Julich S.,Friedrich Loeffler Institute |
Kopina R.,Institute of Microbial science and Technologies Ltd. |
Kopina R.,Institute of Metagenomics and Microbial Technologies |
Hlawatsch N.,Microfluidic ChipShop GmbH |
And 4 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2014
Lab-on-a-chip systems are innovative tools for the detection and identification of microbial pathogens in human and veterinary medicine. The major advantages are small sample volume and a compact design. Several fluidic modules have been developed to transform analytical procedures into miniaturized scale including sampling, sample preparation, target enrichment, and detection procedures. We present evaluation data for single modules that will be integrated in a chip system for the detection of pathogens. A microfluidic chip for purification of nucleic acids was established for cell lysis using magnetic beads. This assay was evaluated with spiked environmental aerosol and swab samples. Bacillus thuringiensis was used as simulant for Bacillus anthracis, which is closely related but non-pathogenic for humans. Stationary PCR and a flow-through PCR chip module were investigated for specific detection of six highly pathogenic bacteria. The conventional PCR assays could be transferred into miniaturized scale using the same temperature/time profile. We could demonstrate that the microfluidic chip modules are suitable for the respective purposes and are promising tools for the detection of bacterial pathogens. Future developments will focus on the integration of these separate modules to an entire lab-on-a-chip system. © 2014 SPIE.
Kovac Virsek M.,Institute of Microbial science and Technologies Ltd. |
Hubad B.,Institute of Microbial science and Technologies Ltd. |
Lapanje A.,Institute of Microbial science and Technologies Ltd.
Aquatic Toxicology | Year: 2013
The net toxicity of different forms of mercury, in the long-term during their transformation processes, leads to the selection of resistant bacterial cells and this result in community tolerance which is pollution induced. Accordingly, based on profiles of a bacterial community structure, analysis of Hg resistant culturable bacteria and quantification of merA genes, we assessed development of pollution induced community tolerance in a mercury-polluted gradient in the Idrijca River. TTGE analysis did not show effects of mercury pollution to bacterial community diversity, while quantification of merA genes showed that merA genes can be correlated precisely (R2=0.83) with the total concentration of mercury in the biofilm microbial communities in the pollution gradient. © 2013 Elsevier B.V.