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Liu J.,Shanghai JiaoTong University | Nie H.,Nanchang University | Xu Z.,Shanghai JiaoTong University | Guo F.,Nanchang University | And 7 more authors.
Journal of Materials Chemistry B | Year: 2014

Platelet-rich plasma (PRP) has been widely used for decades in the clinic, since an abundance of growth factors can be released when it is activated. However, its clinical use is limited because the release of growth factors is temporal and PRP lacks mechanical strength. The aim of this study was to incorporate PRP-derived growth factors into PCL/gelatin nanofibers using the emulsion electrospinning method to determine how growth factors are released from the scaffolds and how the presence of these factors enhances the bioactivity of the scaffolds. Scaffolds with or without PRP were prepared and characterized. The release of proteins from scaffolds over time and rabbit BMSC chemotaxis, proliferation, and chondrogenic induction were quantified in vitro. The in vivo restoring effect of the scaffolds was also evaluated by transplanting the scaffolds into a cartilage defect in an animal model, and the outcomes were determined by histological assessment, micro-CT scanning, and IL-1 measurement. The results showed that the mechanical properties of the scaffolds were mildly compromised due to the addition of PRP, and that the sustained release of growth factors from PRP-containing scaffolds occurred up to ∼30 days in culture. The scaffold bioactivity was enhanced, as BMSCs demonstrated increased proliferation and notable chemotaxis in the presence of PRP. Chondrogenesis of BMSCs was also promoted when the cells were cultured on the PRP scaffolds. Furthermore, the PRP scaffolds showed better restorative effects on cartilage defects, as well as anti-inflammatory effects in the joint cavity (the IL-1 level was decreased). In conclusion, the results of the current study indicate the potential for using a PRP-containing electrospun nanofibrous scaffold as a bioactive scaffold, which is beneficial for optimizing the clinical application of PRP. This journal is © The Royal Society of Chemistry. Source


Guan J.-J.,Shanghai JiaoTong University | Guan J.-J.,Institute of Microsurgery on Extremities | Niu X.,Shanghai JiaoTong University | Niu X.,Institute of Microsurgery on Extremities | And 10 more authors.
Tissue Engineering - Part A | Year: 2014

Stem cells in human urine have gained attention in recent years; however, urine-derived stem cells (USCs) are far from being well elucidated. In this study, we compared the biological characteristics of USCs with adipose-derived stem cells (ASCs) and investigated whether USCs could serve as a potential cell source for neural tissue engineering. USCs were isolated from voided urine with a modified culture medium. Through a series of experiments, we examined the growth rate, surface antigens, and differentiation potential of USCs, and compared them with ASCs. USCs showed robust proliferation ability. After serial propagation, USCs retained normal karyotypes. Cell surface antigen expression of USCs was similar to ASCs. With lineage-specific induction factors, USCs could differentiate toward the osteogenic, chondrogenic, adipogenic, and neurogenic lineages. To assess the ability of USCs to survive, differentiate, and migrate, they were seeded onto hydrogel scaffold and transplanted into rat brain. The results showed that USCs were able to survive in the lesion site, migrate to other areas, and express proteins that were associated with neural phenotypes. The results of our study demonstrate that USCs possess similar biological characteristics with ASCs and have multilineage differentiation potential. Moreover USCs can differentiate to neuron-like cells in rat brain. The present study shows that USCs are a promising cell source for tissue engineering and regenerative medicine. © Copyright 2014, Mary Ann Liebert, Inc. 2014. Source

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