Bad Münster am Stein-Ebernburg, Germany
Bad Münster am Stein-Ebernburg, Germany

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Wiesneth S.,Universitatsstr 31 | Petereit F.,Institute of Pharmaceutical Biology and Phytochemistry IPBP | Jurgenliemk G.,Universitatsstr 31
Molecules | Year: 2015

In the present study, a qualitative analysis of proanthocyanidins (PAs) from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1), B2 (2), B3 (3), B4 (4), C1 (5), epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (6) and epicatechin-(4β→8)-epicatechin-(4β→8)-epicatechin-(4β→8)-catechin (7) have been isolated by a combination of different chromatographic separations on Sephadex® LH-20-, MCI®-, Diol-and RP-18-phases. Mass spectrometry, 1D- and 2D-NMR, circular dichroism and polarimetry were used for their structure elucidation and verification by comparison with the literature. Additionally, two fractions of very polar flavan-3-ols were compared: "regular" polymeric PAs received at the very end of the Sephadex® LH-20 chromatography showing no mobility on silica TLC and "unusual" PAs with the same RF-value but already eluting together with flavonoids in the Sephadex® LH-20 system. These "unusual" PAs were subsequently enriched by centrifugal partition chromatography (CPC). C-NMR, polarimetry, thiolysis, acid hydrolysis and phloroglucinol degradation were used to characterize both fractions. Differences in the composition of different flavan-3-ol units and the middle chain length were observed. © 2015 by the authors.


PubMed | Universitatsstr 31 and Institute of Pharmaceutical Biology and Phytochemistry IPBP
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2015

In the present study, a qualitative analysis of proanthocyanidins (PAs) from an aqueous-methanolic extract of Salix daphnoides VILL. bark is described. Procyanidin B1 (1), B2 (2), B3 (3), B4 (4), C1 (5), epicatechin-(48)-epicatechin-(48)-catechin (6) and epicatechin-(48)-epicatechin-(48)-epicatechin-(48)-catechin (7) have been isolated by a combination of different chromatographic separations on Sephadex LH-20-, MCI-, Diol-and RP-18-phases. Mass spectrometry, 1D- and 2D-NMR, circular dichroism and polarimetry were used for their structure elucidation and verification by comparison with the literature. Additionally, two fractions of very polar flavan-3-ols were compared: regular polymeric PAs received at the very end of the Sephadex LH-20 chromatography showing no mobility on silica TLC and unusual PAs with the same RF-value but already eluting together with flavonoids in the Sephadex LH-20 system. These unusual PAs were subsequently enriched by centrifugal partition chromatography (CPC). 13C-NMR, polarimetry, thiolysis, acid hydrolysis and phloroglucinol degradation were used to characterize both fractions. Differences in the composition of different flavan-3-ol units and the middle chain length were observed.

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