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PubMed | Institute of Molecular Pathology and Pathomorphology and Institute of Basic and Clinical Immunology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The effects of recombinant analog of natural Ebola virus protein vp24 in configurations virulent (vp24-ad) and avirulent (vp24-w) for guinea pigs on interferonogenesis were studied in vivo and in vitro. Amino acid differences were determined by His186 substitution in avirulent (nonlethal) configuration for Tyr in the virulent (lethal) one. Recombinant analogs vp24-w and vp24-ad inhibited interferonogenesis in vivo and in vitro. Inhibition by the two protein configurations was virtually the same.


PubMed | Institute of Physiology and Basic Medicine, Novosibirsk State Medical University, Institute of Molecular Pathology and Pathomorphology and University Canada West
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Suppression of functional activity of macrophages by gadolinium chloride, suppressing the macrophage population and the endocytosis velocity, was studied in vivo. Injection of GdCl3 led to an increase in serum cholesterol concentration. Preliminary injection of GdCl3 to mice with lipidemia 24 h before poloxamer 407 reduced the concentrations of triglycerides and LDL during marked depression of macrophages (in 24 h). Macrophage repopulation (days 5, 7) was associated with development of a trend to an increase of triglyceride and LDL levels. lectron microscopic study of Kupffer cells after injection of poloxamer 407 and its combination with gadoliniun chloride detected the intralysosomal accumulation syndrome in these cells (formation of auto- and heterophagolysosomes). Activity of cathepsin B, characteristic of macrophages, reduced 24 h after injections of GdCl3 and poloxamer 407 alone and restored in response to their combination.


PubMed | Institute of Molecular Pathology and Pathomorphology, Altai State Medical University and Research Institute of Clinical and Experimental Lymphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Morphological and ultrastructural study of the kidney was performed in rats with oxalate nephrolithiasis. Specific features of endoplasmic reticulum stress were evaluated during nephrolithiasis and treatment with -tocopherol. We observed the signs of endoplasmic reticulum stress with activation of proapoptotic pathways and injury to the cell lining in nephron tubules and collecting ducts. Ultrastructural changes were found in the organelles, nuclei, and cell membranes of epitheliocytes. A relationship was revealed between endoplasmic reticulum stress and oxidative damage, which developed at the early state of lithogenesis.


PubMed | Institute of Molecular Pathology and Pathomorphology and RAS Institute of Cytology and Genetics
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Experiments were performed on the model of transplanted mouse tumor with high incidence of liver metastases. Hydrophilic drug cycloplatam (injected intravenously in liposomes) was more potent than free cycloplatam (injected intravenously or intraperitoneally in physiological saline) in inhibiting the growth of natural and experimental metastases in the liver. By contrast, liposomal cycloplatam had lower efficiency than free cycloplatam in suppressing the growth of solid tumor. Liposomal and free cortifen (hydrophobic hormonal cytostatic) produced nearly the same effects on solid tumor growth. Our results suggest that liposomal forms of hydrophobic compounds producing nonselective effect on tumor cells (e.g., actinomycin D or Cosmegen), should not have advantages over free forms.


PubMed | Institute of Molecular Pathology and Pathomorphology, RAS Institute of Cytology and Genetics and Research Institute of Molecular Biology and Biophysics
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The general toxic and hepatocarcinogenic effects of diethylnitrosamine after stimulation of its metabolism with 1,4-bis[2-(3,5-dichloropyridyloxy)]-benzene (TCPOBOP) were studied. The hydroxylating activity of liver microsomes of C57Bl/6Mv mice towards p-nitrophenol increased more than 4-fold 3 days after injection of TCPOBOP. Injection of diethylnitrosamine 3 days after TCPOBOP caused a lesser body weight loss and decrease of food consumption in C57Bl/6Mv mice than in response to diethylnitrosamine without preinduction. Injection of diethylnitrosamine to suckling ICR mice after TCPOBOP induction of cytochrome P450 2e1 activity led to development of 2-fold lesser number of tumors and pretumorous nodes in the liver in comparison with animals injected with diethylnitrosamine without induction. These data indicated that metabolism stimulation reduced the general toxic and hepatocarcinogenic effects of diethylnitrosamine.


Morozkova T.S.,Institute of Molecular Pathology and Pathomorphology | Kaledin V.I.,Russian Academy of Sciences
Bulletin of Experimental Biology and Medicine | Year: 2015

Experiments on male and female CC57BR/Mv mice differing by the sensitivity to carcinogenic effect of urethane on the lungs showed that castration 1 week before carcinogen challenge reduced the number of lung adenomas caused by it in males and somewhat increased the number of tumors in females. Exogenous testosterone after urethane injection caused virtually no changes in urethane effect in males and females. By contrast, elevation of testosterone concentrations in newborn male and female mice by injections or its decrease in feminized males receiving sodium glutamate during the neonatal period reduced the sensitivity to the carcinogenic effect of urethane in adult males and to its increase in females. © 2015, Springer Science+Business Media New York.


PubMed | Institute of Molecular Pathology and Pathomorphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

The influence of Opisthorchis felineus invasion on the development of pathological changes in the hepatobiliary system was studied in 120 golden hamsters in a long-term experiment (42 weeks) after single infection per os in the dose of 50 metacercariae per animal. The animals were sacrificed on weeks 4, 8, 12, 16, 28 and 42. Chronic experimental infestation with O. felineus triggered a cascade of morphogenetic processes in both extrahepatic and intrahepatic biliary systems. At the early stages of the experiment, polyps and strictures of bile ducts were formed in the lobar bile ducts; in portal tracts, hyperplasia and adenomatous transformation of the newly formed epithelial structures were observed. At the later stages, third-degree biliary intraepithelial neoplasia developed in the lobar bile ducts; in the intrahepatic bile ducts, increased epitheliocyte hyperplasia and invasive growth of cell cords were observed, that impaired tissue architectonics. Progressing cell atypia can be classified as cholangiocellular cancer.


PubMed | Institute of Molecular Pathology and Pathomorphology
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

Clinical-morphological study of myelofibrosis was carried out in patients with chronic lymphocytic leukemia at the debut of the disease. Trephinobiopsy specimens of the ileac bone, aspirated specimens of the bone marrow, and peripheral blood smears were studied in 80 patients. Chronic lymphocytic leukemia was associated with myelofibrosis of different severity in 22.5% cases. Morphometric analysis of trephinobiopsy specimens showed that the severity (histology and dissemination) of myelofibrosis correlated with the type of tumor involvement of the bone marrow. Focal tumor involvement of the bone marrow predominated in trephinobiopsy specimens from patients without myelofibrosis, while patients with myelofibrosis developed mainly diffuse tumor infiltration, associated with the greatest dissemination of the initial and manifest myelofibrosis. No myelofibrosis was found in patients with interstitial tumor involvement of the bone marrow. The severity of the initial and manifest myelofibrosis directly correlated with the tumor involvement of the bone marrow and peripheral blood. Evaluation of the prognosis showed that initial myelofibrosis was associated with disease standing of 5.5 months, while manifest condition with a disease of 8.5 months and longer.


PubMed | Institute of Molecular Pathology and Pathomorphology, Research Institute of Chemical Diversity and RAS Nikolaev Institute of Inorganic Chemistry
Type: Journal Article | Journal: Bulletin of experimental biology and medicine | Year: 2016

In rats with obesity induced by high fat ration, the concentrations of K, Ca, Mn, Fe, Cu, Zn, Se, Br, Rb, and Sr were measured in the liver and lungs using synchrotron X-ray fluorescence analysis. Recombinations of inter-element correlations in the liver and lungs of rats receiving or not receiving additional Zn were observed under fasting conditions and after eating lard. Sr and Ca made the most pronounced contribution in the redistribution of correlations in the liver of obese rats. In contrast to other bioelements, Sr had negative correlations with all study elements in the liver of obese rats during physiological starvation. Long-term administration of Zn to rats was followed by recombination of both fasting and postprandial inert-element correlations in the liver and lungs, but Zn concentration did not change under these conditions. Redistribution of correlations in the liver and lungs of obese rats under fasting conditions reflects fundamental changes in metabolic processes, while redistribution observed after fat meal indicates temporary (postprandial) changes.

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