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Zou S.,Shanghai JiaoTong University | Shen X.,Shanghai University | Tang Y.,Shanghai University | Fu Z.,Organ Transplantation Institute of PLA | And 2 more authors.
Transplantation Proceedings | Year: 2010

The effect of astilbin on acute graft rejection was investigated in C57BL/6 mice carrying BALB/c hearts heterotopically transplanted into the neck vessels. Daily treatment with astilbin (50, 125, or 250 mg/kg intraperitoneally) significantly prolonged the survival of grafts in a dose-dependent manner, when cyclosporine (CsA; 5 mg/kg) was co-administered with astilbin (250 mg/kg), there was more potent immunosuppression than that solely achieved by 20 mg/kg CsA. Addition of 10 mg/mL astilbin significantly inhibited the proliferation and activation of T cells, as determined by 3Hthymidine deoxyribose uptake, Western blots for nuclear factor κB and p38, and 1-way mixed lymphocyte reactions (MLR). Mature and antigen-presenting functions of dendritic cells (DCs) also were inhibited by astilbin (10 mg/mL), as determined by morphologic observations, flow cytometry, and MLR. These observations suggested that astilbin is a potential candidate for immunosuppressive therapy after heart engraftment. Inhibiting the maturation and antigen-presenting function of DCs and thus preventing T-cells activation is a possible mechanism underlying its inhibitory effects on acute heart allograft rejection. © 2010 by Elsevier Inc. All rights reserved.


Song J.-Y.,Organ Transplantation Institute of PLA | Du G.-S.,Organ Transplantation Institute of PLA | Zhu Z.-D.,Organ Transplantation Institute of PLA | Zheng D.-H.,Organ Transplantation Institute of PLA | And 3 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

BACKGROUND: Previous studies have reported the cause and treatment of biliary complication. However, how to improve operative technique for preventing the complication is rarely reported. OBJECTIVE: To explore the effect of operational skills during liver transplantation on biliary complications. METHODS: Biliary complications in 475 patients who underwent liver transplantation were retrospectively analyzed. The relationship between operational skills and biliary complications after liver transplantation was observed. The potential risk factors about operative technique were summarized. Some preventive interventions for biliary complications were suggested. RESULTS AND CONCLUSION: Biliary complication was diagnosed in 36 (7.6%) of 475 patients who underwent liver transplantation. They were nonanastomotic biliary stricture (n=19, 4.0%), anastomotic biliary stricture (n=7, 1.5%), biliary leakage (n=3, 0.6%), twisted common biliary duct (n=3, 0.6%), residual common duct stone (n=1, 0.2%), and neoformative common duct stone (n=3, 0.6%). There was no difference in the incidence of nonanastomotic biliary stricture among the three biliary anastomotic styles. The possibility of anastomotic biliary stricture in placing T-drainage tube group was lower than the other two groups according to clinical data. Nevertheless, there was no statistical difference between these three groups. Infusing UW into the liver from cranial mesenteric vein and douching the biliary duct immediately while taking the donor could decrease the incidence of biliary complication after liver transplantation (P=0.013 and P=0.018, OR=0.26 and OR=0.28), the later factor could also decrease the incidence of nonanastomotic biliary stricture (P=0.001, OR=0.09). Meanwhile, some operational skills also decrease the incidence of biliary complications, such as protecting the artery around the biliary duct, and elevating the liver when suturing the common biliary duct.


Chen C.-Q.,Chinese PLA Medical School | Shi B.-Y.,Organ Transplantation Institute of PLA | Cai M.,Organ Transplantation Institute of PLA | Zhao Y.-B.,Organ Transplantation Institute of PLA | And 6 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014

Background: Cytotoxic T lymphocyte-associated antigen 4 is a newly discovered costimulatory molecule. It has been studied more in tumor and autoimmune diseases, less in the field of kidney transplantation. Objective: To explore the role of cytotoxic T lymphocyte-associated antigen 4 in acute rejection after renal transplantation. Methods: Fifty patients undergoing renal transplantation were divided into acute rejection group (20 cases) and stable graft function group (30 cases). Another 30 healthy persons served as control group. Blood samples were extracted from the peripheral blood. Cytotoxic T lymphocyte-associated antigen 4 was detected by enzyme linked immunosorbent assay and flow cytometry. Results and Conclusion: The expression of cytotoxic T lymphocyte-associated antigen 4 in the serum showed significant differences in the acute rejection group, stable graft function group and healthy control group (F=70.008 1, P=0.000 0), but showed no difference in peripheral blood lymphocytes of three groups (F=1.865 6, P=0.161 7). Compared with the healthy control group, the expression levels of cytotoxic T lymphocyte-associated antigen 4 in peripheral blood lymphocytes of acute rejection group and stable graft function group were significantly decreased (P=0.000 0). In addition, the acute rejection group had a lower cytotoxic T lymphocyte-associated antigen 4 expression than the stable graft function group (P=0.000 0). In renal transplant rejection, the expression of cytotoxic T lymphocyte-associated antigen 4 in serum was reduced, showing some correlation with acute rejection after renal transplnatation. Cytotoxic T lymphocyte-associated antigen 4 might be involved in the rejection.


Shi B.,Organ Transplantation Institute of PLA | Yuan Q.,Organ Transplantation Institute of PLA
Medical Journal of Wuhan University | Year: 2016

In this paper, we review the progress on the organ transplantation immune tolerance especially on the transplantation immune tolerance induction mechanism, the design of induction and immune tolerance markers, to provide theoretical support for clinical immune tolerance. © 2016, Editorial Board of Medical Journal of Wuhan University. All right reserved.

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