanol, cyclohexanone and cyclohexyl hydroperoxide using hydrogen peroxide as the oxidant, vanadyl(IV)-acetylacetonate as the catalyst, oxalic acid and glyoxal as additives is presented with some reaction mechanism proposals. © 2010 Elsevier B.V.

Pokutsa A.,Institute of Physical Organic Chemistry | Zaborovskiy A.,Institute of Physical Organic Chemistry | Maksym D.,Institute of Physical Organic Chemistry | Muzart J.,CNRS Institute of Molecular Chemistry - Reims | And 5 more authors.
Reaction Kinetics, Mechanisms and Catalysis | Year: 2010

The kinetics ofH2O2 cleavage catalyzed by cobalt(II)-acetylacetonate has been studied at 40 °C in water, acetic acid, acetonitrile, tetrahydrofuran, 2-propanol, t-butanol, morpholine, and1,4-dioxane by kinetic and spectroscopic techniques. As revealed, the nature of the solvent has a decisive influence on process. Medium polarizability and polarity have been found to be the rate determining factors. © Akadémiai Kiadó , Budapest, Hungary 2010.

Ryazantsev D.Y.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Tsybulsky D.A.,Institute of Physical Organic Chemistry | Prokhorenko I.A.,RAS Shemyakin Ovchinnikov Institute of Bioorganic Chemistry | Kvach M.V.,Institute of Physical Organic Chemistry | And 5 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

A typical TaqMan™ real-time PCR probe contains a 5'-fluorescent dye and a 3'-quencher. In the course of the amplification, the probe is degraded starting from the 5'- end, thus releasing fluorescent dye. Some fluorophores (including fluorescein) are known to be prone to self-quenching when located near each other. This work is aimed at studying dye-dye and dye-quencher interactions in multiply modified DNA probes. Twenty-one fluorogenic probes containing one and two fluoresceins (FAM), or a FAM-JOE pair, and one or two BHQ1 quenchers were synthesized using non-nucleoside reagents and "click chemistry" post-modification on solid phase and in solution. The probes were tested in real-time PCR using an ~300-bp-long natural DNA fragment as a template. The structural prerequisites for lowering the probe background fluorescence and increasing the end-plateau fluorescence intensity were evaluated and discussed. © Springer-Verlag 2012.

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