Institute of Occupational Medicine and Environmental Health in Sosnowiec

Sosnowiec, Poland

Institute of Occupational Medicine and Environmental Health in Sosnowiec

Sosnowiec, Poland
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Dobrakowski M.,Medical University of Silesia, Katowice | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Kasperczyk A.,Medical University of Silesia, Katowice | Kozlowska A.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | And 3 more authors.
Human and Experimental Toxicology | Year: 2017

There are many discrepancies among the results of studies on the genotoxicity of lead. The aim of the study was to explore lead-induced DNA damage, including oxidative damage, in relation to oxidative stress intensity parameters and the antioxidant defense system in human leukocytes. The study population consisted of 100 male workers exposed to lead. According to the blood lead (PbB) levels, they were divided into the following three subgroups: a group with PbB of 20-35 μg/dL (low exposure to lead (LE) group), a group with a PbB of 35-50 μg/dL (medium exposure to lead (ME) group), and a group with a PbB of >50 μg/dL (high exposure to lead (HE) group). The control group consisted of 42 healthy males environmentally exposed to lead (PbB < 10 μg/dL). A comet assay was used to measure the DNA damage in leukocytes. We measured the activity of superoxide dismutase (SOD), catalase, glutathione reductase (GR), glucose-6-phosphate dehydrogenase (G6PD), and glutathione-S-transferase (GST) as well as the concentration of malondialdehyde (MDA), and the value of the total antioxidant capacity. The level of PbB was significantly higher in the examined subgroups than in the control group. The percentage of DNA in the tail was significantly higher in the LE, ME, and HE subgroups than in the control group by 10% (p = 0.001), 15% (p < 0.001), and 20% (p < 0.001), respectively. The activity of GR was significantly lower in the LE and ME subgroups than in the control group by 25% (p = 0.007) and 17% (p = 0.028), respectively. The activity of G6PD was significantly lower in the ME subgroup by 25% (p = 0.022), whereas the activity of GST was significantly higher in the HE subgroup by 101% (p = 0.001) than in the control group. Similarly, the activity of SOD was significantly higher in the LE and ME subgroups by 48% (p = 0.026) and 34% (p = 0.002), respectively. The concentration of MDA was significantly higher in the LE, ME, and HE subgroups than in the control group by 43% (p = 0.016), 57% (p < 0.001), and 108% (p < 0.001), respectively. Occupational lead exposure induces DNA damage, including oxidative damage, in human leukocytes. The increase in DNA damage was accompanied by an elevated intensity of oxidative stress. © SAGE Publications.


Dobrakowski M.,University of Silesia | Boron M.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Czuba Z.P.,University of Silesia | Birkner E.,University of Silesia | And 3 more authors.
Toxicology and Applied Pharmacology | Year: 2016

The aim of the study was to investigate the influence of a short-term exposure to lead on the blood morphology and the levels of selected cytokines related to hematopoiesis in occupationally exposed workers.The study population included 37 males occupationally exposed to lead for 36 to 44 days. Their blood lead level raised from 10.7 ± 7.67 μg/dl at baseline to the level of 49.1 ± 14.1 μg/dl at the end of the study.The level of hemoglobin and values of MCH and MCHC were decreased due to a short-term exposure to lead by 2%, 2%, and 1%, respectively. The counts of WBC, LYM, and MXD increased significantly by 5%, 7%, and 35%. Similarly, the count of PLT increased by 7%, while PDW, MPV, and P-LCR decreased by 6%, 3%, and 9%, respectively. The levels of IL-7, G-CSF, HGF, PDGF AB/BB, SCF, and PECAM-1, decreased significantly by 30%, 33%, 8%, 30%, 25%, and 20%, respectively.A short-term occupational exposure to lead results in a decreased hemoglobin level and increased counts of WBC and PLT. Changes in counts and proportions of different types of leukocytes and decreased values of PLT indices, such as PDW, MPV, and P-LCR, due to the subacute lead-exposure may be associated with lead-induced decreased levels of cytokines related to hematopoiesis, including SCF, G-CSF, IL-7, and PDGF. © 2016 Elsevier Inc.


PubMed | Independent Public Health Care Center in Staszow, Medical University of Silesia, Katowice and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: | Journal: Human & experimental toxicology | Year: 2016

There are no common recommendations regarding electrocardiographic monitoring in occupationally exposed workers. Therefore, the present study was designed to investigate whether exposure to lead results in an increase of selected electrocardiography (ECG) pathologies, such as QT interval prolongation and repolarization disorders, in occupationally exposed workers. The study group included 180 workers occupationally exposed to lead compounds. The exposed group was divided according to the median of the mean blood lead level (PbB


PubMed | University of Silesia and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: | Journal: Environmental toxicology and pharmacology | Year: 2016

The aim of the study was to investigate the influence of subacute exposure to lead on the glutathione-related antioxidant defense and oxidative stress parameters in 36 males occupationally exposed to lead for 403.2days. Blood lead level in the examined population increased significantly by 359% due to lead exposure. Simultaneously, erythrocyte glutathione level decreased by 16%, whereas the activity of glutathione-6-phosphate dehydrogenase in erythrocytes and leukocytes decreased by 28% and 10%, respectively. Similarly, the activity of glutathione-S-transferase in erythrocytes decreased by 45%. However, the activity of glutathione reductase in erythrocytes and leukocytes increased by 26% and 6%, respectively, whereas the total oxidant status value in leukocytes increased by 37%. Subacute exposure to lead results in glutathione pool depletion and accumulation of lipid peroxidation products; however, it does not cause DNA damage. Besides, subacute exposure to lead modifies the activity of glutathione-related enzymes.


PubMed | University of Silesia, Institute of Occupational Medicine and Environmental Health in Sosnowiec and SP ZOZ Municipal Hospital
Type: | Journal: Toxicology and applied pharmacology | Year: 2016

The aim of the study was to investigate the influence of a short-term exposure to lead on the blood morphology and the levels of selected cytokines related to hematopoiesis in occupationally exposed workers. The study population included 37 males occupationally exposed to lead for 36 to 44days. Their blood lead level raised from 10.77.67g/dl at baseline to the level of 49.114.1g/dl at the end of the study. The level of hemoglobin and values of MCH and MCHC were decreased due to a short-term exposure to lead by 2%, 2%, and 1%, respectively. The counts of WBC, LYM, and MXD increased significantly by 5%, 7%, and 35%. Similarly, the count of PLT increased by 7%, while PDW, MPV, and P-LCR decreased by 6%, 3%, and 9%, respectively. The levels of IL-7, G-CSF, HGF, PDGF AB/BB, SCF, and PECAM-1, decreased significantly by 30%, 33%, 8%, 30%, 25%, and 20%, respectively. A short-term occupational exposure to lead results in a decreased hemoglobin level and increased counts of WBC and PLT. Changes in counts and proportions of different types of leukocytes and decreased values of PLT indices, such as PDW, MPV, and P-LCR, due to the subacute lead-exposure may be associated with lead-induced decreased levels of cytokines related to hematopoiesis, including SCF, G-CSF, IL-7, and PDGF.


PubMed | University of Silesia and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: Journal Article | Journal: International archives of occupational and environmental health | Year: 2016

This study aimed to compare the influence of lead on the non-enzymatic antioxidant defenses and the levels of chemokines in workers subchronically and chronically exposed to lead.The study population was divided into three groups. The first group consisted of male workers subchronically exposed to lead for 403.2days, while the second group included male workers chronically exposed to lead. The third group was a control group.The levels of uric acid and bilirubin were significantly higher after a subchronic exposure to lead compared to the baseline by 22 and 35%, respectively. Similarly, the values of total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) increased by 15, 50, and 33%, respectively. At the same time, the levels of thiol groups and albumin decreased by 5 and 8%, respectively. Additionally, the levels of interleukin-8 (IL-8) and macrophage inflammatory protein-1 (MIP-1) were significantly higher after a subchronic exposure to lead compared to the baseline by 34 and 20%, respectively. Moreover, IL-8 level was significantly higher by 40% in the group of workers chronically exposed to lead than in the control group, while the level of interferon gamma-induced protein-10 (IP-10) was significantly lower by 28%.Similar to chronic lead exposure, subchronic exposure to lead is associated with elevated blood levels of uric acid and bilirubin in humans. This probably results in increased TAC value despite thiol depletion. However, the compensatory activation of non-enzymatic antioxidant defenses seems to be insufficient to protect against lead-induced oxidative stress, which may be additively enhanced by the pro-inflammatory action of chemokines, especially IL-8.


Gac P.,Wroclaw Medical University | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Poreba R.,Wroclaw Medical University | Poreba M.,Wroclaw Medical University | And 3 more authors.
Environmental Toxicology and Pharmacology | Year: 2012

Purpose: Present study aimed at determining blood selenium concentration (Se-B) in a selected population of children inhabiting industrial regions. Methods: The studieswere conducted on a group of 267 children inhabiting industrial regions in Upper Silesia (Poland). Determination of Se-B was performed using hydride generation atomic absorption technique. Results: In the studied group of children mean Se-B amounted to 76.75±12.52 μg/L. Only in 38.20% of studied children the estimated Se-B could be regarded as an optimal. Children with underweight (BMI < 15th centile) manifested a significantly lower Se-B than children with BMI within the normal range (BMI 15-85th centile). In the entire study group of children a significant positive linear correlation was disclosed between BMI and Se-B (r = 0.16, p < 0.05). Regression analysis demonstrated that in the studied subgroup of children (with normal height, body mass and BMI) a higher BMI represented an independent factor of Se-B increase and a more pronounced age an independent factor of Se-B decrease. Moreover, maternal education represented an independent factor of Se-B increase in the studied group of children (with normal height, body mass and BMI). Conclusions: Se-B in the studied group children from industrial area in Upper Silesia corresponded with the lower range of blood selenium concentrations noted in recent years in other populations. A negative relationship was documented between age and Se-B and a positive relationship between BMI of a child and maternal education on one hand and Se-B of a child on the other. © 2012 Elsevier B.V.


PubMed | Wroclaw Medical University and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: Journal Article | Journal: Environmental toxicology and pharmacology | Year: 2015

The study aimed at defining the relationship between blood selenium concentration (Se-B) and levels of oxidative stress and antioxidative capacity in healthy children. The studies were conducted on 337 children (mean age: 8.531.92 years). The groups of individuals with Se-B <1st quartile (group I, Se-B<70g/L), with Se-B fitting the range of 1st quartile and median (group II, Se-B: 70-76.9g/L), with Se-B between the median and 3rd quartile (group III, Se-B: 77-83.9g/L) and those with Se-B above the 3rd quartile (group IV, Se-B84g/L) were distinguished. Level of oxidative stress was defined using determination of urine malonyldialdehyde concentration (MDA) and urine 8-hydroxy-2-deoxyguanosine concentration (8-OHdg). Urine total antioxidant status (TAS) was determined. In group IV TAS was significantly higher than in groups I-III. A positive correlation was detected between Se-B and TAS. In healthy children an appropriately high Se-B seems to ensure higher total antioxidative status.


PubMed | Wroclaw Medical University and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: Journal Article | Journal: Biological trace element research | Year: 2016

This study aimed at evaluation of a relationship between blood selenium concentration (Se-B) and blood cystatin C concentration (CST) in a randomly selected population of healthy children, environmentally exposed to lead and cadmium. The studies were conducted on 172 randomly selected children (7.980.97years). Among participants, the subgroups were distinguished, manifesting marginally low blood selenium concentration (Se-B 40-59g/l), suboptimal blood selenium concentration (Se-B: 60-79g/l) or optimal blood selenium concentration (Se-B80g/l). At the subsequent stage, analogous subgroups of participants were selected separately in groups of children with BMI below median value (BMI <16.48kg/m


PubMed | Medical University of Silesia, Katowice and Institute of Occupational Medicine and Environmental Health in Sosnowiec
Type: Journal Article | Journal: Toxicology and industrial health | Year: 2016

N-Acetylcysteine (NAC) could be included in protocols designed for the treatment of lead toxicity. Therefore, in this study, we decided to investigate the influence of NAC administration on homocysteine (Hcy) levels, oxidative damage to proteins, and the levels of iron (Fe), transferrin (TRF), and haptoglobin (HPG) in lead (Pb)-exposed workers. The examined population (n = 171) was composed of male employees who worked with Pb. They were randomized into four groups. Workers who were not administered any antioxidants, drugs, vitamins, or dietary supplements were classified as the reference group (n = 49). The remaining three groups consisted of workers who were treated orally with NAC at three different doses (1 200, 2 200, or 2 400 mg) for 12 weeks. After the treatment, blood Pb levels significantly decreased in the groups receiving NAC compared with the reference group. The protein concentration was not affected by NAC administration. In contrast, Hcy levels significantly decreased or showed a strong tendency toward lower values depending on the NAC dose. Levels of the protein carbonyl groups were significantly decreased in all of the groups receiving NAC. Conversely, glutamate dehydrogenase activity was significantly elevated in all of the groups receiving NAC, while the level of protein thiol groups was significantly elevated only in the group receiving 200 mg of NAC. Treatment with NAC did not significantly affect Fe and TRF levels, whereas HPG levels showed a tendency toward lower values. Treatment with NAC normalized the level of Hcy and decreased oxidative stress as measured by the protein carbonyl content; this effect occurred in a dose-dependent manner. Moreover, small doses of NAC elevated the levels of protein thiol groups. Therefore, NAC could be introduced as an alternative therapy for chronic Pb toxicity in humans.

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