Aditya Institute of Pharmaceutical science and Research

andhra Pradesh, India

Aditya Institute of Pharmaceutical science and Research

andhra Pradesh, India
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Kumar D.S.,Aditya Institute of Pharmaceutical science and Research | Avasarala H.,Aditya Institute of Pharmaceutical science and Research
Journal of Dietary Supplements | Year: 2013

Objective: The present study evaluated the antihypercholesterolemic effect of Macrotyloma uniflorum (Lam.) Verdc extract by examining its effects on food consumption, weight gain, serum lipid profile, serum glutamate oxaloacetate transamianse (SGOT), serum glutamate pyruvate transaminase (SGPT), and body fat in rats. Methods: Animals were fed with experimental high-fat diets (HFD) that contain 10% lard plus, 5% corn oil. Food intake and body weight were measured. Serum samples were analyzed for the estimation of lipid profiles and other biochemical parameters by using kits. Results: The consumption of ethanol and water extract of experimental plant for 5 weeks caused the decrease in total cholesterol (TC), triglycerides, low-density lipoprotein (LDL), very low density lipoprotein (VLDL) SGOT and SGPT significantly (p < .01), and increase in high-density lipoprotein (HDL) significantly (p < .01). The rat group fed with HFD has shown higher serum TC, TG, LDL, VLDL, SGOT, SGPT, and lower HDL than in the normal control group (p < .01). Fecal excretion of cholesterol in the ethanol extract-treated groups was significantly higher than those treated with water extract. Body weight in the water extract-treated groups was significantly lower than that in the ethanol extract-treated groups. Conclusion: This study demonstrated that M. uniflorum (Lam.) Verdc extracts have strong activities against hypercholesterolemia and obesity suggesting a potential usefulness of M. uniflorum (Lam.) Verdc extracts as functional ingredients. © 2013 Informa Healthcare USA, Inc.


Dinakaran S.K.,Aditya Institute of Pharmaceutical science and Research | Dinakaran S.K.,Jawaharlal Nehru University | Avasarala H.,Aditya Institute of Pharmaceutical science and Research
Journal of Dietary Supplements | Year: 2014

The present study involves the determination of antioxidant capacity and in vitro α-amylase and lipase inhibitory activity of the Crotalaria juncea Linn extract. The content of polyphenols, flavonoids, and tannins in the extracts was estimated by spectrophotometry. Antioxidant activity on goat liver lipid peroxidation and linoleic acid emulsion were determined and α-amylase and lipase inhibitory activity was also evaluated. All the extracts had shown antioxidant property, α-amylase, and lipase inhibitory properties. Aqueous extract was found to show maximum antioxidant activity on goat liver. Antilipid peroxidation and antioxidant activity were determined to be 66.94 ± 0.616 (p < .01) and 59.54 ± 0.2 (p < .01), respectively. Maximum α-amylase and lipase inhibitory activities of 71.42 ± 1.37 (p < .01) and 57.14 ± 2.74% (p < .01), respectively, were exhibited by macerated methanol extract. The results had shown that all the extracts exhibited low inhibition and antioxidant activity as compared to standard. © 2014 Informa Healthcare USA, Inc.


Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Indian Journal of Natural Products and Resources | Year: 2012

An ethnomedicinal survey was carried out in 10 different villages of East Godavari district of Andhra Pradesh with an aim to document the information regarding folkloric uses of indigenous plant species. A total of 32 plant species belonging to 32 genera and 25 families were recorded, and enumerated along with their botanical name, family, local name, part(s) used, ethnomedicinal uses including their method of preparation, mode of administration and dosage. The reported plant species in the form of various traditional preparations are employed by the village people for the management as well as treatment of various ailments ranging from snake bite to fever.


Rudrapal M.,Aditya Institute of Pharmaceutical science and Research
Asian Journal of Chemistry | Year: 2016

A RP-HPLC method was developed and validated for simultaneous determination of minoxidil and aminexil in a combined topical liquid formulation. Isocratic separation was achieved on a C18 column (250 mm × 4.6 mm i.d., particle size 5 μm, ThermoHypersil BDS) using a mobile phase consisting of methanol-phosphate buffer (pH 3.0, 60:40, v/v) at a flow rate of 1 mL/min and UV detection at 250 nm. Linearity was observed over the concentration range of 1.0-2.0 mg/mL for both minoxidil (r2 = 0.99) and aminexil (r2 = 0.99). The average percentage recovery of the method was 101.0 % with a relative standard deviation of 0.14 %. The limits of detection (LODs) were 0.7346 μg/mL and 0.2423 μg/mL for minoxidil and aminexil and limits of quantification (LOQs) were 0.7346 μg/mL and 0.2423 μg/mL, respectively. The method was validated as per ICH guidelines.


Palaksha M.N.,Aditya Institute of Pharmaceutical science and Research | Das S.,Aditya Institute of Pharmaceutical science and Research
Journal of Natural Science, Biology and Medicine | Year: 2010

This study focuses the significant antibacterial activity of Garlic (Allium sativum Linn.) extract on streptomycin-resistant strains solely and in synergism with streptomycin. Gram-positive Staphylococcus aureus ATCC BAA 1026 and gram-negative Escherichia coli ATCC 10536 were made resistant to standard antibiotic streptomycin used as a control in the experiment. Zones of inhibition of different treatment groups were measured by agar-well-diffusion assay and compared with control. Statistical comparison of sole extract and streptomycin synergism with streptomycin control had proved it significant.


PubMed | Jawaharlal Nehru University, Rangaraya Medical College and Aditya Institute of Pharmaceutical science and Research
Type: Journal Article | Journal: Scientia pharmaceutica | Year: 2014

To evaluate the antihypercholesterolemic effects of 50 mg/kg BW and 100 mg/kg BW per day of an ethanolic extract of Crotalaria juncea Linn (whole plant) by performing in vivo studies.The effects of oral administration of 50 mg/kg BW and 100 mg/kg BW per day of an ethanolic extract of Crotalaria juncea Linn (whole plant) in rats fed with a high-fat diet were investigated by evaluating parameters like food consumption, weight gain, fecal fat excretion, serum and liver lipids, and biochemical profiles as well as by histopathological studies. The results were compared to animals fed with the standard diet and animals fed with a high-fat diet and atorvastatin (10 mg/kg BW).The animal group administered with the ethanolic extract for 35 days showed decreased levels of TC, LDL, VLDL, TG, HDL+VLDL, VLDL+LDL, LDL/TC, AI, SGOT, SGPT, and elevated levels of HDL, HDL/TC, significantly (p<0.01 & p<0.05) in a dose-dependent manner. The evaluation of liver tissues of the animal groups treated with the herbal extract and standard had shown increased levels of SOD, GSH, and catalase, whereas levels of SGOT, SGPT, total glucose, HMG-CoA, lipase, amylase, and the percentage of malon-dialdehyde were decreased when compared with the high-fat diet-fed rats. Body weight and food intake in the treated groups were significantly lower than that in the model control.The present study showed that an ethanolic extract of Crotalaria juncea L. influences several blood lipid and metabolic parameters in rats, suggesting a potential benefit as an antihypercholesterolemic agent.


Sarwa K.K.,Dibrugarh University | Rudrapal M.,Aditya Institute of Pharmaceutical science and Research | Debnath M.,DDM College
Biosciences Biotechnology Research Asia | Year: 2013

In the present study, green tea leaves were extracted by four different methods viz. traditional maceration, Soxhlet extraction, ultrasonication extraction and microwave irradiation method, using ethanol as solvent. Methods proposed herein were suitably optimized for the purpose of efficient extraction of tea leaves and further, these methods were subjected to comparative evaluation on the basis of results expressed as percentage yield of air-dried tea extract. From results, ultrasonication and microwave irradiation methods were found to be more efficient in extracting green tea leaves as compared to traditional maceration and conventional Soxhlet methods of extraction.


Sathis Kumar D.,Aditya Institute of Pharmaceutical science and Research | Prashanthi B.D.N.,Aditya Institute of Pharmaceutical science and Research | Harani A.,Andhra University | Anusha P.,Aditya Institute of Pharmaceutical science and Research
Jurnal Teknologi | Year: 2015

Our main objective is to develop an accurate and precise RP-HPLC method for the simultaneous determination of Valacyclovir HCl and Ritonavir in tablet dosage form. An Agilent TC-C18 (2) column is used for the Separation of drugs by a mobile phase consisting of methanol, acetonitrile and water mixture in the ratio of 35:41.5:23.5v/v. The flow rate maintained was 1.3 mL/min and the wavelength used for detection was 222 nm. The linearity was observed in the range of 12.5-125μg/ml for Valacyclovir HCl (VC) and Ritonavir (RT) with a correlation coefficient of 0.9987 and 0.9981 respectively. The mean percentage recoveries for 80%, 100% and 120% accuracy were found to be 101.7%±2.09, 100%±2.49 and 101.5%±1.61 respectively for VC. The mean percentage recoveries for 80%, 100% and 120% accuracy were found to be 104.3%±0.99, 100%±1.77and 99.0%±1.22 respectively for RT. Linearity, accuracy, precision and robustness parameters for the suggested method were estimated for validation. The developed method can be utilized in the analysis of VC and RT tablets. © 2015 Penerbit UTM Press. All rights reserved.


Rudrapal M.,Aditya Institute of Pharmaceutical science and Research | Chetia D.,Dibrugarh University
Pharma Times | Year: 2014

Cancer is a serious health problem affecting people of all ages around the world. Today, the high incidence risk of cancer is mainly attributed to globalization of unhealthy lifestyles; particularly cigarette smoking, inadequate diet like reduced intake of certain edible fruits and vegetables, adopting western modern diet rich in high fat and low fiber content and presence of certain carcinogenic chemicals in foods etc. Recent research has revealed that regular consumption of certain fruits and vegetables can reduce the risk of developing malignant cancers. In this context, dietary phytochemicals (non-nutritive components in the plant-based diet) are considered as potential alternative sources of safer chemicals which possess cancer preventive properties. Such dietary phytochemicals have been known to possess at various degrees their individual chemo-preventive potential against different malignant forms of cancers. These are capsaicin (chili pepper), curcumin (turmeric), diallylsulfide (garlic), epigallocatechin-3-gallate (green tea), genistein (soyabeans), 6-gingerol (ginger), indole-3-carbinol (cabbage), resveratrol (grapes); just to name a few. The chemoprevention of cancer by these edible phytochemicals remains to be a cost-effective, readily accessible and therapeutically effective approach for the better management of cancers in general public. © 2014, Indian Pharmaceutical Association. All rights reserved.


Rao R.N.,Indian Institute of Chemical Technology | Prasad K.G.,Indian Institute of Chemical Technology | Kumar K.V.S.,Aditya Institute of Pharmaceutical science and Research | Ramesh B.,Indian Institute of Chemical Technology
Analytical Methods | Year: 2013

A rapid, sensitive and selective bio-analytical method was developed for determination of elvitegravir and ritonavir in rat plasma by high-performance liquid chromatography-tandem mass spectrometry. Sample preparation was based on diatomaceous earth supported liquid extraction using dichloromethane to extract elvitegravir and ritonavir from rat plasma. Chromatographic separation was performed on a Waters Symmetry C18 column (4.6 × 250 mm, 5 μm) using 20 mM ammonium formate containing 0.05% trifluoroacetic acid and acetonitrile (35:65 v/v) for isocratic elution and mass detection. Calibration curves were linear with regression coefficients greater than 0.997 over the concentration range of 2-5000 ng mL-1 for elvitegravir and ritonavir. Absolute mean recoveries were in the range of 87.31 to 92.18% and no significant interferences were observed at the retention times of the elvitegravir, ritonavir and internal standard. The method exhibited good intra- and inter-day performance in terms of 1.55-7.47% precision and 0-5% accuracy. The developed method has been successfully applied to the pharmacokinetic study of elvitegravir in healthy rats following a single oral administration of a 15 mg kg-1 dose boosted with 5 mg kg-1 ritonavir. © 2013 The Royal Society of Chemistry.

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