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Takala P.N.,INRS Institute Armand Frappier | Salmieri S.,INRS Institute Armand Frappier | Salmieri S.,Institute of Nutraceuticals and Functional Foods INAF | Boumail A.,INRS Institute Armand Frappier | And 6 more authors.
Journal of Food Engineering | Year: 2013

Bioactive trilayer films were prepared using methylcellulose (MC) and polycaprolactone (PCL). Two antimicrobial formulations named as A (organic acids, extract of rosmarinic acid and Asian essential oil (EO) mixture) and B (organic acids, extract of rosmarinic acid and Italian EO mixture) were added in MC films during casting and the trilayer composite films (PCL/MC/PCL) were fabricated using compression molding. These films were inserted into packages containing broccoli and the packages were kept at 4 °C during 12 days storage to determine the antimicrobial capacity of the films and the physico-chemical properties of the films. Bioactive films showed a significant reduction of Escherichia coli in broccoli from Day 4 and a total inhibition at Day 12. Similarly, these films showed a significant reduction of Salmonella typhimurium from Day 2 and a total inhibition at Day 7. Moreover, the films controlled the growth of total aerobic microbiota (TAM) in broccoli up to 10 days. Encapsulation of antimicrobial compounds in films allowed decrease of barrier properties of films. Tensile strength of films was not affected by the presence of formulation B. These results demonstrated the potential application of trilayer antimicrobial on controlling food pathogens and total flora in pre-cut vegetables. © 2013 Elsevier Ltd. All rights reserved. Source

Bazinet L.,Institute of Nutraceuticals and Functional Foods INAF | Schreyer A.,Institute of Nutraceuticals and Functional Foods INAF | Lessard J.,Universite de Sherbrooke
Dairy Science and Technology | Year: 2011

Milk degradation during processing and storage is mainly due to oxidation-reduction reactions. Electroreduction was proposed recently to modulate the redox potential of food such as milk. However, only one study was carried-out on milk with a hydrodynamic electroreduction cell, and this study concluded, according to its protocol, that 4 V was the best anode/cathode voltage difference. But, since electroreduction is an electromembrane process, energy consumption is a major concern. Optimization of milk electroreduction can be one of the ways for improving milk resistance to alteration. Our objectives in the present work were to (1) investigate the effect of low anode/cathode voltage differences on milk redox potential modulation during electroreduction, (2) optimize the process, and (3) compare storage of a low-voltage electroreduced milk with a non-electroreduced milk. It appeared from these results that electroreduction at anode/cathode voltage difference of 3 V was sufficient to ensure a significant decrease in redox potential and dissolved oxygen and allow an energy saving of 79% in comparison with a treatment at 4 V. It appeared also that oxygen is an important parameter to consider during storage of electroreduced milk. To our knowledge, this study is the first one to demonstrate that electroreduction process can be a technological and chemical free alternative to improve stability against oxidation with a low-energy consumption. © INRA and Springer Science+Business Media B.V. 2011. Source

Couet N.,Laval University | Desroches S.,Laval University | Desroches S.,Institute of Nutraceuticals and Functional Foods INAF | Robitaille H.,University of Quebec | And 6 more authors.
Health Expectations | Year: 2015

Background: We have no clear overview of the extent to which health-care providers involve patients in the decision-making process during consultations. The Observing Patient Involvement in Decision Making instrument (OPTION) was designed to assess this. Objective: To systematically review studies that used the OPTION instrument to observe the extent to which health-care providers involve patients in decision making across a range of clinical contexts, including different health professions and lengths of consultation. Search strategy: We conducted online literature searches in multiple databases (2001-12) and gathered further data through networking. Inclusion criteria: (i) OPTION scores as reported outcomes and (ii) health-care providers and patients as study participants. For analysis, we only included studies using the revised scale. Data extraction: Extracted data included: (i) study and participant characteristics and (ii) OPTION outcomes (scores, statistical associations and reported psychometric results). We also assessed the quality of OPTION outcomes reporting. Main results: We found 33 eligible studies, 29 of which used the revised scale. Overall, we found low levels of patient-involving behaviours: in cases where no intervention was used to implement shared decision making (SDM), the mean OPTION score was 23 ± 14 (0-100 scale). When assessed, the variables most consistently associated with higher OPTION scores were interventions to implement SDM (n = 8/9) and duration of consultations (n = 8/15). Conclusions: Whatever the clinical context, few health-care providers consistently attempt to facilitate patient involvement, and even fewer adjust care to patient preferences. However, both SDM interventions and longer consultations could improve this. © 2013 John Wiley & Sons Ltd. Source

Ndiaye N.,Institute of Nutraceuticals and Functional Foods INAF | Ndiaye N.,Laval University | Pouliot Y.,Institute of Nutraceuticals and Functional Foods INAF | Pouliot Y.,Laval University | And 6 more authors.
Separation and Purification Technology | Year: 2010

Bovine lactoferrin (LF), a 80 kDa iron-binding glycoprotein, has been reported to have important nutraceutical and biological properties such as anti-inflammatory, antimicrobial and immunostimulatory activities. However, the large scale utilisation of LF requires a cost-effective purification process. The aim of this study was to evaluate the feasibility of separating lactoferrin from whey using electrodialysis with an ultrafiltration membrane (EDUF) system and to study the effect of pH on this protein migration rate during EDUF treatments. Initially, to set the optimum conditions for electroseparation of this molecule, its electrophoretic mobility was measured according to the pH (pH 3-12). LF had an optimal electrophoretic mobility at pH 3.0 of 1.5×10 -8m 2 V -1 s -1 in a 2 g/L KCl solution and of 3.0×10 -8m2 V -1 s -1 in distilled water. Thereafter, a 0.1% LF solution at pH 3.0 was treated by EDUF with an ultrafiltration membrane of 500 kDa molecular weight cut-off. A migration rate going up to 46% was obtained after 4 h of treatment. Finally, the EDUF process was applied on lactoferrin-enriched-whey solutions at pH values of 3.0, 4.0, and 5.0. The highest migration rate for lactoferrin was obtained at pH 3.0 with a migration yield of 15%. Thus, it appeared that the EDUF process could allow the separation of large proteins, such as LF, from a solution. Furthermore, the selectivity of EDUF was decreased in whey solution due to concomitant migration of β-lactoglobulin or other whey proteins. However, the fraction obtained at pH 3.0 presented β-lactoglobulin percentages close to the ones obtained for WPI by ion-exchange technology. © 2010 Elsevier B.V. All rights reserved. Source

Dolley G.,Laval University | Dolley G.,Institute of Nutraceuticals and Functional Foods INAF | Lamarche B.,Laval University | Lamarche B.,Institute of Nutraceuticals and Functional Foods INAF | And 6 more authors.
Molecular Genetics and Metabolism | Year: 2011

The small, dense LDL phenotype is associated with an increased cardiovascular disease risk. A genome-wide scan performed on 236 nuclear families of the Quebec Family Study (QFS) revealed a quantitative trait locus affecting LDL peak particle diameter (LDL-PPD) and density on the 1p31 region. This region contains the low-density lipoprotein receptor-related protein 8 (LRP8) gene. LRP8, a receptor for apolipoprotein (apo) E, modulates apoE levels, thus contributing to plasma cholesterol and triglyceride (TG) concentrations. We investigate the effects of LRP8 polymorphisms on LDL-PPD, on the relative proportion of small LDL (< 255. Å) and the absolute concentration of cholesterol among the small LDL particles. LRP8 rs5174 was associated with LDL-PPD and estimated cholesterol concentrations in the small LDL particles adjusted for the effects of age and sex (p = 0.008, p = 0.04, respectively). LRP8 rs3820198 was associated with total and LDL-cholesterol levels as well as with apoB concentrations adjusted for the effects of age and sex (p = 0.005, p = 0.004 and p = 0.01, respectively) but not with LDL size-related variables. These results suggest that LRP8 gene polymorphisms influence plasma cholesterol levels as well as size and composition of LDL particles. © 2010 Elsevier Inc. Source

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