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Pham T.L.,International University | Trinh T.T.,Institute of Microbiology and Biotechnology | Nguyen H.T.,International University
IFMBE Proceedings | Year: 2015

This study aimed to evaluate the antimicrobial activity of Senna alata (L.), Rhinacanthus nasutus and Chromolaena odorata against antibiotic resistant Staphylococcus aureus, Pseudomonas aeruginosa clinical strains; vitro – induced antibiotic resistant S. aureus strains; S. aureus ATCC 25213 and P. aeruginosa ATCC 9027. Plant samples were extracted with different solvents including ethanol, ethyl acetate and n-hexane. Disc diffusion method was used to evaluate the antimicrobial activity of extracts. In general, most of extracts showed good antimicrobial activity against both antibiotic resistant and sensitive S. aureus but no activity against P. aeruginosa. To clinical S. aureus strains, C. odorata and R. nasutus extracts using ethyl acetate showed better inhibitory zones (17.17 ± 0.29 mm; 16.67 ± 0.58 mm) than the ones extracted using ethanol (14.17 ± 0.58mm, 11.67 ± 1.53 mm) and n- hexane (12.17 ± 0.58 mm; 13.67 ± 0.58 mm, respectively). To other S. aureus strains, S. alata (L.) extract using ethanol showed the greatest inhibition zone (22.33 ± 0.58 mm). This result suggested that these traditional herbs should be further investigated to serve as alternative treatment for multidrug- resistant S. aureus infections and probably also for other gram positive pathogenic bacteria. © Springer International Publishing Switzerland 2015.

Singh T.P.,National Dairy Research Institute | Kaur G.,National Dairy Research Institute | Malik R.K.,National Dairy Research Institute | Schillinger U.,Institute of Microbiology and Biotechnology | And 2 more authors.
Probiotics and Antimicrobial Proteins | Year: 2012

This study was conducted to evaluate the probiotic properties of Lactobacillus reuteri isolated from human infant feces (less than 3 months). Out of thirty-two representative L. reuteri strains isolated from the infant human feces, nine isolates (i.e. LR5, LR6, LR9, LR11, LR19, LR20, LR25, LR26 and LR34) showed survival in acid, bile and simulated stomach-duodenum passage conditions, indicating their high tolerance to gastric juice, duodenal juice and bile environments. The nine isolates did not show strong hydrophobic properties because the percentages of adhesion to the apolar solvent, n-hexadecane, did not exceed 40%, showing that their surfaces were rather hydrophilic. Functionality of these nine probiotic isolates was supported by their antagonistic activity and their ability to deconjugate bile salts. The safety of the nine indigenous L. reuteri isolates was supported by the absence of transferable antibiotic resistance determinants, DNase activity, gelatinase activity and hemolysis. The results obtained so far suggest that the nine strains are resistant to low pH, bile salts and duodenum juice, so they could survive when passing through the upper part of the gastrointestinal tract and fulfill their potential probiotic action in the host organism. According to these results, the L. reuteri strains isolated from human infant feces possess interesting probiotic properties that make them potentially good candidates for probiotics. © 2012 Springer Science + Business Media, LLC.

Mihatsch W.A.,Deaconry Hospital | Vossbeck S.,Section of Neonatology and Pediatric Critical Care | Eikmanns B.,Institute of Microbiology and Biotechnology | Hoegel J.,University of Ulm | Pohlandt F.,Section of Neonatology and Pediatric Critical Care
Neonatology | Year: 2010

Background: Nosocomial infections endanger preterm infants. Objective: The aim of the present controlled randomized trial was to investigate whether Bifidobacterium lactis reduces the incidence of nosocomial infections in infants with very low birth weight (VLBW; <1,500 g) <30 weeks of gestation. Patients and Methods: In a randomized controlled trial, 183 VLBW infants <30 weeks of gestation were stratified according to gestational age (23-26 and 27-29 weeks) and early antibiotic therapy (days 1-3, yes or no) and randomly assigned to have their milk feedings supplemented with B. lactis (6 × 2.0 × 109 CFU/kg/day, 12 billion CFU/kg/day) or placebo for the first 6 weeks of life. Primary outcome was the 'incidence density' of nosocomial infections defined as periods of elevated C-reactive protein (>10 mg/l) from day 7 after initiation of milk feedings until the 42nd day of life (number of nosocomial infections/total number of patient days). The main secondary outcome was necrotizing enterocolitis (NEC; ≥stage 2). Results: There were 93 infants in the B. lactis group and 90 in the placebo group. There was no significant difference between the two groups with regard to the incidence density of nosocomial infections (0.021 vs. 0.016; p = 0.9, χ2 test). There were 2 cases of NEC in the B. lactis group and 4 in the placebo group. None of the blood cultures grew B. lactis. Conclusion: In the present setting, B. lactis at a dosage of 6 × 2.0 × 109 CFU/kg/day (12 billion CFU/kg/day) did not reduce the incidence density of nosocomial infections in VLBW infants. No adverse effect of B. lactis was observed. Copyright © 2010 S. Karger AG, Basel.

Henry M.,Cork Institute of Technology | Begley M.,University College Cork | Neve H.,Institute of Microbiology and Biotechnology | Maher F.,Cork Institute of Technology | And 4 more authors.
FEMS Microbiology Letters | Year: 2010

In this study, we describe the characterization, cloning, expression and purification of the lysin A gene of the mycobacteriophage TM4. The gene TM4-gp29 (gp29) is a 1644-bp gene that codes for a 58.6-kDa protein and contains peptidoglycan recognition protein, Zn-binding and amidase catalytic domains. The gene was cloned into Escherichia coli using the 'His-Tag' pQE60 vector. After affinity chromatography-mediated purification, the protein was concentrated and visualized using sodium dodecyl sulphate polyacrylamide gel electrophoresis. Evidence of peptidoglycan-degrading activity was observed initially by a chloroform assay and later by conventional zymogram analysis. © 2010 Federation of European Microbiological Societies.

Hoyles L.,University College Cork | McCartney A.L.,University of Reading | Neve H.,Institute of Microbiology and Biotechnology | Gibson G.R.,University of Reading | And 4 more authors.
Research in Microbiology | Year: 2014

This work represents an investigation into the presence, abundance and diversity of virus-like particles (VLPs) associated with human faecal and caecal samples. Various methodologies for the recovery of VLPs from faeces were tested and optimized, including successful down-stream processing of such samples for the purpose of an in-depth electron microscopic analysis, pulsed-field gel electrophoresis and efficient DNA recovery. The applicability of the developed VLP characterization method beyond the use of faecal samples was then verified using samples obtained from human caecal fluid. © 2014 Institut Pasteur.

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