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Wang H.J.,Institute of Nutrition and Food Hygiene
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2011

To analyze the status and trend of dietary fiber intake and its food sources among Chinese residents aged 18 - 45 years old in 9 provinces (autonomous region), from 1989 to 2006. Twenty-four-hour dietary recall data of three consecutive days from China Health and Nutrition Survey (1989, 1991, 1993, 1997, 2000, 2004 and 2006) were used. And the 18 - 45 years old subjects with complete information on dietary survey were included. The sample size of these 7 surveys were 5597, 5596, 5323, 5485, 5308, 4144 and 3889, respectively. The trends of dietary fiber intake and food sources by time and sex were studied. From 1989 to 2000, the total of daily dietary fiber intake of 18 - 45 years old healthy residents decreased from 15.1 g/d to 11.6 g/d, insoluble dietary fiber intake decreased from 22.6 g/d to 17.8 g/d. In villages, from 1989 to 2000, the insoluble dietary fiber intake decreased 5.1 g/d in males and 5.2 g/d in females(13.2, 11.8 g in 2000, respectively;18.3, 17.0 g in 1989, respectively). In 2006, the insoluble dietary intake was 12.6 g/d in males and 11.2 g/d in females in villages. In cities, from 1989 to 2006, the insoluble dietary fiber intake was 9.4 - 11.8 g/d in males and 8.3 - 10.8 g/d in females. The total dietary fiber intake was 15.7 - 17.6 g/d in males and 13.5 - 16.4 g/d in females. The difference of insoluble and total dietary fiber intake between city and village was decreasing. In 2006, 70.6% (8.4/11.9) insoluble dietary fiber came from vegetables ((4.1 ± 3.1) g/d), wheat flour and its products ((2.6 ± 3.6) g/d), rice and its products ((1.7 ± 1.3) g/d). Dietary fiber intake among Chinese aged 18 - 45 in 9 provinces (autonomous region) decreased from 1989 to 2006. Vegetables, wheat flour, rice and their products were the major food sources of insoluble dietary fiber. Source

Sun Z.,Capital Medical University | Guan X.,Capital Medical University | Li N.,Institute of Nutrition and Food Hygiene | Liu X.,Capital Medical University | And 2 more authors.
Oral Oncology | Year: 2010

ZengShengPing (ZSP), a mixture of six medicinal herbs, has been reported to prevent esophageal squamous cell carcinoma (SCC) in human patients with dysplasia. This study was designed to investigate the chemopreventive effects of ZSP on oral cancer in animal models and human patients. In the 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster cheek pouch model, ZSP (6 g/kg BW/day by gavage for 10 weeks) significantly reduced the number of visible tumor, the tumor volume, and the incidence of SCC (P < 0.01). Two biomarkers associated with cell proliferation, silver stained nucleolar organizer region (AgNOR) and proliferating cell nuclear antigen (PCNA)-labeling index, were also significantly suppressed by ZSP treatment (P < 0.01). In the 4-nitroquinoline 1-oxide (4NQO)-induced oro-esophageal cancer model in mice, ZSP (10% in diet) also significantly reduced the incidence of tongue SCC from 55.2% (16/29) to 22.2% (6/27) (P < 0.05), and slightly reduced the incidence of esophageal SCC from 34.5% (10/29) to 22.2% (6/27). Furthermore, in a randomized clinical trial on patients with oral leukoplakia, ZSP (4 tablets, 3 times per day for 8-12 months) reduced the size of oral lesion in 67.8% (40/59) patients, whereas the placebo was effective in 17% (9/53) patients (P < 0.01). Such an effect was associated with significant decrease of AgNOR and PCNA-labeling index. In summary, our studies have demonstrated the chemopreventive effects of ZSP on two animal models of oral cancer, and human patients with oral leukoplakia. © 2009. Source

Hou X.,China Agricultural University | Guo K.,China Agricultural University | Bao J.,Beijing Animal Health Inspection Institute | Wu Y.,Institute of Nutrition and Food Hygiene | Li H.,China Agricultural University
Journal of Food, Agriculture and Environment | Year: 2011

Ofloxacin polyclonal antibody was prepared and cross-reactivity to other fluroquinones was observed. Further, a rapid, low-cost ELISA analytical technique was developed, with IC 50 at 1.2 ng.mL -1, for the analysis of ofloxacin in the environment matrix including wastewater, manure and sludge. The mean recoveries and coefficients of variation were in a range of 74-83% and 8.5-12.2%, respectively, in wastewater. The mean recoveries and coefficients of variation in pig manure and sludge were from 62% to 78% and from 8.4% to 14.4%, respectively. Concentrations of ofloxacin using ELISA were sometimes greater than measured with LC-MS/MS. It may be due to cross-reactivity to structurally similar compounds or metabolites. Results from this study showed that the ELISA can be used as a preliminary screening tool for later instrumental analysis. Source

Hou X.,China Agricultural University | Li Y.,Circulation Industry Promotion Center | Zhang Z.,China Agricultural University | Wu Y.,Institute of Nutrition and Food Hygiene
Chromatographia | Year: 2010

Matrix solid-phase dispersion (MSPD) with alumina N as adsorbent has been used for extraction of para red, Sudan 1, Sudan 2, Sudan 3, and Sudan 4 dyes from egg yolk. The extracts were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS). Mean recovery for the five dyes ranged from 63.2 to 98.6%, with CV 0.55-10.00%. One sample was confirmed to contain 0.3 mg kg-1 Sudan 4. © 2009 Vieweg+Teubner | GWV Fachverlage GmbH. Source

Sai F.,Institute of Nutrition and Food Hygiene | Sai F.,Chinese Center for Food Safety Risk Assessment | Hong M.,Chinese Center for Food Safety Risk Assessment | Hong M.,U.S. Center for Disease Control and Prevention | And 6 more authors.
Journal of Agricultural and Food Chemistry | Year: 2012

A sensitive method has been developed for the simultaneous determination of residues of 25 β2-agonists and 23 β-blockers in animal foods by high-performance liquid chromatography coupled with linear ion trap mass spectrometry (HPLCLIT-MS). This method is based on a new procedure of hydrolysis and extraction by 5% trichloracetic acid, and then cleaned up by mixed strong cation exchange (MCX) cartridges coupled with a novelty cleanup step by methanol. Methanol and 0.1% formic acid were used as mobile phases for gradient elution, while a Supelco Ascentis Express Rp-Amide column was used for LC separation. ESI positive ion scan mode was used with consecutive reaction monitoring (CRM, MS 3). Nine β 2-agonists labeled by the deuterium isotope were used as internal standards for quantification. The linear ranges of 48 analytes were from 5 to 200 μg/L; the coefficient of correlation was not less than 0.995. Blank pork muscle, blank liver, and blank kidney were selected as representative matrix for spiked standard recovery test. The recoveries of each compound were in the range of 46.6-118.9%, and the relative standard deviations were in the range of 1.9-28.2%. Decision limits (CCα, α = 0.01) of 48 analytes in muscles, liver, and kidney samples ranged from 0.05 to 0.49 μg/kg, and the detection capability (CCβ, β = 0.05) ranged from 0.13 to 1.64 μg/kg. This method was successfully applied to 110 real animal origin food samples including meat, liver, and kidney of pig and chicken samples. © 2012 American Chemical Society. Source

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