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Zhang J.,PLA Fourth Military Medical University | Huang C.-L.,Institute of Military Training Related Medical science
Medical Journal of Chinese People's Liberation Army | Year: 2015

Objective To investigate the effects of percutaneous midband pulse current stimulation in hepatic region on anti-exercise fatigue ability and the free radicals and nissl bodies in cerebral cortex tissue of rats with exercise-induced fatigue. Methods Seventy-two 8-week old male Wistar rats were randomly divided into 4 groups (18 each): control group (CG), fatigue group (FG), stimulation before fatigue group (SBF) and stimulation after fatigue group (SAF). Animals in FG, SBF and SAF group were used to reproduce the swimming-exhaustion models. Midband current stimulation (1024Hz, 10mA, current cycle 1sec) for 20 minutes was given to the rats of group SBF before swimming, and to those in group SAF after exhaustion. At the weekend of the 1st, 3rd and 5th week after modeling, the exhaustive swimming time of rats in all but CG group was observed. Cerebral cortex tissue was harvested for the estimation of the level of lipid peroxidation, including SOD, MDA, GSH-Px and SOD/MDA, and the histopathological changes in nissl bodies in neurons were observed. Results At the 1st weekend after modeling, no significant difference was found in all the indexes among the 4 groups, while at the 3rd weekend, the exhaustive time was obviously longer in SAF group than in FG group, and also in SAF group than in FG and SBF group at the 5th weekend (P<0.05). At the 5th weekend, the SOD and GSH-Px levels and SOD/MDA contents were obviously lower in FG and SBF group than in CG and SAF group, and the MDA content was obviously higher in FG and SBF group than in CG and SAF group (P<0.05). As regarding the nissl bodies in neurons, it is observed that the ratio of number/area was obviously higher in SAF group than in FG and SBF group at the 5th weekend (P<0.01). Conclusion Percutaneous stimulation of hepatic region with midband pulse current can effectively reduce the lipid peroxidation damage of cerebral cortex tissue and decrease the dissolution and loss of nissl bodies in fatigued rats, prolong the exhausting exercise time, and postpone the development of fatigue. © 2015, People’s Military Medical Press. All rights reserved. Source


Ge W.,PLA Fourth Military Medical University | Mu J.,PLA Fourth Military Medical University | Huang C.,Institute of Military Training Related Medical science
International Journal of Sports Medicine | Year: 2014

Genetic factor have previously been shown to play an important role in sports injuries and recovery. GDF5 Single-Nucleotide Polymorphism rs143383 has been recently reported to be associated with fracture susceptibility. Furthermore, the effect of GDF5 during the recovery processes of trauma is increased. In the present study, we aimed to evaluate whether this SNP was associated with susceptibility to the meniscus injury and postoperative recovery in Chinese male soldiers. GDF5 SNP was genotyped in 135 male soldiers with meniscus injury and 400 healthy male controls. Moreover, the function recovery of the soldiers suffering from the meniscal repair was also assessed. Our data showed that the GDF5 TT genotype (60.0 vs. 47.25%; P=0.010) and T allele (76.3 vs. 68.75%; P=0.019) were significantly over-represented in the meniscus injury group compared with the control group. We found that the TC (P<0.05), CC (P<0.05) and C carriers (P<0.05) genotype exhibited significantly higher Lysholm Scores than the TT genotype at 1 month postoperative. In addition, the CC (P<0.05) genotype also demonstrated significantly higher Lysholm Scores than the TT genotype 2 months postoperative. Taken together, our results revealed that the GDF5 SNP was associated with susceptibility to the meniscus injury and postoperative function recovery in Chinese male soldiers. © Georg Thieme Verlag KG Stuttgart New York. Source


Zhang D.H.,Institute of Military Training Related Medical science
Orthopaedic surgery | Year: 2011

To investigate the diagnosis and surgical treatment of excessive lateral pressure syndrome of the patellofemoral joint caused by military training. Fifteen patients (patient group) and 18 healthy volunteers (control group) were involved in this retrospective study. Radiographs of the knee joints of all patients and volunteers were taken. The bone architecture was assessed, the trochlear angle, coincidence angle and patellofemoral joint index measured in both groups, and the resulting data compared. All 15 patients (17 knees) were treated by lateral collateral retinaculum release. Pre- and post-operative pain was evaluated with a visual analog scale (VAS). The differences between the two groups in coincidence angle (patient group: 7.67°± 5.81°; control group: -2.2°±-2.71°) and patellofemoral joint index (patient group: 2.49 ± 1.40; control group: 1.25 ± 0.15) were statistically significant. Subchondral bone sclerosis and osteophytosis in the patellofemoral joint were more pronounced in the patient group than in the control group. The VAS was higher preoperatively (7.06 ± 0.85) than postoperatively (6 months postoperatively: 3.87 ± 0.24; 1 year postoperatively: 3.01 ± 0.17), and the differences between preoperative and postoperative were statistically significant. Apart from the case history, typical symptoms and physical signs, X-ray examination is the most basic way to diagnose excessive lateral pressure syndrome of the patellofemoral joint, and the patellofemoral joint index is the most reliable for diagnosis. Lateral collateral retinaculum release with a small-incision is an effective treatment for this disease. © 2011 Tianjin Hospital and Blackwell Publishing Asia Pty Ltd. Source


Shang X.,PLA Fourth Military Medical University | Huang C.,Institute of Military Training Related Medical science | Chang Q.,Institute of Military Training Related Medical science | Zhang L.,Institute of Military Training Related Medical science | Huang T.,Institute of Military Training Related Medical science
International Journal of Sports Medicine | Year: 2010

The α-actinin 3 (ACTN3) gene is absent in 18% of healthy white individuals owing to homozygosity for a premature stop codon polymorphism (rs1815739) and is only expressed in the Z line of fast glycolytic muscle fibres. Previous studies have shown highly significant association between ACTN3 genotype and sprint/power performance, while the nonfunctional allele (577X) was believed to provide an advantage for endurance performance. In this study we tested whether XX genotype was over-represented in Chinese endurance athletes compared to the general population. In a study of 250 Chinese endurance athletes of provincial or national competitive standard and 450 controls, we proved that the ACTN3 XX genotype (21.2 vs. 15.8%; P=0.02) and X allele (51.3 vs. 41.1%; P=0.019) were significantly over-represented in female endurance athletes compared to controls, while no genotype-related differences were observed in male endurance athletes. Besides, the frequency of the ACTN3 XX genotype (28.6%) was the highest in a group of highly elite athletes compared with other groups, which supported the hypothesis that the absence of -actinin-3 provided some sort of advantage for endurance athletes. Our results indicated that ACTN3 R577X polymorphism was associated with endurance performance in female athletes but not male athletes in China. © Georg Thieme Verlag KG Stuttgart - New York. Source


Mu J.,425th Hospital of Chinese PLA | Ge W.,534th Hospital of Chinese PLA | Zuo X.,Institute of Military Training Related Medical science | Chen Y.,Institute of Military Training Related Medical science | Huang C.,Institute of Military Training Related Medical science
European Spine Journal | Year: 2014

Purpose: The aim of the present study is to assess whether the single nucleotide polymorphism in the GDF5 (+104T/C; rs143383) is associated with the symptomatic lumbar disc herniation in the Chinese Han population and the identification of the mechanisms of its action. Methods: This study consisted of 231 patients with symptomatic lumbar disc herniation as the case group and 370 patients who had a lifetime lack of symptoms as the control group. PCR products were genotyped. Thirty-eight disc specimens derived from the cases were analyzed by immunohistochemical staining. The stain intensity of immunohistochemistry was quantified using a computerized image analysis system. Results: Significant differences in genotypic and allelic frequencies were found between case group and control group (TT genotype P < 0.001; CC genotype P = 0.002; T allele P < 0.001). The T allele was more frequent in the case group regardless of gender (Female P = 0.018; Male P < 0.001). Significant differences were found in the genotype frequencies when stratified by gender except the comparison between the CC genotype and other genotypes combined among the female samples (P > 0.05). A semi-quantification of collagen protein in the nucleus pulposus showed that the average collagen protein content in TC group was higher than in TT group (P < 0.05). Conclusion: Our results suggested that the GDF5 polymorphism is associated with a susceptibility to symptomatic lumbar disc herniation in the Chinese Han population and type II collagen in the nucleus pulposus may be a key factor in susceptibility to symptomatic lumbar disc herniation. © 2013 Springer-Verlag. Source

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