Institute of Medical Technology

Zabrze, Poland

Institute of Medical Technology

Zabrze, Poland
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Desai K.K.,Institute of Medical Technology
International Journal of ChemTech Research | Year: 2014

The ligand 2′-Hydroxy-4′-butoxychalcone oxime (HBCO) was developed as a new analytical reagent for the gravimetric and spectrophotometric analysis of Ni(II) ion. In the pH range of 8.0 to 10.0 this reagent gives light green complex with Ni(II). Job's method of continuous variation and Yoe and Jone's mole ratio method revealed the stoichiometry of the complex to be 1:2 [M:L]. The obeyence of Beer's law was studied and the molar absorptivity and Sandell's sensitivity were calculated. The reagent and its complex have been characterized by elemental analysis and IR spectra. The reagent has been used for the determination of Nickel content in German silver alloy.


Patel N.P.,Shree Jayendrapuri Arts and Science College | Desai K.K.,Institute of Medical Technology
Oriental Journal of Chemistry | Year: 2011

Numerous heterocyclic azo dyes have been reported for the spectrophotometric determination of Cu(II). Here, we report a new heterocyclic azo dye 1-( 2'Chloro-4'-sulphophenyl-S-methyl-azo-(2''carboxy-5"-sulphonic acid)-5-pyrazolone [CSMACSP] for the spectrophotometric determination of Cu(II).The method Is simple, rapid and sensitive. Light green colour 1:1 (M:L) complex formed between Cu(I I) and CSMACSP in the pH rage 2.0-5.0. The absorption spectrum shows as shoulder at 490 nm. The molar absorptivity and Sandell's sensitivity is found to be 0.683×103 lit.mol- 1 cm1 and 0.093 μg/ cm2, respectively. A number of foreign ions are tested for their interference. Elemental analysis and IR spectra of the reagent and its complex were reported. The reagent was successfully applied for the determination of Cu(II) content in brass alloy.


PubMed | b Health and Medical Technology College, Institute of Medical Technology and c Al Nahrain Medical College
Type: Journal Article | Journal: Renal failure | Year: 2016

Oxidative stress has been linked to disease progression, including chronic renal failure (CRF). The aim of the present study was to determine malondialdehyde (MDA) as a sign of lipid peroxidation, and to investigate the association between antioxidant activities and three trace elements, in 49 patients with CRF. The erythrocyte and plasma trace elements [selenium (Se), zinc (Zn), and copper (Cu)] and antioxidant defense levels were determined: glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), vitamins E and C. The obtained values were compared with 42 age- and sex-matched healthy controls. There were significantly lower mean values of plasma Se, GPx, vitamins E and C, erythrocyte Se, SOD and CAT levels in the patient group compared to the control group (p < 0.001). Plasma MDA showed a significant increase in all CRF patients in comparison with controls. No significant difference was found in plasma Cu, Zn, and erythrocyte GPx, Cu and Zn levels between patient and control groups. These findings indicate oxidative stress is present in patients of CRF, and may serve to establish a simple protocol for evaluation of renal function.


Patel M.,Institute of Medical Technology | Ramavataram D.V.S.S.,Surat Municipal Institute of Medical Education and Research
Indian Journal of Clinical Biochemistry | Year: 2012

Iron is an essential trace element and plays a number of vital roles in biological system. It also leads the chains of pathological actions if present in excess and/or present in free form. Major portion of iron in circulation is associated with transferrin, a classical iron transporter, which prevent the existence of free iron. The fraction of iron which is free of transferrin is known as non transferrin bound iron. Along with the incidence in iron over loaded patient non transferrin bound iron has been indicated in patients without iron overload. It has been suggested as cause as well as consequence in a number of pathological conditions. The major organs influenced by iron toxicity are heart, pancreas, kidney, organs involved in hematopoiesis etc. The most commonly suggested way for iron mediated pathogenesis is through increased oxidative stress and their secondary effects. Generation of free oxygen radicals by iron has been well documented in Fenton chemistry and Haber-Weiss reaction. Non transferrin bound iron has obvious chance to generate the free reactive radicals as it is not been shielded by the protective carrier protein apo transferrin. The nature of non transferrin bound iron is not clear at present time but it is definitely a group of heterogenous iron forms free from transferrin and ferritin. A variety of analytical approaches like colorimetry, chromatography, fluorimetry etc. have been experimented in different research laboratories for estimation of non transferrin bound iron. However the universally accepted gold standard method which can be operated in pathological laboratories is still to be developed. © 2012 Association of Clinical Biochemists of India.


Kotas M.,Silesian University of Technology | Jezewski J.,Institute of Medical Technology | Matonia A.,Institute of Medical Technology | Kupka T.,Institute of Medical Technology
Computer Methods and Programs in Biomedicine | Year: 2010

The noninvasive fetal electrocardiography is a source of more precise information on the fetal heart activity than the measurements based on Doppler ultrasound signals. However, the clinical diagnostic applications of this technique are limited by difficulty with successful detection of small amplitude fetal QRS complexes. In this study, we investigate the influence of different stages of fetal signals processing on the detection performance. The main propositions of the paper are: application of normalized matched filtering to fetal QRS complexes enhancement and a new approach to the final detection of the complexes. Compared to the classical detectors, the proposed new one allows a significant increase of the detection performance for signals of very different quality. © 2009 Elsevier Ireland Ltd. All rights reserved.


Aziz M.A.,Institute of Medical Technology | Majeed G.H.,Institute of Medical Technology | Diab K.S.,Health and Medical Technology College | Al-Tamimi R.J.,Al Nahrain Medical College
Renal Failure | Year: 2016

Oxidative stress has been linked to disease progression, including chronic renal failure (CRF). The aim of the present study was to determine malondialdehyde (MDA) as a sign of lipid peroxidation, and to investigate the association between antioxidant activities and three trace elements, in 49 patients with CRF. The erythrocyte and plasma trace elements [selenium (Se), zinc (Zn), and copper (Cu)] and antioxidant defense levels were determined: glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), vitamins E and C. The obtained values were compared with 42 age- and sex-matched healthy controls. There were significantly lower mean values of plasma Se, GPx, vitamins E and C, erythrocyte Se, SOD and CAT levels in the patient group compared to the control group (p < 0.001). Plasma MDA showed a significant increase in all CRF patients in comparison with controls. No significant difference was found in plasma Cu, Zn, and erythrocyte GPx, Cu and Zn levels between patient and control groups. These findings indicate oxidative stress is present in patients of CRF, and may serve to establish a simple protocol for evaluation of renal function. © 2015 Taylor & Francis.


Jebur M.S.,Institute of Medical Technology
African Journal of Microbiology Research | Year: 2011

Yeast infections of the vagina are more prevalence and frequently recurring problem of women. Such infections are caused primarily by Candida albicans and apparently occur when the environmental balance in the-vagina is disturbed. Lactobacillus acidopbilus restricts the growth of pathogenic microorganisms per se displayingwide inhibitory spectrum substances. Fifteen (30%) C. albicans isolates were obtained through 50 vaginal swabs from patients who visited the obstetrics and Gynecology clinic departments of hospitals in Baghdad city during the period of March to July 2010. Local isolates of L. acidophilus was evaluated as antifungal activity against vaginal isolates of C. albicans. Such activity was comprised with common four antifungal agents (Nystatine, Fluconazol, Griseofulvin and Amphotricin B). Susceptibility of C. albicans isolates to L. acidophilus bacteria were determined by means of disc diffusion assay. Results of susceptibility were showed in-vitro that all isolates 15 (100%) sensitive to Nystatine after cultivation on ESDA agar at 37°C for 24 h. Sensitivity of C. albicans to L. acidophilus suspension was less than Nystaine but still high with 13 sensitive isolates (86.6%). Fluconazol and Griseofulvin antifungal agents were showed same susceptibility of 10 sensitive isolates (66.6%) against C. albicans growth, while Amphotricin B had relatively low sensitivity with 7 isolates (46.5%). All results of susceptibility are being detected by the importance of L. acidophilus bacteria in re equilibrium normal flora of vagina that prevent or reduce the chances of vaginal infection especially recurrent vaginal candidiasis (RVC) cases. Also the findings of the study suggest that using of yoghurt as local application can be an important role to reduce or prevent RVC cases, and this can be verify by continuous efforts to make sure of probiotic effects of lactobacilli species. © 2011 Academic Journals.


Castro A.R.,Centers for Disease Control and Prevention | Castro A.R.,Institute of Medical Technology | Mody H.C.,Institute of Medical Technology | Parab S.Y.,Span Diagnostics Ltd. | And 4 more authors.
Sexually Transmitted Infections | Year: 2010

Objective: The development of a rapid immunofiltration (flow-through) test for the simultaneous detection of non-treponemal and treponemal antibodies in the serum of patients with syphilis. Methods: The assay is rapid, inexpensive, and requires limited expertise in interpreting the results. The test is based on the principle of immunofiltration, with two antigens and control material spotted on the membrane of a through-flow device. A positive test is characterised by the appearance of three red/magenta spots within 2-10 min. Results: A total of 376 banked serum samples obtained from the Georgia Public Health Laboratory was examined by the flow-through test, the rapid plasma reagin (RPR) test and the Treponema pallidum passive particle agglutination assay (TPPA). The sensitivity and specificity of the non-treponemal spot were 96.5% and 97.7%, respectively, when compared with the RPR test, and the sensitivity and specificity of the treponemal test spot were 97.3% and 99.1% when compared with the TPPA test. In addition, the test yielded equivalent results to those obtained in comparator tests when 104 sera from cases of syphilis of known stage, 49 sera from diseases other than syphilis and 23 sera known to exhibit biological false-positive reactions were tested in parallel. Conclusions: These results indicate that the dual treponemal and non-treponemal assay could be used as a screen and confirmatory test for the serological diagnosis of syphilis in remote or resource-poor settings where there is a need to provide counselling and treatment at the initial consultation.


PubMed | Institute of Medical Technology
Type: Journal Article | Journal: North American journal of medical sciences | Year: 2012

Probiotics are live microorganisms which are mainly strains of Lactobacillus spp., Bifidobacterium spp. When administered in adequate amounts, these microorganisms offer a health benefit for the host. Probiotic organisms are also available commercially in milk, sour milk, ice cream and other foods.To identify bacterial species isolated from burn wounds, and also to evaluate (In-vitro) the therapeutic efficacy of Lacto. acidophilus against these bacterial isolates. To compare this activity to other antibacterial agents which are used medically in the treatment of burn wound cases.Burn wound swabs were obtained from 50 patients who had been admitted to hospitals in Baghdad during August to November 2009. These swabs were inoculated onto enriched and differential culture media. Subcultures were performed on selective media. The necessary biochemical tests were conducted and the organisms identified using standard procedures. Susceptibility of isolated pathogens to local isolates Lacto. Acidophilus (with 110(8) cells/mL) and 10 commonly used burn wounds antibiotics was examined using standard susceptibility testing.Ninety different organisms were isolated. Gram-positive cocci accounted for 16 (17.7%) and gram-negative bacilli for 74 (82.2%) bacterial isolates. Pseudomonas aeruginosa 30(33.3%) were the most commonly isolated organisms, followed by Escherichia coli, Enterobacter spp., Klebsiella spp., Proteus spp.(22.2,20,4.4,2.2%), respectively. Staphylococcus aureus isolates were performed in 8(8.8%). However, the incidence of Staphylococcus epidermidis was 2 (2.2%), while -haemolytic Streptococci was 4(4.4%). In susceptibility testing, Lacto. acidophilus had coverage against 90 (100%) of 74 gram-negative and 16 of gram-positive bacteria tested. The coverage of the remaining 10 antibacterial agents used was different in their activity (resistance or sensitivity), which ranged between 50-100%.The results of the study concluded that lactobacillus acidophilus concentration of 110(8) cells/mL had a high activity to inhibit the growth in-vitro of all pathogenic gram-positive and gram-negative bacteria, which cause burn wound infections. This indicated the therapeutic efficacy of lactobacillus acidophilus bacteria.


PubMed | Institute of Medical Technology
Type: Journal Article | Journal: Indian journal of clinical biochemistry : IJCB | Year: 2013

Iron is an essential trace element and plays a number of vital roles in biological system. It also leads the chains of pathological actions if present in excess and/or present in free form. Major portion of iron in circulation is associated with transferrin, a classical iron transporter, which prevent the existence of free iron. The fraction of iron which is free of transferrin is known as non transferrin bound iron. Along with the incidence in iron over loaded patient non transferrin bound iron has been indicated in patients without iron overload. It has been suggested as cause as well as consequence in a number of pathological conditions. The major organs influenced by iron toxicity are heart, pancreas, kidney, organs involved in hematopoiesis etc. The most commonly suggested way for iron mediated pathogenesis is through increased oxidative stress and their secondary effects. Generation of free oxygen radicals by iron has been well documented in Fenton chemistry and Haber-Weiss reaction. Non transferrin bound iron has obvious chance to generate the free reactive radicals as it is not been shielded by the protective carrier protein apo transferrin. The nature of non transferrin bound iron is not clear at present time but it is definitely a group of heterogenous iron forms free from transferrin and ferritin. A variety of analytical approaches like colorimetry, chromatography, fluorimetry etc. have been experimented in different research laboratories for estimation of non transferrin bound iron. However the universally accepted gold standard method which can be operated in pathological laboratories is still to be developed.

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