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Ren R.,Harbin Medical University | Zhang Y.,Harbin Medical University | Lee B.,Institute of Occupational Disease Prevention | Wu Y.,Harbin Medical University | Li B.,Harbin Medical University
Journal of Cellular Biochemistry | Year: 2011

The aim of this study was to assess the effect of the β-amyloid fragment Aβ25-35 on mitochondrial structure and function and on the expression of proteins associated with the mitochondrial permeability transition pore (MPTP) in rat hippocampal neurons. Ninety clean-grade Sprague-Dawley rats were randomly assigned to six groups (n = 15 per group). Aβ25-35 (1, 5, or 10 Âμg/rat) was injected into hippocampal area CA1. Normal saline was injected as a control. The effect of Aβ25-35 injection on hippocampal structure was assessed by transmission electron microscopy. Ca2+-ATPase activity, [Ca 2+]i, and mitochondrial membrane potential were measured. The expression of genes associated with the MPTP, including the voltage-dependent anion channel (VDAC), adenine nucleotide translocator (ANT), and cyclophilin D (Cyp-D), were evaluated. Results showed that Aβ25-35 injection damaged the mitochondrial structure of hippocampal neurons, decreased Ca2+-ATPase activity and mitochondrial membrane potential, and increased [Ca2+]i. The expression levels for VDAC, ANT, and Cyp-D in all groups were significantly (P < 0.05) higher than those in the normal control group after Aβ25-35 injection. These results indicate that Aβ25-35 damages mitochondria in rat hippocampal neurons and effects mitochondrial dysfunction, as well as increasing the expression of genes associated with the MPTP. Mitochondrial dysfunction may result in increased MPTP gene expression, leading to neurodegenerative effects. © 2011 Wiley-Liss, Inc. Source


Liu Z.-Y.,Jiangxi Agricultural University | Liu Z.-Y.,Institute of Occupational Disease Prevention | Wang Z.-L.,Jiangxi Agricultural University | Wu X.-B.,Jiangxi Agricultural University | And 2 more authors.
Insect Science | Year: 2011

The single locus complementary sex determination (sl-csd) gene is the primary gene determining the gender of honey bees (Apis spp.). While the csd gene has been well studied in the Western honey bee (Apis mellifera), and comparable data exist in both the Eastern honey bee (Apis cerana) and the giant honey bee (Apis dorsata), no studies have been conducted in the red dwarf honey bee, Apis florea. In this study we cloned the genomic region 3 of the A. florea csd gene from 60 workers, and identified 12 csd alleles. Analysis showed that similar to A. mellifera, region 3 of the csd gene contains a RS domain at the N terminal, a proline-rich domain at the C terminal, and a hypervariable region in the middle. However, the A. florea csd gene possessed a much higher level of nucleotide diversity, compared to A. mellifera, A. cerana and Apis dorsata. We also show that similar to the other three Apis species, in A. florea, nonsynonymous mutations in the csd gene are selectively favored in young alleles. © 2011 The Authors Journal compilation © Institute of Zoology, Chinese Academy of Sciences. Source


Wu B.,Nanjing Medical University | Ji X.,Nanjing Medical University | Han R.,Nanjing Medical University | Han L.,Nanjing Medical University | And 4 more authors.
Immunology Letters | Year: 2014

Background: Glucocorticoid-induced tumor necrosis factor (TNF) receptor-related protein ( GITR) mainly affects the functions of effector T cells and regulatory T cells thus it may influence various diseases. Coal workers' pneumoconiosis (CWP) is a serious occupational disease worldwide. In the present study, we examined the association between the functional polymorphisms in GITR and risk of CWP in a Chinese population. Methods: An association study analyzing three polymorphisms (rs3753348, rs2298213, and rs11466668) in GITR were performed in a case-control study including 693 patients with CWP and 690 controls. Genotyping was carried out by Taqman method. Results: The GITR rs3753348 GG/GC genotypes significantly enhanced the risk of CWP (adjusted OR. = 1.32, 95%CI. = 1.02-1.71), compared with the CC genotype, particularly among subgroups of long exposure years (adjusted OR. = 1.47, 95%CI. = 1.06-2.04) and non-smokers (adjusted OR. = 1.45, 95%CI. = 1.01-2.09). Moreover, the polymorphism was significantly associated with risk for CWP cases with stage II. Conclusions: This is the first report revealing an association between the GITR rs3753348 polymorphism and CWP, and our results suggest that the GITR rs3753348 polymorphism may be involved in the development and susceptibility of CWP. © 2014 Elsevier B.V. Source


Wang Z.,Jiangxi Agricultural University | Liu Z.,Institute of Occupational Disease Prevention | Wu X.,Jiangxi Agricultural University | Yan W.,Jiangxi Agricultural University | Zeng Z.,Jiangxi Agricultural University
Molecular Biology Reports | Year: 2012

The complementary sex determination (csd) gene is the primary gene determining the gender of honey bees (Apis spp). In this study we analyzed the polymorphism of csd gene in six Apis mellifera subspecies. The genomic region 3 of csd gene in these six A. mellifera was cloned, and identified. A total of 79 haplotypes were obtained from these six subspecies. Analysis showed that region 3 of csd gene has a high level of polymorphism in all the six A. mellifera subspecies. The A. m. anatolica subspecies has a slightly higher nucleotide diversity (π) than other subspecies, while the π values showed no significant difference among the other five subspecies. The phylogenetic tree showed that all the csd haplotypes from different A. mellifera subspecies are scattered throughout the tree, without forming six different clades. Population differentiation analysis showed that there are significant genetic differentiations among some of the subspecies. The NJ phylogenetic tree showed that the A. m. caucasica and A. m. carnica have the closest relationship, followed by A. m. ssp, A. m. ligustica, A. m. carpatica and A. m. anatolica that were gathered in the tree in turn. © Springer Science+Business Media B.V. 2011. Source


Shen H.,Modern Medicine | Shen H.,Institute of Occupational Disease Prevention | Huo X.,Modern Medicine | Liu K.,Nanjing Medical University | And 11 more authors.
Journal of Occupational and Environmental Medicine | Year: 2012

OBJECTIVES:: To investigate whether glutathione S-transferases (GST) genetic polymorphisms (GSTT1 rs1049055, GSTM1 rs10712361, and GSTP1 rs1695) are associated with susceptibility to noise-induced hearing loss (NIHL). METHODS:: These polymorphisms were analyzed in 444 NIHL and 445 normal hearing workers. In addition, total plasma GST activity was measured in all subjects. RESULTS:: Individuals with the GSTM1 null genotype had a statistically significantly increased risk of NIHL (odds ratio [OR] = 1.64, 95% confidence interval [CI] = 1.26 to 2.13) compared with those carrying a wild-type GSTM1 genotype. This effect was more pronounced among the workers exposed to 86 to 91 dB(A) (OR = 3.35, 95% CI = 1.54 to 7.31). Glutathione S-transferase activity of the NIHL workers was also lower than that of normal hearing workers (14.5 ± 5.1 U/ml vs 15.9 ± 6.3 U/ml, P = 0.010). CONCLUSION:: Our results suggest that GSTM1 polymorphism is associated with susceptibility to NIHL. Copyright © 2012 by American College of Occupational and Environmental Medicine. Source

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