Institute of Natural Fibers and Medicinal Plants

Poznań, Poland

Institute of Natural Fibers and Medicinal Plants

Poznań, Poland
Time filter
Source Type

Seremak-Mrozikiewicz A.,Poznan University of Medical Sciences | Drews K.,Poznan University of Medical Sciences | Wender-Ozegowska E.,Poznan University of Medical Sciences | Mrozikiewicz P.M.,Poznan University of Medical Sciences | Mrozikiewicz P.M.,Institute of Natural Fibers and Medicinal Plants
Journal of Thrombosis and Thrombolysis | Year: 2010

Many studies established that gestational hypertension (GH) and preeclampsia (PE) are multifactorial diseases and disturbances in coagulation cascade have etiological significance. Inherited thrombophilias, like polymorphism of factor V (FV) Leiden and prothrombin (PTM) are considered to be involved in the PE development. The aim of this study was to determine the association between FV Leiden and G20210A of PTM gene polymorphism and GH/PE appearance. The study comprised 235 women: GH (n = 126, mean age 27.5 ± 6.0 years), mild PE (n = 41, mean age 28.3 ± 5.7 years), and severe PE (n = 68, mean age 28.5 ± 5.7 years). The control group consisted of 400 healthy pregnant women (mean age 27.5 ± 4.7 years). All women included in the study were white Caucasian of Polish origin, and were singleton pregnancies. The G1691A polymorphism of FV and G20210A polymorphism of PTM were detected using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) assays. For PTM G20210A polymorphism overrepresentation of heterozygous GA genotype (7.4 vs. 1.2%, P = 0.02) and of A allele (3.7 vs. 0.6%, P = 0.02) in the group of severe PE have been found. For FV G1691A polymorphism the overrepresentation of genotypes containing at least one mutated allele A (GA and AA) in the group of women with mild (9.7 vs. 3.5%, ns) and severe PE (8.8 vs. 3.5%, ns) was observed. Our results suggest the significant influence of G20210A prothrombin polymorphism and possible influence of G1691A factor V polymorphism in the development of severe preeclampsia. © 2009 Springer Science+Business Media, LLC.

Seremak-Mrozikiewicz A.,Poznan University of Medical Sciences | Drews K.,Poznan University of Medical Sciences | Barlik M.,Poznan University of Medical Sciences | Sieroszewski P.,Medical University of Lódz | And 3 more authors.
Journal of Maternal-Fetal and Neonatal Medicine | Year: 2011

Objective. Preeclampsia (PE) is believed to be induced by endothelial cell dysfunction in placenta. Highly polymorphic endothelial nitric oxide synthase (eNOS) activity belongs to the factors significantly influencing vaso-motor tone in placenta and PE susceptibility. The aim of this study was to evaluate prevalence of -786T/C polymorphism of eNOS gene in the groups of women with mild and severe PE. Study design. The study was performed in the group of 218 preeclamptic (including 136 with severe PE) and of 400 normotensive healthy women delivered normally after a healthy gestation. The eNOS -786T/C polymorphism was determined using PCR/RFLP assay. Additionally, detailed correlation between eNOS genotypes and clinical/laboratory data in the PE group has been analyzed. Results. The higher frequency of mutated homozygous CC genotypes (17.4% vs. 11.5% in controls, OR 1.62, n.s.) and of C alleles (allelic frequency 44.1 vs. 36.6%; OR 1.36, p = 0.012) in the group of PE has been determined. Furthermore, in the group of severe PE the overrepresentation of mutated CC genotypes (23.5% vs. 11.5%, OR 2.37, p = 0.0014) and mutated C alleles (47.8 vs. 36.6%, OR 1.58, p = 0.0016) has been found. Conclusions. The presence of mutated homozygous CC genotype and C allele of -786T/C polymorphism of eNOS gene influences the higher susceptibility to develop severe PE development. © 2011 Informa UK, Ltd.

PubMed | Institute of Natural Fibers and Medicinal Plants and Poznan University of Medical Sciences
Type: Journal Article | Journal: Molecular medicine reports | Year: 2016

Polycystic ovary syndrome (PCOS) is a common endocrine disorder in women of reproductive age. A number of PCOS complications may be associated with the elevated level of angiotensinII and low bradykinin concentrations. The aim of the present study was to investigate the frequencies of angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) polymorphisms in women with PCOS and to determine the association between ACE genetic variants and the risk of metabolic and cardiovascular complications in such women. A total of 138PCOS patients and 110healthy volunteers were enrolled. Cardiovascular risk was estimated according to the criteria of the American Heart Association and Androgen Excess and PCOS Society. The median average age was 28.5 (26.031.0) and 27.0 (24.030.0) years in the control and PCOS groups, respectively (P=0.004). Anthropometric parameters, including body mass index and waist circumference were significantly higher in the PCOS patients. In the PCOS group, 97 (57.4%) of the subjects were metabolically unhealthy, whereas, in the control group 51 (46.4%) subjects were (P=0.07). The II, ID, and DD genotypes frequencies were 29.1, 44.5, and 26.4% in the controls and 5.0, 37.7, and 57.3% in the PCOS patients. The cardioprotective Iallele was observed significantly less frequently in the women with PCOS compared with the controls [odds ratio (OR), 3.27; P=0.0001]. The DDgenotype, which is known to increase cardiovascular risk, was more frequently observed in PCOS patients (OR, 3.87; P=0.0003), whereas the cardioprotective IIgenotype occurred in this group less frequently (OR, 0.4; P=0.06). The results of the present study demonstrated a statistically significant association between the ACE I/D polymorphism and the presence and intensity of metabolic disturbances in women with PCOS.

PubMed | Institute of Natural Fibers and Medicinal Plants, Poznan University of Medical Sciences and University of Life Sciences in Poznań
Type: Journal Article | Journal: Central-European journal of immunology | Year: 2016

The aim of the study was to evaluate analgesic activity (hot plate test), anti-inflammatory activity (carrageenan-induced paw edema) and locomotor activity in rats under the influence of three fractions of Chelidonium majus herb extract: full water extract (FWE), protein enriched fraction (PEF), and non-protein fraction (NPF). Effects of the fractions on the level of chosen cytokines and their mRNA levels were also assessed using lipopolysaccharide (LPS) administration as a proinflammatory cue. All fractions and diclofenac did not affect the locomotor activity of rats in comparison with the control group. FWE and PEF three hours after administration showed statistically significant analgesic activities comparable to morphine (p < 0.05). A slight reduction in rat paw edema was observed after three (comparable with diclofenac) and six hours in the NPF group. FWE revealed a statistically significant pro-inflammatory effect after three hours in comparison with the control group. Peripheral IL-1 and IL-4 cytokine concentrations were reduced under FWE and NPF, PEF fractions. The combination of FWE, PEF and NPF together with LPS showed only the effects of LPS. We suggest that protein enriched fraction (PEF) produced centrally mediated (morphine-like) analgesic action, whereas the anti-inflammatory potential was shown only after LPS-induced inflammation. The precise mechanisms involved in the production of anti-nociceptive and anti-inflammatory responses of studied fractions are not completely understood, but they may be caused rather by the presence of protein more than alkaloids-enriched fraction. This fraction of the extract could be used as an alternative therapy for the prevention of inflammatory-related diseases in the future, but further studies are needed.

Pietrzyk A.J.,Polish Academy of Sciences | Bujacz A.,Technical University of Lodz | Mueller-Dieckmann J.,University of Hamburg | Lochynska M.,Institute of Natural Fibers and Medicinal Plants | And 4 more authors.
Acta Crystallographica Section D: Biological Crystallography | Year: 2013

The first crystal structure of a complex formed by two storage proteins, SP2 and SP3, isolated from their natural source, mulberry silkworm (Bombyx mori L.) haemolymph, has been determined. The structure was solved by molecular replacement using arylphorin, a protein rich in aromatic amino-acid residues, from oak silkworm as the initial model. The quality of the electron-density maps obtained from the X-ray diffraction experiment allowed the authors to detect that the investigated crystal structure was composed of two different arylphorins: SP2 and SP3. This discovery was confirmed by N-terminal sequencing. SP2 has been extensively studied previously, whereas only a few reports on SP3 are available. However, to date no structural studies have been reported for these proteins. These studies revealed that SP2 and SP3 exist in the silkworm body as a heterohexamer formed by one SP2 trimer and one SP3 trimer. The overall fold, consisting of three haemocyanin-like subdomains, of SP2 and SP3 is similar. Both proteins contain a conserved N-glycosylation motif in their structures. © 2013 International Union of Crystallography.

Pietrzyk A.J.,Polish Academy of Sciences | Bujacz A.,Technical University of Lodz | Mueller-Dieckmann J.,University of Hamburg | Lochynska M.,Institute of Natural Fibers and Medicinal Plants | And 4 more authors.
PLoS ONE | Year: 2013

The 30-kDa family of lipoproteins from insect hemolymph has been the focus of a number of studies over the last few years. Recently, four crystal structures of Bombyx mori lipoprotein 7 have been determined. Here we report two crystal structures of another member of the 30-kDa lipoprotein family, Bombyx mori lipoprotein 3 (Bmlp3). The protein was isolated from its natural source, mulberry silkworm hemolymph. It crystallized in two different crystal forms, Bmlp3-p21 (space group P21) and Bmlp3-c2 (space group C2). The crystal structures were solved by molecular replacement using the coordinates of Bmlp7 as a starting model. The crystals of Bmlp3-p21 diffracted X-rays to 2.4 Å resolution and of Bmlp3-c2 to 2.1 Å resolution. Bmlp3 has an overall fold characteristic of 30-kDa lipoproteins, with a VHS-type N-terminal domain and β-trefoil C-terminal domain. Structural comparison of Bmlp3 and Bmlp7 shows that the loops present in the C-terminal domain are flexible and participate in dimer formation. Additionally, new putative binding sites of Bmlp3 have been analyzed in detail and the electrostatic potential of the protein surface at physiological pH 7.4 conditions has been calculated. The results of these calculations are the starting point for an explanation of the recently reported cell-penetrating properties of the 30-kDa lipoproteins. © 2013 Pietrzyk et al.

Czerny B.,Pomeranian Medical University | Krupka K.,Pomeranian Medical University | Ozarowski M.,Institute of Natural Fibers and Medicinal Plants | Seremak-Mrozikiewicz A.,Institute of Natural Fibers and Medicinal Plants
TheScientificWorldJournal | Year: 2014

BACKGROUND: Cancer constitutes a major health problem worldwide. Thus, search for reliable and practical markers of the disease process remains the key issue of the diagnostic process.OBJECTIVES: The study aims at linking the trace element status of an organism, assessed by hair analysis, with the occurrence of cancer diseases.MATERIAL AND METHODS: Hair samples were collected from 299 patients with cancer diseases confirmed by a histopathological test and from 100 controls. Cancer patients were divided into three groups, depending on cancer type: hormone-dependent cancer, cancer of the alimentary tract, and cancer with high glycolytic activity. Mineral element analysis of hair was performed using an atomic emission spectrophotometer with inductively coupled plasma (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS).RESULTS: Statistically significantly lower concentrations of selenium, zinc, copper, germanium and boron, iron, and magnesium were observed in the three groups of cancer patients. Disturbance in the axis glucose-insulin and changes in concentrations of heavy metals and toxic elements were also noted.CONCLUSIONS: It seems safe to conclude that our results confirmed usefulness of hair element analysis in screening tests for the assessment of the biomarker of various cancer diseases in a female population.

Pari L.,Italian Agricultural Research Council | Baraniecki P.,Institute of Natural Fibers and Medicinal Plants | Kaniewski R.,Institute of Natural Fibers and Medicinal Plants | Scarfone A.,Italian Agricultural Research Council
Industrial Crops and Products | Year: 2014

About two thousand species of plants are known in the world to yield natural fibers, but just a few are commercially important, providing nearly 90% of the global production of natural fibers.Since ancient times, humans required fiber to produce cordage, tools, clothes, nets, etc., in order to provide food and shelter.Among the most productive fiber plants, the bast fiber crops are undoubtedly considered as major contributors. The bast fibers are sclerenchyma fibers associated with the phloem of crops such as flax, hemp, kenaf, ramie, nettle, jute, etc.Currently, in both Europe and China, bast fibers are used for industrial applications in textile, construction, automotive, and other industries. Recently, bast fibers gained importance also as a renewable feedstock for the production of strong, lightweight, composite materials.However, there are factors in agro-industrial chain that limit the broad commercialization of these crops and their products. From an agronomical point of view, a part of the problems are associated with technological gaps in harvesting technologies, which impede the full exploitation of some crops. For instance, the production of high-quality fibers for textile firstly depends on the quality of the raw material, which in turn is linked, among the others, with the efficiency of the harvesting system adopted. In most cases, these systems were developed locally, based on available solutions in connection with specific local agricultural practice.The aim of this paper is to present a review of the existing mechanical harvesting systems for bast fiber crops in Europe and China, with special reference to hemp, flax, and kenaf. In addition, the work will furnish a description of the innovations that have been adopted in recent years to improve the harvesting processes in order to increase the value of these crops and their products. © 2014 Elsevier B.V.

Spychalski G.,Institute of Natural Fibers and Medicinal Plants
Journal of Natural Fibers | Year: 2014

The paper presents issues linked with the Polish sector of fibrous plants in terms of economic and production conditions. The author analyzes the situation of flax and hemp producers in which industrial plants have traditionally been cultivated in Poland. He determines the cause of significant reduction in the cultivation area of these plants in the total volume of crops, despite beneficial climatic and soil conditions prevailing in Poland. The paper also presents the economic conditions of the processes linked with agriculture of fibrous plants with special reference to globalization phenomena and current situation in the European Union. Further the paper shows the results of the study carried out among the farmers working in Wielkopolska region of Poland. Their responses indicate that the cultivation of fibrous plants is a marginal form of agricultural activity and it is believed to be difficult and the demand for the produce is not guaranteed. The farmers also would welcome higher economic effectiveness of these crops that could result from agricultural policy instruments, i.e., subsidies to the production of the fibrous raw materials. The paper concludes with the description of the perspectives for the development of this sector of agriculture and possible nontextile applications of the flax and hemp, e.g., in biomedicine, construction, and energy production. Copyright © Taylor & Francis Group, LLC.

Abdel-Halim E.S.,National Research Center of Egypt | Konczewicz W.,Institute of Natural Fibers and Medicinal Plants | Zimniewska M.,Institute of Natural Fibers and Medicinal Plants | Al-Deyab S.S.,King Saud University | El-Newehy M.H.,King Saud University
Carbohydrate Polymers | Year: 2010

Bioscouring of flax fabrics was carried out using combinations of different pectinase preparations and different surfactants having varying ionic natures. In order to attain satisfactory fabric hydrophilicity, the enzymatic scouring step was necessary to be followed by emulsification post-treatment, in which the temperature of the bioscouring liquor was raised to 90 °C for 2 min. Flax fabrics subjected to such combined treatments (bioscouring followed by emulsification post-treatment) were evaluated by measuring their physico-chemical properties, morphological features and metrological parameters and comparing them with the corresponding properties recorded for the grey flax fabric. The measurements showed high improvement in the water absorbency and percent moisture regain of the treated fabrics. The physico-chemical properties, morphological structure and metrological parameters of the treated flax fabrics were found to be dependent on both the efficiency of the enzyme preparation and the ionic nature of the used surfactant. © 2010 Elsevier Ltd. All rights reserved.

Loading Institute of Natural Fibers and Medicinal Plants collaborators
Loading Institute of Natural Fibers and Medicinal Plants collaborators