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Fetoni A.R.,Institute of Otolaryngology | Eramo S.L.M.,University Cattolica | Paciello F.,Institute of Otolaryngology | Rolesi R.,Institute of Otolaryngology | And 2 more authors.
Hearing, Balance and Communication | Year: 2015

Objective: Oxidative stress plays a significant role in noise induced hearing loss (NIHL) as it largely participates in the mechanisms that underlie cell death after noise exposure and leads to sensorineural hearing loss. Many antioxidant drugs have been tested to prevent NIHL. Study design: We compared the protective effects of five molecules having antioxidant properties (vitamin E, ferulic acid, active CoQ10, its synthetic analogue - idebenone, and the soluble formulation CoQter) tested in our laboratory. This study has been conducted in a model of acoustic trauma in rats in which the molecules were given intraperitoneally 1 h before and once daily for three days after pure tone noise exposure (10 kHz for 1 h at 100dB SPL).We evaluated their hearing function via electrophysiological measurements at 2, 7 and 21 days and performed morphological studies with scanning electron microscopy and TUNEL assay as a parameter of apoptotic activation. Results: All molecules decreased threshold shift, reaching almost a complete recovery starting from day 7 with a further light recovery at day 21. At this time-point all treatments reached almost 80-90% of protection; however, CoQter and vitamin E were the most effective treatments. A decreasing number of TUNEL-positive nuclei for each treatment were observed two days after noise exposure. The cochleogram and morphological observations were consistent with the protective effects measured by ABR, 21 days after trauma. Conclusion: These results indicate that the best protection can be achieved by using antioxidant molecules acting against mitochondrial induced oxidative stress, such as vitamin E and CoQter and the degree of protection depends on the pharmacological properties of the molecules. © 2015 International Association of Physicians in Audiology.


Yi H.J.,Chinese PLA General Hospital | Yi H.J.,Institute of Otolaryngology | Guo W.,Institute of Otolaryngology | Wu N.,Institute of Otolaryngology | And 5 more authors.
Acta Oto-Laryngologica | Year: 2014

Conclusion: Compared with traditional animal models, the miniature pig may be a better model for biomedical research because its morphology has many similarities with that of humans. Objective: To investigate the suitability of the miniature pig as an animal model for otological research as regards morphology. Methods: Microdissection of the temporal bone of 10 miniature pigs was performed and recorded on photographs. Results: The morphology and measurements of the external, middle, and inner ear, and the lateral recess of the miniature pigs were completed by microdissection. The temporal bone structures, including the external, middle, inner ear, and the lateral recess, were similar in the miniature pig and humans. © Informa Healthcare.


Hu B.,Capital Medical University | Han D.,Capital Medical University | Zhang L.,Capital Medical University | Li Y.,Capital Medical University | And 7 more authors.
American Journal of Rhinology and Allergy | Year: 2010

Background: Olfactory disorders are a common syndrome in the rhinology clinic. Olfactory event-related potential (OERP) has been considered as an important alternative method to evaluate olfactory function. The aim of this study was to find the consistency of OERP and the psychophysics olfactory test (T&T olfactometry assessment) in rhinosinusitis patients with or without nasal polyposis. Methods: Both the psychophysics olfactory test and the OERP were performed in all patients before functional endoscopic sinus surgery (FESS). Detailed information including demographic, comorbidity, subjective symptoms, CT, and endoscopic examination were recorded. Six months later, the T&T test and OERP were reexamined in patients with preoperative identified OERP. Results: Preoperative OERP was identified in 26 patients of the nonpolyp group and 12 patients of the polyp group. Good correlation existed between T&T scores and amplitude and latency of N1 and P2 in 26 nonpolyp patients (p < 0.05). No meaningful correlation was apparent in 12 polyp patients (p > 0.05). Significant correlation existed in 7 polyp patients with a free olfactory cleft (OC) according to CT (p < 0.05). The other 5 patients with partial OC obstruction were anosmic according to the psychophysics olfactory test; however, OERP was present. Six months later, T&T scores decreased significantly only in these five patients (p < 0.05). Significant correlation was obtained between postoperative T&T scores and OERP in both polyp and nonpolyp groups (p < 0.05). Conclusion: OERP can be used to investigate olfactory function of rhinosinusitis patients. Nasal polyp obstruction in specific parts of the OC might influence the consistency between the psychophysics olfactory test and OERP. Copyright © 2010, OceanSide Publications, Inc.


Li J.,Institute of Otolaryngology | Ji F.,Institute of Otolaryngology | Chen W.,Institute of Otolaryngology | Zhao H.,Institute of Otolaryngology | And 2 more authors.
Acta Oto-Laryngologica | Year: 2014

Objective: The aim of this study was to analyze the safety and effectiveness of a new cochlear implant (CI) system developed in China, the Nurotron Venus device. Material and methods: Fifteen post-lingually deafened patients received Nurotron Venus CIs in our hospital. The safety and effectiveness of the devices were evaluated within 2 years after implantation. Patients' hearing thresholds were assessed. In addition, the speech perception performance of Nurotron Venus CI recipients was compared with that of 15 Cochlear Nucleus CI24 recipients. Results and conclusion: During 2 years of observation, all the Nurotron recipients used their devices regularly and effectively. The aided hearing thresholds of all the recipients were within the speech spectrum. The average scores of HOPE sentences and HOPE monosyllable words tests among Nurotron CI recipients were 82.88 ± 21.40% and 56.67 ± 9.77%, respectively. The average scores among Cochlear Nucleus CI24 recipients were 87.33 ± 14.44% and 52.8 ± 12.76%, respectively. There was no statistically significant difference in the speech test scores between these two groups when assessed using the t test. The Nurotron Venus cochlear implant system worked safely and effectively. The speech perception of Nurotron recipients was similar to that of the other CI system recipients. © 2014 Informa Healthcare.


Han D.,Capital Medical University | Han D.,Institute of Otolaryngology | Zhang L.,Capital Medical University | Zhang L.,Institute of Otolaryngology
Acta Oto-Laryngologica | Year: 2011

Nasal cavity ventilation expansion surgery has been progressively developed alongside an advanced understanding of the pathophysiological mechanisms of sleep-disordered breathing and the maturation of endoscopic surgery techniques. Nasal cavity ventilation expansion surgery could increase the ventilation volume of the nasal cavity effectively and decrease the nasal resistance, relieve the upper airway obstruction, and finally remove the original obstructive factor in the upper airway obstruction. The major significance of these procedures is to decrease the pre-ventilation resistance of the upper airway, to relieve pharyngeal collapse, to recover normal ventilatory function, to promote physical recovery, to re-establish normal metabolic functions, and to restore sleep structure. Nasal cavity ventilation expansion surgery includes a series of procedures and to open nasal sinuses symmetrically could decrease the nasal resistance, relieve the upper airway obstruction, and finally remove the original obstructive factor in the upper airway obstruction. The application of endoscopic surgery to obstructive sleep apnea (OSA) enhances the ventilation volume and symmetry of nasal cavity ventilation and has expanded the range of indications for which functional endoscopic sinus surgery (FESS) is effective. Combined with H-uvulopalatopharyngoplasty (H-UPPP), the outcome of surgical treatment on ventilation disorders of the upper airway obstruction and the symptoms of OSA will both be improved. © 2011 Informa Healthcare.


Zhai S.,Institute of Otolaryngology | Chen P.,PLA General Hospital | Guo W.,Institute of Otolaryngology | Yu N.,Institute of Otolaryngology | And 2 more authors.
Acta Oto-Laryngologica | Year: 2010

Conclusion: The results of our research indicate that 125I- labelled basic fibroblast growth factor (125I-bFGF) has some difficulty in getting across the bloodlabyrinth barrier (BLB). Therefore, it is necessary to carry out further study on the route of bFGF administration. Objective: To observe whether bFGF could pass through the BLB after intraperitoneal injection and to establish an experimental basis for its clinical applications. Methods: Thirty guinea pigs were divided into three equal groups. Animals in group 1 were administered 125I-bFGF, while animals in groups 2 and 3 were given 125I or saline solution, respectively, via intraperitoneal injection. The animals were sacrificed 2 h later and samples of blood, brain, cochlea, thyroid gland, heart, liver, and kidney were collected and weighed. A radioimmunoassay analyzer was employed to count the CPM values of each sample, and autoradiography was performed on section samples of both cochleae. Results: The CPM value of organ samples in the 125I group was higher than that in other groups, and radioactive grain was observed in cochlear samples of this group. In the 125I-bFGF group, blood demonstrated the highest CPM value, while cochlea and brain demonstrated the lowest CPM value, with no radioactive grain observed in cochlear samples. © 2010 Informa UK Ltd. (Informa Healthcare, Taylor & Francis As).

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