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Lushnikova E.L.,Institute of Molecular Pathology and Pathomorphology
Bulletin of Experimental Biology and Medicine | Year: 2015

The relationship between vascular endothelium growth factor (VEGF) and cardiomyocyte oxidative phosphorylation level in experimental myocardial infarction, caused by diathermocoagulation of the periconical ventricular artery, was studied by immunofluorescent microscopy. Staining showed uneven distribution of cytochrome c in the mitochondria in the focus of myocardial infarction 2 h after the operation. After 24 h uneven staining of cardiomyocytes was found in the peri-infarction zone and no staining in the zone of myocardial infarction. This indicated a significant decrease in the level of redox enzymes. This picture persisted till day 14. Intraventricular injection of VEGF to animals led to a significant increase of the immunohistochemical reaction intensity, which reached the peak by day 7. The distribution of immunohistochemical reaction products under conditions of VEGF blocking was about the same as in spontaneous postinfarction reparative restitution. Our data indicated that increase of VEGF concentration in the myocardium maintained and stimulated oxidative phosphorylation in cardiomyocytes during the postinfarction period. © 2015, Springer Science+Business Media New York. Source


Morozkova T.S.,Institute of Molecular Pathology and Pathomorphology | Kaledin V.I.,Russian Academy of Sciences
Bulletin of Experimental Biology and Medicine | Year: 2015

Experiments on male and female CC57BR/Mv mice differing by the sensitivity to carcinogenic effect of urethane on the lungs showed that castration 1 week before carcinogen challenge reduced the number of lung adenomas caused by it in males and somewhat increased the number of tumors in females. Exogenous testosterone after urethane injection caused virtually no changes in urethane effect in males and females. By contrast, elevation of testosterone concentrations in newborn male and female mice by injections or its decrease in feminized males receiving sodium glutamate during the neonatal period reduced the sensitivity to the carcinogenic effect of urethane in adult males and to its increase in females. © 2015, Springer Science+Business Media New York. Source


Voropaeva O.F.,Russian Academy of Sciences | Shokin Yu.I.,Russian Academy of Sciences | Nepomnyaschchikh L.M.,Institute of Molecular Pathology and Pathomorphology | Senchukova S.R.,Institute of Molecular Pathology and Pathomorphology
Proceedings - 2015 International Conference on Biomedical Engineering and Computational Technologies, SIBIRCON 2015 | Year: 2015

This paper is devoted to a numerical analysis of the solutions of the equations system describing the dynamics of the concentration of p53 and Mdm2 proteins in their interaction. A detailed study of the solutions was made when the mathematical model parameters deviated from the basal values. Within the numerical experiments, the states of the system, such as threat by the uncontrolled apoptotic death of cellules, accelerating the aging processes of organisms and of the excessive suppression of p53-induced apoptosis, which increases the risk of tumors, have been investigated. The mechanism of managing the system under stress conditions has been studied in detail. © 2015 IEEE. Source


Kisarova Y.A.,Research Institute of Physiology and Fundamental Medicine | Kaledin V.I.,RAS Institute of Cytology and Genetics | Bogdanova L.A.,Institute of Molecular Pathology and Pathomorphology | Korolenko T.A.,Research Institute of Physiology and Fundamental Medicine
Bulletin of Experimental Biology and Medicine | Year: 2015

Ratio between proMMP and active MMP was studied in the dynamics of growth of the Lewis lung adenocarcinoma with lung metastasis. It was shown that tumor growth is associated with an increase in the content of proMMP (day 20; terminal stage), but the level of active MMP in tumor tissue did not significantly change. The development of lung metastasis was accompanied by accumulation of active MMP (days 7, 15, and 20) and a decrease in the content of pro-MMP (days 7, and 20) in comparison with the control. In the spleen of these mice (metastasis-free organ), an increase in the levels of proMMP (day 20) and especially active MMP (days 7, 15, and 20) were found. The results suggest that tumor development shifts the proportion between active MMP and proenzymes in the tumor, lungs with metastasis, and spleen without metastasis. © 2015 Springer Science+Business Media New York. Source


Kaledin V.I.,RAS Institute of Cytology and Genetics | Nikolin V.P.,RAS Institute of Cytology and Genetics | Popova N.A.,Novosibirsk State University | Pyshnaya I.A.,Russian Academy of Sciences | And 2 more authors.
Bulletin of Experimental Biology and Medicine | Year: 2015

Antitumor effect of paclitaxel used as the monotherapy or in combination with cyclophosphamide was studied on CBA/LacSto mice with transplanted LS and RLS tumors characterized by high (LS) and low (RLS) sensitivity to cyclophosphamide. The therapeutic effects of cyclophosphamide and paclitaxel were summed in animals with drug-resistant RLS tumor, while combined use of these drugs in LS tumor highly sensitive to the apoptogenic effect of cyclophosphamide was no more effective than cyclophosphamide alone. © 2015, Springer Science+Business Media New York. Source

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