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Kasperczyk S.,Medical University of Silesia, Katowice | Dobrakowski M.,Medical University of Silesia, Katowice | Kasperczyk A.,Medical University of Silesia, Katowice | Boron M.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Birkner E.,Medical University of Silesia, Katowice
Environmental Toxicology and Pharmacology | Year: 2016

The aim of the study was to investigate whether α-tocopherol supplementation for workers who are chronically exposed to lead would normalize/improve the values of parameters that are associated with the lead-induced oxidative stress.Study population included chronically lead exposed males who were divided into two groups. Workers in the first group (reference group) were not given any antioxidants, while workers in the second group (αT group) received supplementation with α-tocopherol.After treatment, the blood lead and leukocyte malondialdehyde levels decreased significantly in the αT group compared to the baseline levels and reference group. However, the erythrocyte malondialdehyde, conjugated dienes, and lipofuscin levels significantly increased compared to the baseline levels. The glutathione level significantly increased compared with the baseline.Effects of supplementation with α-tocopherol on oxidative damage were not satisfactory. Therefore, there is no reason to administer α-tocopherol to workers chronically exposed to lead as a prophylaxis of lead poisoning. © 2016 Elsevier B.V. Source


Gac P.,Wroclaw Medical University | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Poreba R.,Wroclaw Medical University | Poreba M.,Wroclaw Medical University | And 5 more authors.
Environmental Toxicology and Pharmacology | Year: 2015

The study aimed at defining the relationship between blood selenium concentration (Se-B) and levels of oxidative stress and antioxidative capacity in healthy children. The studies were conducted on 337 children (mean age: 8.53 ± 1.92 years). The groups of individuals with Se-B <1st quartile (group I, Se-B. <. 70. μg/L), with Se-B fitting the range of 1st quartile and median (group II, Se-B: 70-76.9. μg/L), with Se-B between the median and 3rd quartile (group III, Se-B: 77-83.9. μg/L) and those with Se-B above the 3rd quartile (group IV, Se-B. ≥. 84. μg/L) were distinguished. Level of oxidative stress was defined using determination of urine malonyldialdehyde concentration (MDA) and urine 8-hydroxy-2-deoxyguanosine concentration (8-OHdg). Urine total antioxidant status (TAS) was determined. In group IV TAS was significantly higher than in groups I-III. A positive correlation was detected between Se-B and TAS. In healthy children an appropriately high Se-B seems to ensure higher total antioxidative status. © 2014 Elsevier B.V. Source


Dobrakowski M.,University of Silesia | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Hudziec E.,University of Silesia | Kozlowska A.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | And 3 more authors.
Environmental Toxicology and Pharmacology | Year: 2016

The aim of the study was to investigate the influence of subacute exposure to lead on the glutathione-related antioxidant defense and oxidative stress parameters in 36 males occupationally exposed to lead for 40 ± 3.2 days.Blood lead level in the examined population increased significantly by 359% due to lead exposure. Simultaneously, erythrocyte glutathione level decreased by 16%, whereas the activity of glutathione-6-phosphate dehydrogenase in erythrocytes and leukocytes decreased by 28% and 10%, respectively. Similarly, the activity of glutathione-S-transferase in erythrocytes decreased by 45%. However, the activity of glutathione reductase in erythrocytes and leukocytes increased by 26% and 6%, respectively, whereas the total oxidant status value in leukocytes increased by 37%.Subacute exposure to lead results in glutathione pool depletion and accumulation of lipid peroxidation products; however, it does not cause DNA damage. Besides, subacute exposure to lead modifies the activity of glutathione-related enzymes. © 2016 Elsevier B.V. Source


Gac P.,Wroclaw Medical University | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Poreba R.,Wroclaw Medical University | Poreba M.,Wroclaw Medical University | And 3 more authors.
Environmental Toxicology and Pharmacology | Year: 2012

Purpose: Present study aimed at determining blood selenium concentration (Se-B) in a selected population of children inhabiting industrial regions. Methods: The studieswere conducted on a group of 267 children inhabiting industrial regions in Upper Silesia (Poland). Determination of Se-B was performed using hydride generation atomic absorption technique. Results: In the studied group of children mean Se-B amounted to 76.75±12.52 μg/L. Only in 38.20% of studied children the estimated Se-B could be regarded as an optimal. Children with underweight (BMI < 15th centile) manifested a significantly lower Se-B than children with BMI within the normal range (BMI 15-85th centile). In the entire study group of children a significant positive linear correlation was disclosed between BMI and Se-B (r = 0.16, p < 0.05). Regression analysis demonstrated that in the studied subgroup of children (with normal height, body mass and BMI) a higher BMI represented an independent factor of Se-B increase and a more pronounced age an independent factor of Se-B decrease. Moreover, maternal education represented an independent factor of Se-B increase in the studied group of children (with normal height, body mass and BMI). Conclusions: Se-B in the studied group children from industrial area in Upper Silesia corresponded with the lower range of blood selenium concentrations noted in recent years in other populations. A negative relationship was documented between age and Se-B and a positive relationship between BMI of a child and maternal education on one hand and Se-B of a child on the other. © 2012 Elsevier B.V. Source


Gac P.,Wroclaw Medical University | Pawlas N.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Wylezek P.,Institute of Occupational Medicine and Environmental Health in Sosnowiec | Poreba R.,Wroclaw Medical University | And 3 more authors.
Biological Trace Element Research | Year: 2016

This study aimed at evaluation of a relationship between blood selenium concentration (Se-B) and blood cystatin C concentration (CST) in a randomly selected population of healthy children, environmentally exposed to lead and cadmium. The studies were conducted on 172 randomly selected children (7.98 ± 0.97 years). Among participants, the subgroups were distinguished, manifesting marginally low blood selenium concentration (Se-B 40–59 μg/l), suboptimal blood selenium concentration (Se-B: 60–79 μg/l) or optimal blood selenium concentration (Se-B ≥ 80 μg/l). At the subsequent stage, analogous subgroups of participants were selected separately in groups of children with BMI below median value (BMI <16.48 kg/m2) and in children with BMI ≥ median value (BMI ≥16.48 kg/m2). In all participants, values of Se-B and CST were estimated. In the entire group of examined children no significant differences in mean CST values were detected between groups distinguished on the base of normative Se-B values. Among children with BMI below 16.48 kg/m2, children with marginally low Se-B manifested significantly higher mean CST values, as compared to children with optimum Se-B (0.95 ± 0.07 vs. 0.82 ± 0.15 mg/l, p < 0.05). In summary, in a randomly selected population of healthy children no relationships could be detected between blood selenium concentration and blood cystatin C concentration. On the other hand, in children with low body mass index, a negative non-linear relationship was present between blood selenium concentration and blood cystatin C concentration. © 2016 Springer Science+Business Media New York Source

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