Institute of Pharmacy and Technology

Cuttack, India

Institute of Pharmacy and Technology

Cuttack, India
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Parhi R.,Gandhi Institute of Technology and Management | Suresh P.,Institute of Pharmacy and Technology | Patnaik S.,Berhampur University
Journal of Pharmaceutical Investigation | Year: 2015

The aim of the present research was to optimize cryogel formulation of Diltiazem hydrochloride (DZ) with poly(vinyl alcohol) (PVA) and hydroxypropyl methylcellulose (HPMC) by utilizing response surface methodology followed by ex vivo permeation study on the optimized gel containing penetration enhancers. A 3-level factorial design was employed to formulate the experimental runs and to evaluate the effect of two independent variables such as the concentration of PVA and HPMC on characteristics of cryogel such as bioadhesive strength (BS) and in vitro drug release (dependent variables). Response surface plots such as contour and 3D plots were generated by the Design Expert® software to analyze the effect of independent variables on dependent variables. Fourier transform infrared spectroscopy studies confirmed the absence of interaction between DZ and polymers. Among various models generated by the software, quadratic model was found to be best fit for both the responses. Both the formulation factors influenced BS synergistically. However, the effect of HPMC concentration was more pronounced compared to concentration of PVA. But, an opposite effect shown by both the formulation factors on cumulative percentage of drug release (CPR) in 8 h. The optimized batch of cryogel of DZ selected by the software was having composition of 5 % of PVA and 2 % of HPMC. Penetration enhancers such as 1,8-cineole, d-limonene and carvone were incorporated into the optimized gel and permeation study was carried out using abdominal skin of rat. The study demonstrated a highest flux of 118 ± 5.81 µg/cm2/h in case of 1,8-cineole containing gel followed by carvone and d-limonene. © 2015, The Korean Society of Pharmaceutical Sciences and Technology.


Dash S.,Berhampur University | Kanungo S.K.,Institute of Pharmacy and Technology | Dinda S.C.,Mekelle University
International Journal of Pharmacy and Pharmaceutical Sciences | Year: 2014

Objective: The objective of the present investigation was carried out to find the effect of Aponogeton natans Linn. leaf with stalks extracts for its antidiabetic activity in different experimental animal models. Method: The investigation aims on the possible actions of Aponogeton natans Linn. leaf with leaf stalks extracts on glucose homeostasis in normoglycemic rats; Oral Glucose Tolerance Test in rats, screening for antidiabetic activity in alloxan induced diabetic rats (single dose and multidose). Result: Oral administration of the methanol extract at the doses 200 mg/kg body weight exhibited significant antidiabetic activity in different models. It was observed that methanol extract (ANME) of Aponogeton natans Linn. at the dose of 200 mg/kg and Glibenclamide exhibited significant reduction in blood glucose concentration to 84.83±2.23 mg/dl (p≤0.05) and 73.83±1.42 mg/dl (p≤0.01) respectively in comparison to diabetic control group 91.58 mg/dl from 2nd hour. The methanol extract (ANME) (200 mg/kg, p.o.) and standard drug Glibenclamide (2.5 mg/kg, p.o.), significantly decreased the peak blood glucose level to 101.35±9.69 mg/dl and 100.1±9.73 mg/dl (p≤0.05) in comparison to diabetic control group 146.46±9.13 mg/dl after 120th minute of glucose loading. The single administration of methanol extract (ANME) of Aponogeton natans Linn. at the test dose level 200 mg/kg p.o. in diabetic rats showed significant (p≤0.01) reduction in blood glucose level respectively. Methanol extract and Glibenclamide (2.5 mg/kg, p.o.) showed significant reduction in blood glucose level to 212.1±4.1 mg/dl and 210.8±4.3 mg/dl (p≤0.01) respectively to diabetic control 312.6 mg/dl after 2nd hours. The results of the study showed that the methanol extract (ANME) possessed significant antidiabetic activity in the multi dose study. Methanol extract (ANME) of and reference standard Glibenclamide (2.5 mg/ kg, p.o.) decreased blood glucose level significant to 166.66±14.00 and 155±14.88 mg/dl (p≤0.01) respectively in comparision to diabetic control 250.5 mg/dl from the 7th day of the experiment. Conclusion: Hence, present investigation established pharmacological evidences that it can be used as antidiabetic agent. A significant decreases of blood glucose level in single dose multidose study, the methanol extract as compared to control suggests its usefulness in single dose and multidose antidiabetic study.


Parhi R.,Gandhi Institute of Technology and Management | Suresh P.,Institute of Pharmacy and Technology
Journal of Advanced Research | Year: 2016

The present investigation focused on the development of Diltiazem HCl (DTH) matrix film and its characterization by in-vitro, ex-vivo and in-vivo methods. Films were prepared by solvent casting method by taking different ratios of hydroxypropyl methylcellulose K4M (HPMC K4M) and Eudragit RS100. Various parameters of the films were analyzed such as mechanical property using tensile tester, interaction study by Fourier transform infrared spectroscopy (FTIR) and Thermogravimetric analysis (TGA), in-vitro drug release through cellulose acetate membrane, ex-vivo permeation study using abdominal skin of rat employing Franz diffusion cell, and in-vivo antihypertensive activity using rabbit model. The FTIR studies confirmed the absence of interaction between DTH and selected polymers. Thermal analysis showed the shifting of endothermic peak of DTH in film, indicating the dispersion of DTH in molecular form throughout the film. Incorporation of 1,8-cineole showed highest flux (89.7 μg/cm2/h) of DTH compared to other penetration enhancers such as capsaicin, dimethyl sulfoxide (DMSO), and N-methyl pyrrolidone (NMP). Photomicrographs of histology study on optimized formulation (DF9) illustrated disruption of stratum corneum (SC) supporting the ex-vivo results. The in-vivo antihypertensive activity results demonstrated that formulation DF9 was effective in reducing arterial blood pressure in normotensive rabbits. SEM analysis of films kept for stability study (40 ± 2 °C/75% ± 5%RH for 3 months) revealed the formation of drug crystals which may be due to higher temperature. The findings of the study provide a better alternative dosage form of DTH for the effective treatment of hypertension with enhanced patient compliance. © 2015 Production and hosting by Elsevier B.V.


Mishra B.,Institute of Pharmacy and Technology | Kar D.M.,Siksha ‘O’ Anusandhan University | Maharana L.,Siksha ‘O’ Anusandhan University | Mishra G.P.,Biological E. Ltd
Der Pharmacia Lettre | Year: 2016

The objective of the present work is to carry out the physicochemical investigation of powder drug and phytochemical screening of different fractions obtained from hydroalcoholic extract of Solanum torvum (Swartz) fruits. Physicochemical analysis was done to determining total ash, acid insoluble ash, water soluble ash, sulphated ash, extractive value, moisture content, crude fibre content and foaming index. The inorganic elements determination and fluorescence analysis was performed as per the standard methods. Phytochemical investigations were carried out on different fraction to determine various active constituents present in it by using standard preliminary phytochemical tests. The inorganic element, such as magnesium, iron, copper, zinc, manganese and chromium were found in the hydroalcoholic extract of fruits. Florescence analysis of the fractions were reported which gives the sensitivity of the chemical in different chemical reagents. Preliminary phytochemical screening of different fractions of hydroalcoholic extract showed the presence of the phytoconstituents like alkaloids, glycoside, proteins and amino acid, carbohydrates, flavonoids, terpenoids, tannins, saponins and sterols. However the volatile oil, fixed oil and mucilage were found to be absent. The physiochemical examination of the fruits of Solanum torvum Swartz (Dengavegi) can be used as rapid, inexpensive botanical identification and standardization. The phytochemical data obtained from the results of the Solanum torvum fractions may be useful for further pharmacological evaluation.


Parhi R.,Gandhi Institute of Technology and Management | Suresh P.,Institute of Pharmacy and Technology | Pattnaik S.,Berhampur University
Drug Delivery and Translational Research | Year: 2016

In the present study, pluronic lecithin organogel (PLO) of diltiazem hydrochloride (DZH) was developed by taking different ratios of organic phase to aqueous phase (1:3, 1:4, and 1:5) with varying concentration of soya lecithin (20, 30, and 40 % w/w) in organic phase (isopropyl myristate, IPM) and pluronic (20, 25, and 30 % w/w) in aqueous phase, respectively, and characterized for in vitro parameters and ex vivo permeation study. The results of in vitro parameters were found to be within permissible limit and all the PLOs were physically stable at refrigeration and ambient temperature. The influence of phase ratio and different concentrations of soya lecithin on DZH release from the PLOs was found to be significant (p < 0.05), whereas the influences of different concentrations of pluronic were insignificant. The effect of different solvents/penetration enhancers viz. IPM, propylene glycol (PG), dimethyl sulphoxide (DMSO), and d-limonene, in combination and alone, on the permeation of DZH across the dorsal skin of rat was studied. Among all, formulation containing IPM (PLO6) exhibited highest flux of 147.317 μg/cm2/h. Furthermore, histopathology section of treated skin sample illustrated that lipid bilayer disruption was the mechanism for the DZH permeation. The above results indicated that PLO6 may serve as a promising alternative delivery system for DZH in the effective treatment of hypertension. © 2016, Controlled Release Society.


Dash S.,Berhampur University | Kanungo S.K.,Institute of Pharmacy and Technology | Dinda S.C.,Berhampur University
Der Pharmacia Lettre | Year: 2013

The present investigation was carried out to find the effect of Aponogeton natans (Linn.) leaf and leafstalks various extracts for its anti-inflammatory activity in different experimental animal models. The anti-inflammatory activity was evaluated using acute inflammatory models like; carrageenan induced paw edema, histamine induced paw edema and chronic inflammatory model like; cotton-pellet induced granuloma models in rats.Oral administration of the methanolic extract at the doses 200 mg/kg body weight exhibited significant anti-inflammatory activity in different models. In carrageenan and histamine induced paw edema Aponogeton natans (Linn.) methanol extract showed maximum inhibition of 60.89% and 53.97% respectively at late phase. In the cotton pellet induced inflammation study in rats, Aponogeton natans (Linn.) methanol extract 200 mg/kg showed significant decrease in wet weight and dry weight of granuloma tissue formation by 52.72% and 43.76% respectively. Hence, present investigation established pharmacological evidences that it can be used as anti-inflammatory agent. A significant percentage inhibition of paw oedema by the methanol extract as compared to control suggests its usefulness in acute and chronic anti-inflammatory models.


Mishra S.L.,Institute of Pharmacy and Technology | Sinhamahapatra P.K.,Institute of Pharmacy and Technology | Nayak A.,CSIR - Institute of Minerals And Materials Technology | Das R.,CSIR - Institute of Minerals And Materials Technology | Sannigrahi S.,St Peters Institute Of Pharmaceutical Science
Indian Journal of Pharmaceutical Sciences | Year: 2010

The present study evaluated the in vitro antioxidant potential of different parts of Oroxylum indicum. 2,2-diphelyl 1-picrylhydrazyl (DPPH), nitric oxide, superoxide anion and hydroxyl radical scavenging potential and reductive ability assay of methanol extract of different parts i.e. root, root bark, stem, stem bark, leaves and fruits were performed. Leaves and bark extracts exhibits highest free radical scavenging activity than bark, stem and fruit extract. Leaves extract showed maximum reductive ability and found to contain maximum amount of polyphenolic compounds. The highest free radical activity may be due to presence of polyphenolic compounds.


Dash G.K.,Institute of Pharmacy and Technology
International Journal of Pharma and Bio Sciences | Year: 2011

The petroleum ether, chloroform, methanol and aqueous extracts of the leaves of Ageratum conyzoides Linn. (Asteraceae) were evaluated for their wound healing activity in rats using excision (normal and infected), incision and dead space wound models respectively. The effects of test samples on the rate of wound healing were assessed by the rate of wound closure, period of epithelialisation, wound breaking strength, weights of the granulation tissue, determination of hydroxyproline, super oxide dismutase (SOD), catalase and histopathology of the granulation tissues. Nitrofurazone (0.2% w/w) in Simple ointment I. P. was used as reference standard for the activity comparison. The results of the study revealed that the animals treated with methanol and aqueous extracts of A. conyzoides showed faster rate of wound healing compared to other extracts under study. The chloroform extract of the selected plants also produced promising results but the effects are seen to be of lesser extent than the corresponding methanol and aqueous extracts. The petroleum ether extract did not produce significant results. The present work justifies the use of the leaves of A. conyzoides for wound healing activity as claimed in the folklore literature.


Parhi R.,Gandhi Institute of Technology and Management | Suresh P.,Institute of Pharmacy and Technology
Materials Science and Engineering C | Year: 2016

Matrix type of simvastatin transdermal film (SSTF) was developed with poly(vinyl alcohol) (PVA) and eudragit RL100 (EG) using response surface methodology (RSM) to investigate combined effect of the selected process variables like SS concentration, PVA:EG ratio and the dibutyl phthalate (DBT) concentration at different levels on dependent variables such as tensile strength and flux, with an aim to optimize a suitable combination of drug, polymer and plasticizer ratio. The study reveals that the effect of DBT concentration was highest on tensile strength, while SS concentration exhibited pronounced effect on SS flux through the abdominal skin of rat. According to Derringer's desirability prediction tool, the composition of optimized film was found to be 2% of SS, 2:1 ratio of PVA:EG and 40% of DBT. Under these conditions, the SSTF exhibited a predicted value of tensile strength and flux of 11.871 MPS and 43.569 μg/cm2/h, respectively. The in vivo hypolipidemic study conducted for 14 days in hyperlipidemia induced Sprague-Dawley rats reveals that the optimized SSTF exhibited significant lowering of blood lipid level. The absence of skin irritation indicates that the prepared film was safe and well tolerated as transdermal formulation. Thus, the film may serve as an alternative therapy to oral dosage form of SS. © 2015 Elsevier B.V. All rights reserved.


Biswal S.,Institute of Pharmacy and Technology | Sahoo U.,Institute of Pharmacy and Technology | Sethy S.,Institute of Pharmacy and Technology | Kumar H.K.S.,Institute of Pharmacy and Technology | Banerjee M.,Institute of Pharmacy and Technology
Asian Journal of Pharmaceutical and Clinical Research | Year: 2012

A large number of efforts were made to synthesize different heterocyclic compounds and their derivatives in the past decade and were found to posses promising antitumor, anticonvulsant, antimicrobial, anti-tubercular and anti diabetic activities. Although indole moiety is very small but is fascinated by scientists because of the diverse biological activities by not only indole but its various substituted derivatives as well. This review was focused on the indole and its derivatives that are now in development. Due to its wider applications in pharmaceutical industries, they will replace many existing heterocyclic based pharmaceuticals. Now days, many drugs are in the world market, while several hundred are in clinical trials.

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