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Ding G.,Institute of Medicinal Plant Development | Ding G.,Chinese Institute of Materia Medica | Wang H.,Institute of Medicinal Plant Development | Li L.,Chinese Institute of Materia Medica | And 4 more authors.
Journal of Natural Products | Year: 2014

Trichodermone (1), the first spiro-cytochalasan with an unprecedented tetracyclic nucleus (7/5/6/5), together with its possible biosynthetic precursor aspochalasin D (2), was isolated from the endophytic fungus Trichoderma gamsii. Compound 2 displayed moderate inhibitory activity against HeLa cells with an IC50 value of 5.72 μM. © 2014 The American Chemical Society and American Society of Pharmacognosy.


PubMed | Institute of Medicinal Plant Development, Xinjiang Institute of Chinese and Ethnic Medicine, Jilin Agricultural University and Xiamen University
Type: Journal Article | Journal: Journal of Asian natural products research | Year: 2016

Five diarylpentanol derivatives including two new compounds stellerasme A (1), stellerasme B (2) were isolated from the aerial parts of Stelleropsis tianschanica. Their structures were elucidated by various spectroscopic techniques (UV, IR, MS, CD, 1D and 2D NMR). All compounds were evaluated for their cytotoxicity activity against HeLa and KB cell lines, and compound 1 showed selective activities against HeLa cell line with an IC50 value of 7.4M.


Liu H.,Institute of Medicinal Plant Development | Liu S.,University of Chinese Academy of Sciences | Liu S.,CAS Institute of Microbiology | Guo L.,CAS Institute of Microbiology | And 3 more authors.
Molecules | Year: 2012

Pestalafuranones A-E (compounds 1-5), five new 2(5H)-furanones, have been isolated from cultures of an isolate of Pestalotiopsis besseyi. The structures of these compounds were elucidated mainly by analysis of their NMR spectroscopic data and HRESIMS experiments. Pestalafuranones A-C (compounds 1-3) displayed weak inhibitory effects against HIV-1 replication in C8166 cells, whereas pestalafuranones D (4) and E (5) showed moderate antifungal activity against the plant pathogens Verticillium dahiae and Alternaria longipes. © 2012 by the authors.


You Y.,Tsinghua University | Zhu J.,Tsinghua University | Yang Z.,Institute of Medicinal Plant Development
Beijing Hangkong Hangtian Daxue Xuebao/Journal of Beijing University of Aeronautics and Astronautics | Year: 2012

A new envolving data stream clustering algorithm with fault-tolerance characteristic was proposed named FTGDStream (fault-tolerant grid-density clustering over data stream). It introduces appropriate relaxation of conditions for discover generalised knowledge in real world data polluted by noise. First, FTGDStream uses similarity measure technology and lifting wavelet to construct synopsis HLSFTS (hierarchical lifting scheme fault-tolerant synopses) to realize online micro-cluster phase. Second, FTGDStream uses grid-density clustering technology to realize offline macro-cluster phase. High compression ratio of HLSFTS in micro-cluster reduces the computation load of grid-density clustering algorithm in macro-cluster and improves the efficiency of two-layer algorithm. Simulation in UCI data set proves that FTGDStream is able to clustering any shape in data space and suitable for dealing with high-dimensional data streams. FTGDStream is an efficient clustering algorithm with fault-tolerance.


Meng X.,Institute of Medicinal Plant Development | Wang M.,Institute of Medicinal Plant Development | Wang X.,Changchun University of Chinese Medicine | Sun G.,Institute of Medicinal Plant Development | And 3 more authors.
Free Radical Research | Year: 2014

Notoginsenoside R1 (NGR1) is a novel phytoestrogen that is isolated from Panax notoginseng. We have recently found that NGR1 showed neuroprotection in vitro against oxidative stress through estrogen receptor (ER)-dependent activation of Akt/Nrf2 pathways. However, whether NGR1 has neuroprotective effect against cerebral ischemia-reperfusion (I/R) injury in vivo is unknown. In this study, we used in vivo and in vitro models of cerebral I/R injury that demonstrate middle cerebral artery occlusion and reperfusion in rats, as well as oxygen-glucose deprivation followed by reoxygenation (OGD/R) in primary cortical neurons. These models were used to evaluate NGR1 neuroprotection. Three-day pretreatment with NGR1 (20 mg/kg; i.p.) significantly improved neurologic outcomes and reduced cerebral infarct volume. Pretreatment of primary cortical neurons with NGR1 (25 μM) for 24 h prevented apoptosis and oxidative stress induced by OGD/R. NGR1 inhibited apoptosis by inhibiting mitochondrial membrane potential disruption, caspase-3 activation, and DNA fragmentation. NGR1 prevented oxidative stress by suppressing NADPH oxidase- and mitochondrion-derived superoxide and inhibiting production of malondialdehyde, protein carbonyl, and 8-hydroxydeoxyguanosine in vivo and in vitro. NGR1 induced ER-dependent activation of Akt/Nrf2 pathways by increasing ERα, ERβ, phospho-Akt, phospho-GSK3β, nuclear Nrf2, and HO-1 expression in vivo and in vitro. Pretreatment with ICI-182780, LY294002, or Snpp abolished NGR1-mediated neuroprotection against oxidative stress and apoptosis in vitro. In conclusion, NGR1 showed neuroprotection against cerebral I/R injury in vivo and in vitro. The mechanism of NGR1 neuroprotection involves inhibition of NADPH oxidase activity and mitochondrial dysfunction via ER-dependent activation of Akt/Nrf2 pathways. © 2014 Informa UK, Ltd.


Ma G.,Institute of Medicinal Plant Development | Yuan J.,National Engineering Laboratory of Southwest Endangered Medicinal Resource Development | Wu H.,Institute of Medicinal Plant Development | Cao L.,Institute of Medicinal Plant Development | And 9 more authors.
Journal of Natural Products | Year: 2013

Eight new cassane-type diterpenes, caesalpins A-H (1-8), were isolated from the ethyl acetate extract of Caesalpinia minax. Compound 1 displayed significant antiproliferative activity against HepG-2 (IC50 4.7 μM) and MCF-7 (IC50 2.1 μM) cells, and compounds 2 and 4 exhibited selective cytotoxic activities against MCF-7 (IC50 7.9 μM) and AGS (IC50 6.5 μM) cells. © 2013 The American Chemical Society and American Society of Pharmacognosy.


You Y.,Harbin Engineering University | Jianpei Z.,Harbin Engineering University | Yang Z.,Institute of Medicinal Plant Development
Proceedings - 2010 International Conference on Intelligent Computing and Cognitive Informatics, ICICCI 2010 | Year: 2010

Mining frequent patterns over streaming data has become an important research focus field with broad applications. However, the real-world data may be usually polluted by uncontrolled factors. Fault-tolerant frequent pattern can express more generalized information than frequent pattern which is absolutely matched. Therefore, a novel single-pass algorithm is proposed for efficiently mining top-k fault-tolerant frequent pattern from data streams without minimum support threshold specified by user. A novel data structure is developed for maintaining the essential information of itemsets generated so far. Experimental results show that the developed algorithm is an efficient method for mining top-k fault-tolerant frequent pattern from data streams. © 2010 IEEE.


Liu Y.,Institute of Medicinal Plant Development | Chen H.,Institute of Medicinal Plant Development | Yang Y.,Institute of Medicinal Plant Development | Zhang Z.,Institute of Medicinal Plant Development | And 11 more authors.
Molecules | Year: 2013

Agarwood is the fragrant resin-infused wood derived from the wounded trees of Aquilaria species. It is a valuable non-timber forest product used in fragrances and as medicine. Reforestation for Aquilaria trees in combination with artificial agarwood-inducing methods serves as a way to supply agarwood and conserve of wild Aquilaria stock. However, the existing agarwood-inducing methods produce poor-quality agarwood at low yield. Our study evaluated a novel technique for producing agarwood in cultivated Aquilaria trees, called the whole-tree agarwood-inducing technique (Agar-Wit). Ten different agarwood inducers were used for comparison of Agar-Wit with three existing agarwood-inducing methods. For Aquilaria trees treated with these ten inducers, agarwood formed and spread throughout the entire tree from the transfusion point in the trunk to the roots and branches of the whole tree. Agarwood yield per tree reached 2,444.83 to 5,860.74 g, which is 4 to 28 times higher than that by the existing agarwood-inducing methods. Furthermore, this agarwood derived from Agar-Wit induction was found to have a higher quality compared with the existing methods, and similar to that of wild agarwood. This indicates Agar-Wit may have commercial potential. Induction of cultivated agarwood using this method could satisfy the significant demand for agarwood, while conserving and protecting the remaining wild Aquilaria trees.


Mei X.-L.,Institute of Medicinal Plant Development | Zhao Z.,Institute of Medicinal Plant Development | Chen X.-D.,Institute of Medicinal Plant Development | Lan J.,Institute of Medicinal Plant Development
Zhongguo Zhongyao Zazhi | Year: 2013

To study the effect and mechanism of the light quality acting on Ganoderma lucidum, and provide a theoretical basis for G. lucidum mycelium cultivation, we focused on growth and endogenous IAA metabolism of G. lucidum mycelium under different light-emitting diode (LED) condition. The growth index, endogenous levels of IAA and Enzymes related to IAA metabolism and Polysaccharides content were investigated in different growth periods. Results showed that blue light irradiation was the best from the viewpoint of steady growth and polysaccharides accumulation, red light irradiation improved endogenous IAA level and promoted growth of mycelium in early stage of cultivation, green light irradiation decreased growth rate and fresh weight of mycelium, but increased drying rate. Enzymes related to IAA metabolism also significantly influenced by light quality. The activity of indole acetic acid oxidase (IAAO), peroxidase (POD) and tryptophan synthetase with blue light irradiation were showed high level in early time, but decreased later, and the IAA content was consistently at lower level than that in other treatments, while mycelium irradiated with yellow light showed the highest activity of both IAAO and tryptophan synthetase, and medium level of IAA content. In conclusion, the light quality affects growth and regulation of the level of endogenous IAA of G. lucidum mycelium.


Wang M.,Institute of Medicinal Plant Development | Lu S.,Institute of Medicinal Plant Development
Frontiers in Plant Science | Year: 2016

Reverse transcription-qPCR (RT-qPCR) has become a popular method for gene expression studies. Its results require data normalization by housekeeping genes. No single gene is proved to be stably expressed under all experimental conditions. Therefore, systematic evaluation of reference genes is necessary. With the aim to identify optimum reference genes for RT-qPCR analysis of gene expression in different tissues of Panax ginseng and the seedlings grown under heat stress, we investigated the expression stability of eight candidate reference genes, including elongation factor 1-beta (EF1-β), elongation factor 1-gamma (EF1-γ), eukaryotic translation initiation factor 3G1 (IF3G1), eukaryotic translation initiation factor 3B (IF3B), actin (ACT), actin11 (ACT11), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), and cyclophilin ABH-like protein (CYC), using four widely used computational programs: geNorm, Normfinder, BestKeeper, and the comparative ΔCt method. The results were then integrated using the web-based tool RefFinder. As a result, EF1-γ, IF3G1, and EF1-β were the three most stable genes in different tissues of P. ginseng, while IF3G1, ACT11, and GAPDH were the top three-ranked genes in seedlings treated with heat. Using three better reference genes alone or in combination as internal control, we examined the expression profiles of MAR, a multiple function-associated mRNA-like non-coding RNA (mlncRNA) in P. ginseng. Taken together, we recommended EF1-γ /IF3G1 and IF3G1/ACT11 as the suitable pair of reference genes for RT-qPCR analysis of gene expression in different tissues of P. ginseng and the seedlings grown under heat stress, respectively. The results serve as a foundation for future studies on P. ginseng functional genomics. © 2016 Wang and Lu.

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