Time filter

Source Type

Liestal, Switzerland

Kser S.A.,University of Basel | Fankhauser G.,Hospital of Tafers | Willi N.,Cantonal Institute of Pathology | Maurer C.A.,University of Basel
Scandinavian Journal of Gastroenterology

Objective. Very recently it has been shown that hyperbilirubinemia is a specific predictor of perforation in acute appendicitis. We compared the diagnostic importance of bilirubin, C-reactive protein (CRP), leukocyte count and age as markers of perforation in acute appendicitis. Material and methods. A two-center retrospective cohort study was completed. Patients with acute appendicitis (n = 725) were divided into two groups, group A with perforation (n = 155) and group B without (n = 570). Results. In group A an elevated CRP (>5 mg/l) was measured in 98% of cases versus 72.5% in group B. Hyperbilirubinemia (>20 μmol/l) was measured in 38% of cases in group A versus 22.3% in group B. Leukocytosis (>10 × 10 9/l) was measured in 85% of cases in group A versus 79.3% in group B. Analysis of qualitative and quantitative data showed every marker to be significantly correlated with perforation except elevated white cell blood count. However CRP showed the strongest correlation. The logistic regression model showed CRP to be by far the most significant marker of perforation. Conclusions. Our results confirm hyperbilirubinemia to be a statistically significant marker of perforation in acute appendicitis. However, CRP is superior to bilirubin for anticipation of perforation in acute appendicitis. © 2010 Informa UK Ltd. Source

Bassotti G.,University of Perugia | Villanacci V.,University of Brescia | Salerni B.,University of Brescia | Maurer C.A.,Liestal Hospital | Cathomas G.,Cantonal Institute of Pathology
Techniques in Coloproctology

Chronic constipation requiring surgical ablation for intractability is often a frustrating condition from the pathologist's point of view. In fact, limiting the histological examination to only hematoxylin-eosin staining usually yields only the information that there are no abnormalities. By employing some simple and widely available immunohistochemical methods, discussed in this review, it is possible to gather data that may help in explaining the pathophysiological basis of constipation in these patients. © 2011 Springer-Verlag. Source

Cathomas G.,Cantonal Institute of Pathology | Tarr P.E.,University of Basel
Journal of Radiology Case Reports

Pylephlebitis - suppurative thrombophlebitis of the portal and/or mesenteric veins - is a rare complication of abdominal infections, especially diverticulitis. It can lead to severe complications such as hepatic abscess, sepsis, peritonitis, bowel ischemia, etc., which increase the mortality rate. Here we present a case of suppurative thrombophlebitis of the inferior mesenteric vein, as a complication of sigmoid diverticulitis. The epidemiology, clinical and radiological features as well as treatment strategies are discussed. We also review the anatomy of the mesenteric vein given its anatomic variation in the present case and how this anatomic knowledge might influence the operative approach should surgery be necessary. Source

Bassotti G.,University of Perugia | Villanacci V.,University of Brescia | Nascimbeni R.,University of Brescia | Cadei M.,University of Brescia | And 5 more authors.
Alimentary Pharmacology and Therapeutics

Aliment Pharmacol Ther 2011; 34: 92-99 Summary Background There is recent evidence that mast cells may play important roles in the gut, especially concerning visceral hypersensitivity and motor activity. However, most data are only available for clinical conditions characterised by diarrhoea, where MC have chiefly investigated in the mucosal layer of the colon and there is almost no information concerning constipation. Aim To investigate mast cells distribution in all colonic layers in controls and severely constipated patients. Methods Full-thickness specimens from colons of patients undergoing surgery for slow transit constipation (n = 29), compared with controls, were obtained and the number of mast cells (evaluated by specific monoclonal antibodies) counted as a whole and in single colonic segments (caecum, ascending, transverse, descending and sigmoid). Results Compared with controls, constipated patients revealed significantly higher number of mast cells, both as overall number and in single colonic segments. The distribution of mast cells resulted fairly homogeneous in the various segment of the large bowel, in both controls and patients, and no significant difference in the percentage of degranulated cells was found between groups. Conclusions Colonic mast cells display a homogeneous distribution within the viscus. This cell population is shown to increase in severely constipated patients, which might represent a mechanism trying to compensate for the impaired propulsive activity of these patients. © 2011 Blackwell Publishing Ltd. Source

Obermann E.C.,University of Basel | Mueller N.,University of Basel | Rufle A.,University of Basel | Menter T.,University of Basel | And 4 more authors.
Clinical Cancer Research

Purpose: We investigated whether the first and all subsequent manifestations of Hodgkin lymphoma (HL) in a patient are clonally related. Experimental Design: We identified a collective of 20 patients with sometimes multiple HL recurrences. Relapses were classified as early, that is, within twelve months (eight events in seven patients) or as late, that is, later than one year after the previous neoplasm (24 events in 17 patients). Hodgkin and Reed - Sternberg cells were microdissected after CD30 staining using laser capture technique. Immunoglobulin heavy chain (IgH) gene fragment lengths were analyzed after DNA preamplification, applying consensus FR3 and J primers by ABI 310 Genetic Analyzer. Sequencing of the amplified IgH products was carried out by ABI 3130 and 3730XL Genetic Analyzer. Epstein-Barr virus (EBV) association was assessed by EBV early RNA and LMP1. Results: Three cases with early relapses after a first HL diagnosis were clonally related to the initial tumor, whereas three of four patients with early relapses after a first or second relapse were not, which was accompanied by change of EBV association in one case. Six patients presenting with late relapses were clonally unrelated, which was accompanied by change of phenotype in two cases and change of EBV association in one case. Two samples from recurrent tumors of the same patient could be successfully sequenced. These two late relapses were clonally unrelated by IgH fragment length and sequencing analysis. Conclusions: Recurrent HL, especially those accompanied by an EBV-association switch or after a relapse, can represent an unrelated novel neoplasm. Our finding might play a role in clinical decision making. ©2011 AACR. Source

Discover hidden collaborations