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Das B.,Institute of Mineral and Material Technology
Agris On-line Papers in Economics and Informatics | Year: 2012

Leaves of Tagetes erecta and cannabis sativa traditionally practiced in the treatments of boils, dysentery, indigestion and various skin infections. The aim of this study was on the one hand to extract crude alkaloid from the leaves of Tagetes and Cannabis plants and on the other hand to evaluate the in vitro antibacterial activities of the crude extract against bacterial strains representative of skin, mouth and ear microflora and also against β-strain of E. coli bacteria responsible for urinary tract infection in human beings by disc diffusion method. The active ingredient of Tagetes erecta and Cannabis sativa was identified as alkaloid by Mayer's and Dragendorff's test. The antimicrobial activities were compared with two standard anbiotics Chloramphenicol and Tetracycline.However, this study revealed maximum growth inhibition and effectiveness was remarkably observed in the extracts of Cannabis sativa and then in Tagetes erecta. These results indicate that leaves have a potential broad spectrum antibacterial activity. © 2010.

Tripathy A.,Regional Medical Research Center Indian Council of Medical Research | Samanta L.,Ravenshaw University | Das S.,Ravenshaw University | Parida S.K.,Regional Medical Research Center Indian Council of Medical Research | And 5 more authors.
Journal of Medical Entomology | Year: 2011

Larvicidal activity of methanolic plant extracts of Lantana cramera (P1) root and Anacardium occidentale (P2) leaf was investigated against the larvae of the three mosquito species (Culex quinquefasciatus, Anopheles stephensi, and Aedes aegypti reared in the laboratory), and the respective glutathione S-transferase (GST) activity was analyzed as an index of protection against the extracts. The LC50 (extract concentration that shows 50% mortality) values of Pl extract for An. stephensi, Ae. aegypti, and Cx. quinquefasciatus were 132.55,27.82, and 11.68 ppm, respectively, whereas those of P2 extract were 56.81, 912, and 10.79 ppm, respectively. In general, in the untreated groups, the level of GST activity was significantly higher in Ae. aegypti in comparison with An. stephesi and Cx. quinquefasciatus. However, the enzyme activity failed to show any response when treated with either of the plant extracts in Ae. aegypti. However, an increase in the GST activity was recorded in extract-treated larvae of both An. stephensi and Cx. quinquefasciatus. The results of the current study suggest that both the plant extracts show species-specific mosquitocidal potential. Induction of GST activities in survived An. stephensi and Cx. quinquefasciatus larvae suggests the role of this enzyme in conferring resistance to the plant extracts. © 2011 Entomological Society of America.

Das N.,Institute of Mineral and Material Technology | Das R.,Institute of Mineral and Material Technology | Das S.N.,Institute of Mineral and Material Technology | Swamy Y.V.,Indian Institute of Chemical Technology | And 2 more authors.
Atmospheric Research | Year: 2011

Suspended particulate matter (SPM) was collected by using high volume samplers (HVS) from two different locations: Bay of Bengal (BoB) and State Botanical Garden (SBG) situated in the eastern part of India. The SPM samples were collected on Whatman No.41 filter papers. The samples were simultaneously collected daily from both the locations during March 17-April 1, 2006. The average SPM concentration over BoB and SBG was found to be 39.05 and 78.14μg/m3 respectively. Na+ and SO4 2- were the most dominant water-soluble constituents over BoB and SBG respectively. The ratio of SO4 2-/NO3 - concentrations in both the cases was observed to be >1 indicating that the contribution of atmospheric acidity was more due to SO4 2- than NO3 -. From intra-correlation analysis it is found that at both the locations, Na+ and Cl- were well correlated while SO4 2--Ca2+ and SO4 2--NH4 + were poorly correlated indicating the later ion pairs to be derived from different sources. The Windrose diagrams for wind speed and direction show that for SBG, the wind blew from the SE and SW directions and brought in pollutants from soil and anthropogenic sources. In case of BoB, the wind parcels mostly were blown from the land mass (EES direction) showing the influence of anthropogenic pollutants on the marine aerosols. All the variables can be classified into three factors using principal component analyses technique. It explained 85% of total variance. © 2010 Elsevier B.V.

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