Czech B.,University of Silesia |
Lodowski P.,University of Silesia |
Marczak W.,Institute of Occupational Medicine and Environmental Health
Chemical Physics Letters | Year: 2013
Experimental excess molar isentropic compressions for six binary systems: (pyridine, 2-methylpyridine or 2,6-dimethylpyridine) + (water or methanol) are correlated with the calculated theoretically association energies of the ROH⋯NA complexes that increase in the order: pyridine < 2-methylpyridine < 2,6-dimethylpyridine. In spite of stronger O-H⋯N bonds, the negative excesses for methanolic systems are smaller than for the aqueous ones. That is mainly because of the aggregation of water-amine complexes due to the O-H⋯O bonds. Differences in partial molar compressions of the amines in water and methanol solvents result most probably from the hydrophobic hydration in aqueous solutions. © 2012 Elsevier B.V. All rights reserved.
Sas-Nowosielska H.,Nencki Institute of Experimental Biology |
Pawlas N.,Institute of Occupational Medicine and Environmental Health
Acta Biochimica Polonica | Year: 2015
People are exposed to heavy metals both in an occupational and natural environment. The most pronounced effects of heavy metals result from their interaction with cellular genetic material packed in form of chromatin. Heavy metals influence chromatin, mimicking and substituting natural microelements in various processes taking place in the cell, or interacting chemically with nuclear components: nucleic acids, proteins and lipids. This paper is a review of current knowledge on the effects of heavy metals on chromatin, exerted at the level of various nuclear components.
Brewczynski P.Z.,Children of the Institute of Occupational Medicine and Environmental Health |
Brodziak A.,Institute of Occupational Medicine and Environmental Health
Medical Science Monitor | Year: 2015
Studies show that a significant proportion of young people suffering from childhood onset asthma later recovered, usually in adolescence. In this article we argue that an understanding of the differences between children who recover from asthma and those who do not would contribute to increased understanding of the pathogenic mechanisms of the disease and could provide new clues about prevention and treatment. We note that some researchers have recently published results from these kinds of investigations. This paper reports results regarding genetic determinants, distorted mechanisms of inflammation, and mind/body relationships. We also try to integrate findings from these 3 areas to formulate general conclusions about the pathogenesis of asthma. © Med Sci Monit.
Pavanello S.,University of Padua |
Dioni L.,University of Milan |
Hoxha M.,University of Milan |
Fedeli U.,Sistema Epidemiologico Regione Veneto SER |
And 2 more authors.
Cancer Epidemiology Biomarkers and Prevention | Year: 2013
Background: Increased mitochondrial DNA copy number (mtDNAcn) is a biologic response to mtDNA damage and dysfunction, predictive of lung cancer risk. Polycyclic aromatic hydrocarbons (PAHs) are established lung carcinogens and may cause mitochondrial toxicity. Whether PAH exposure and PAH-related nuclear DNA (nDNA) genotoxic effects are linked with increased mtDNAcn has never been evaluated. Methods: We investigated the effect of chronic exposure to PAHs on mtDNAcn in peripheral blood lymphocytes (PBLs) of 46 Polish male noncurrent smoking coke-oven workers and 44 matched controls, who were part of a group of 94 study individuals examined in our previous work. Subjects' PAH exposure and genetic alterations were characterized through measures of internal dose (urinary 1-pyrenol), target dose [antibenzo[ a]pyrene diolepoxide (anti-BPDE)-DNA adduct], genetic instability (micronuclei and telomere length), and DNA methylation (p53 promoter) in PBLs. mtDNAcn (MT/S) was measured using a validated real-time PCR method. Results: Workers with PAH exposure above the median value (>3 mmol 1-pyrenol/mol creatinine) showed highermtDNAcn[geometric means (GM) of 1.06 (unadjusted) and 1.07 (age-adjusted)] compared with controls [GM 0.89 (unadjusted); 0.89 (age-adjusted); (P = 0.029 and 0.016)], as well as higher levels of genetic and chromosomal [i.e., anti-BPDE-DNA adducts (P < 0.001), micronuclei (P < 0.001), and telomere length (P=0.053)] and epigenetic [i.e., p53 gene-specific promoter methylation (P < 0.001)] alterations in the nDNA. In the whole study population, unadjusted and age-adjusted mtDNAcn was positively correlated with 1-pyrenol (P = 0.043 and 0.032) and anti-BPDE-DNA adducts (P = 0.046 and 0.049). Conclusions:PAHexposure and PAH-relatednDNAgenotoxicity are associated with increased mtDNAcn. Impact: The present study is suggestive of potential roles of mtDNAcn in PAH-induced carcinogenesis. © 2013 American Association for Cancer Research.
Lis D.O.,Institute of Occupational Medicine and Environmental Health |
Gorny R.L.,Central Institute for Labour Protection
American Journal of Infection Control | Year: 2013
Background: The aim of this study was to assess the exposure of children to airborne Haemophilus influenzae in day care centers. Methods: Air samples were taken using an Andersen impactor in 32 rooms designed for children stay. The concentrations of airborne bacteria were calculated as colony forming units (CFU) (growing on trypticase soy agar) per cubic meter of air (CFU/m 3). The compositions of bioaerosol were determined on blood trypticase soy agar and Haemophilus selective agar. Isolated strains were identified using API NH strips and apiweb software. The antibiotic resistance of H influenzae strains was determined by the disk diffusion method. Results: Compared with the proposed criteria for microbiologic quality of indoor air, the rooms were characterized by the very high bacterial contamination of the air. The prevailing component of bacterial aerosol was gram-positive cocci. Airborne H influenzae strains were found in 25% of the investigated rooms and were mostly classified as biotype II (33%). Conclusion: It may be accepted that the exposure to airborne H influenzae is typical of child day care centers in contrast to indoor environments with older population. Child day care center contribute to the expansion of H influenzae in human population via air. Generally, airborne H influenzae isolates from the investigated child day care centers were susceptible to older antibiotics such as ampicillin and amoxicillin-clavulanic acid. Copyright © 2013 by the Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.