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Lyu Y.,Capital Institute of Paediatrics | Lyu Y.,Peking University | Lyu Y.,University of Toronto | Ouyang F.,Shanghai JiaoTong University | And 4 more authors.
Public Health | Year: 2013

Objective: To examine the trends in prevalence of overweight and obesity, as well as the impact of stunting on overweight/obesity, among rural children aged <60 months in southeast China between 1998 and 2005. Study design: Data from the population-based Child Health Care Surveillance System (CHCSS) from eight counties in southeast China were used. Overweight and obesity were defined as weight-for-height/length z score >2 and >3 standard deviations (SDs), respectively, and stunting was defined as height/length-for-age z score <-2 SD, in accordance with the 2006 World Health Organization growth standard. Methods: A total of 550,693 clinic visit records of 280,931 children from the CHCSS, collected between 1998 and 2005, were included in the analysis. The age- and sex-adjusted prevalence rates of overweight, obesity and stunting were estimated for each year, and the trends over time were examined. The impact of stunting on overweight/obesity was determined using multiple logistic regression analysis. Results: The prevalence of overweight increased from 3.7% in 1998 to 3.9% in 2005 (P<0.001), but no increase in the prevalence of obesity was observed (0.5% in 1998 and 0.6% in 2005; P>0.05). The prevalence of obesity increased significantly in 2005 compared with 1998 among boys aged ≥24 months, but was similar between 1998 and 2005 for girls aged ≥12 months. The prevalence of stunting decreased from 3.9% in 1998 to 1.6% in 2005 (P<0.001). There was a positive association between stunting and overweight/obesity in both 1998 and 2005, although the association was attenuated in 2005. Conclusion: There was a small increase in the prevalence of overweight among rural preschool children in southeast China from 1998 to 2005. The prevalence of obesity remained stable. However, the prevalence of stunting decreased and its positive influence on overweight/obesity was weaker in 2005. © 2013 The Royal Society for Public Health.

Yao H.L.,Capital Institute of Paediatrics
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] | Year: 2012

To evaluate the effects of lentivirus-delivered short hairpin RNA (shRNA) on CVB3 infection in an animal model by RNA interference technique, we constructed a recombinant lentivirus expressing shRNA-3753 against the viral genome region 3753-3771, then transduced Lenti-sh3753 into mice infected with CVB3. We evaluated the antiviral ability of lenti-sh3753 by cytopathic effect (CPE), viral plaque assay and histological analysis of mice hearts. The results showed that Lenti-sh3753 exhibited a significant protective effect on cell viability and reduction of viral titers in supernatant of cell culture by specific inhibition on viral replication. Lenti-sh3753 also prolonged the mice survival and limited the viral production in mice hearts. These data proposed that Lenti-sh3753 can effectively inhibit CVB3 infection in a coxsackievirus-induced myocarditis model, suggesting its potential role in prevention and therapy of viral diseases.

Yang T.,Shanxi Medical University | Yang L.,Shanxi Medical University | Chai W.,Shanghai JiaoTong University | Li R.,Shanxi Medical University | And 3 more authors.
Protein Expression and Purification | Year: 2011

A phage display single-chain variable fragment (scFv) library against TNFα was constructed using a recombinant phage antibody system (RPAS). The cloned scFv gene was introduced into the phage display vector pCANTAB 5E and expressed in Escherichia coli (E. coli) with a yield of up to 0.15 mg/l of total protein. With the attempt to improve the expression level of TNF-scFv, a strategy was established for subcloning the scFv gene from pCANTAB 5E into the plasmid pBV220. Under the control of a highly efficient tandem P RPL promoter system, scFv production was increased to 30% of total protein as inclusion bodies. After extraction from the cell pellet by sonication, the inclusion bodies were solubilized and denatured in the presence of 8 M urea. Purification of denatured scFv was performed using nickel column chromatography followed by renaturation. The purity and activity of the refolded scFv were confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), Western blotting and by an enzyme-linked immunoabsorbent assay (ELISA). The results reveal that the overall yield of bioactive TNF-scFv from E. coli flask cultures was more than 45 mg/l culture medium and 15 mg/g wet weight cells. The renatured scFv exhibited binding activity similarly to soluble scFv. In conclusion we developed a method to over-express TNF-scFv, which have biological function after purification and renaturation. © 2010 Elsevier Inc. All rights reserved.

Jiang L.,Capital Medical University | Zhang H.,Capital Medical University | Zhang H.,Capital Institute of Paediatrics | Xie J.,Capital Medical University | And 5 more authors.
Eye | Year: 2011

Purpose: To evaluate the use of AccuMap multifocal visual evoked potentials (mfVEP) in visual dysfunction caused by macular diseases. Methods: Forty-eight eyes with known macular diseases underwent AccuMap mfVEP and microperimetry 1 (MP1) assessments. Evaluation of mfVEP abnormality was based on an amplitude deviation probability plot and the AccuMap Severity Index (ASI). Correlation analyses of the mean mfVEP amplitude corresponding to a radius of 2°, 5°, and 10° of the central visual field, minimum angle of resolution best-corrected visual acuity (BCVA), and MP1 mean sensitivity of the corresponding areas were performed. Results: Among the 48 affected eyes, AccuMap mfVEP detected an abnormality of the central visual field in 45 eyes, with a sensitivity of 93.8%. The mean mfVEP amplitudes within a radius of 2°, 5°, and 10° of the central visual field were found to be positively correlated with BCVA (P<0.01 for all groups). The mean amplitudes also positively correlated with the MP1 mean sensitivity value of the corresponding visual field (P<0.01 for all groups). In the group with stable fixation or predominantly central fixation, the mean mfVEP amplitudes did not correlate with the BCVA or the MP1 mean sensitivity value. Regardless of the fixation status, the ASI was found to correlate with both the BCVA and the total MP1 mean defect value. Conclusion: Objective perimetry using AccuMap mfVEP might be applied in the assessment of macular function, with the ASI offering a potentially useful indicator for evaluating macular dysfunction. © 2011 Macmillan Publishers Limited All rights reserved.

Xi B.,Capital Institute of Paediatrics | Xi B.,Peking Union Medical College | Zhang L.,Capital Institute of Paediatrics | Mi J.,Capital Institute of Paediatrics
Biomedical and Environmental Sciences | Year: 2010

Objective To explore the metabolic syndrome and its association with arterial compliance in Chinese children and adolescents. Methods 337 participants aged 6 to 18 years with males accounted for 55.8% were grouped according to their traits of metablic syndrome. Anthropometry, blood pressure, fasting plasma glucose, insulin and serum lipid profile were measured. Homeostasis model was assessed and insulin resistance (HOMA-IR) index was measured and calculated for estimating individual insulin resistance. Arterial compliance was also measured using digital pulse wave analyzing method (Micro medical, London), and stiffness index was calculated. Results The stiffness index in participants with metablic syndrome was significant higher than that in participants with no riskof metablic syndrome [(7.69±1.63) vs (6.25±0.86) m/s, P<0.01] and stiffness index and HOMA-IR were progressively increased with the increase of traits of metablic syndrom (P for linear trend <0.001). After gender, age, and pubertal development were adjusted, both traits of metablic syndrome and HOMA-IR were correlated positively with stiffness index (both P<0.05). Conclusion The clustering of metablic syndrome was closely associated with risk at increased arterial stiffness in Chinese children and adolescents. It was suggested that arterial compliance assessment of children and adolescents might be an important measure for prevention of cardiovascular diseases. Copyright © 2010 by China CDC.

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