Xiao G.,Institute of Oil Crops National Oil Crops Improvement Center |
Xiao G.,Pre State Key Laboratory for Germplasm Innovation and Resource Utilization of Crops |
Zhang Z.-Q.,Institute of Oil Crops National Oil Crops Improvement Center |
Wu X.-M.,Institute of Oil Crops National Oil Crops Improvement Center |
And 3 more authors.
Acta Agronomica Sinica | Year: 2010
FAD2 gene is essential for converting oleic acid to linoleic acid in plant seeds. From Xiangyou 15, a cultivar of Brassica napus, 56 FAD2 DNA clones and 47 FAD2 cDNA clones were obtained including 6 new copies of FAD2, which were designated FAD2P1 to FAD2P6. These new FAD2 copies ranged from 1141 bp to 1157 bp in length and contained no introns in their open reading frames. They had 96.1% homology in nucleotide sequence and shared more than 87.0% of nucleotides identity to the published FAD2 gene (AY577313). The deduced amino acid sequences of the 6 FAD2 copies presented 1-12 stop codons within the coding regions, which prevented from coding functional proteins. These copies were transferred into Saccharomyces cerevisiae through vector pYES2.0, and the expression products showed no functions to synthesize 16:2 and 18:2 fatty acids. These results indicate that they are pseudogenes of FAD2. Copyright © 2010, Crop Science Society of China and Institute of Crop Sciences, Chinese Academy of Agricultural Sciences.