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Jebur M.S.,Institute of Medical Technology
African Journal of Microbiology Research | Year: 2011

Yeast infections of the vagina are more prevalence and frequently recurring problem of women. Such infections are caused primarily by Candida albicans and apparently occur when the environmental balance in the-vagina is disturbed. Lactobacillus acidopbilus restricts the growth of pathogenic microorganisms per se displayingwide inhibitory spectrum substances. Fifteen (30%) C. albicans isolates were obtained through 50 vaginal swabs from patients who visited the obstetrics and Gynecology clinic departments of hospitals in Baghdad city during the period of March to July 2010. Local isolates of L. acidophilus was evaluated as antifungal activity against vaginal isolates of C. albicans. Such activity was comprised with common four antifungal agents (Nystatine, Fluconazol, Griseofulvin and Amphotricin B). Susceptibility of C. albicans isolates to L. acidophilus bacteria were determined by means of disc diffusion assay. Results of susceptibility were showed in-vitro that all isolates 15 (100%) sensitive to Nystatine after cultivation on ESDA agar at 37°C for 24 h. Sensitivity of C. albicans to L. acidophilus suspension was less than Nystaine but still high with 13 sensitive isolates (86.6%). Fluconazol and Griseofulvin antifungal agents were showed same susceptibility of 10 sensitive isolates (66.6%) against C. albicans growth, while Amphotricin B had relatively low sensitivity with 7 isolates (46.5%). All results of susceptibility are being detected by the importance of L. acidophilus bacteria in re equilibrium normal flora of vagina that prevent or reduce the chances of vaginal infection especially recurrent vaginal candidiasis (RVC) cases. Also the findings of the study suggest that using of yoghurt as local application can be an important role to reduce or prevent RVC cases, and this can be verify by continuous efforts to make sure of probiotic effects of lactobacilli species. © 2011 Academic Journals. Source


Patel M.,Institute of Medical Technology | Ramavataram D.V.S.S.,Surat Municipal Institute of Medical Education and Research
Indian Journal of Clinical Biochemistry | Year: 2012

Iron is an essential trace element and plays a number of vital roles in biological system. It also leads the chains of pathological actions if present in excess and/or present in free form. Major portion of iron in circulation is associated with transferrin, a classical iron transporter, which prevent the existence of free iron. The fraction of iron which is free of transferrin is known as non transferrin bound iron. Along with the incidence in iron over loaded patient non transferrin bound iron has been indicated in patients without iron overload. It has been suggested as cause as well as consequence in a number of pathological conditions. The major organs influenced by iron toxicity are heart, pancreas, kidney, organs involved in hematopoiesis etc. The most commonly suggested way for iron mediated pathogenesis is through increased oxidative stress and their secondary effects. Generation of free oxygen radicals by iron has been well documented in Fenton chemistry and Haber-Weiss reaction. Non transferrin bound iron has obvious chance to generate the free reactive radicals as it is not been shielded by the protective carrier protein apo transferrin. The nature of non transferrin bound iron is not clear at present time but it is definitely a group of heterogenous iron forms free from transferrin and ferritin. A variety of analytical approaches like colorimetry, chromatography, fluorimetry etc. have been experimented in different research laboratories for estimation of non transferrin bound iron. However the universally accepted gold standard method which can be operated in pathological laboratories is still to be developed. © 2012 Association of Clinical Biochemists of India. Source


Desai K.K.,Institute of Medical Technology
International Journal of ChemTech Research | Year: 2014

The ligand 2′-Hydroxy-4′-butoxychalcone oxime (HBCO) was developed as a new analytical reagent for the gravimetric and spectrophotometric analysis of Ni(II) ion. In the pH range of 8.0 to 10.0 this reagent gives light green complex with Ni(II). Job's method of continuous variation and Yoe and Jone's mole ratio method revealed the stoichiometry of the complex to be 1:2 [M:L]. The obeyence of Beer's law was studied and the molar absorptivity and Sandell's sensitivity were calculated. The reagent and its complex have been characterized by elemental analysis and IR spectra. The reagent has been used for the determination of Nickel content in German silver alloy. Source


Patel N.P.,Shree Jayendrapuri Arts and Science College | Desai K.K.,Institute of Medical Technology
Oriental Journal of Chemistry | Year: 2011

Numerous heterocyclic azo dyes have been reported for the spectrophotometric determination of Cu(II). Here, we report a new heterocyclic azo dye 1-( 2'Chloro-4'-sulphophenyl-S-methyl-azo-(2''carboxy-5"-sulphonic acid)-5-pyrazolone [CSMACSP] for the spectrophotometric determination of Cu(II).The method Is simple, rapid and sensitive. Light green colour 1:1 (M:L) complex formed between Cu(I I) and CSMACSP in the pH rage 2.0-5.0. The absorption spectrum shows as shoulder at 490 nm. The molar absorptivity and Sandell's sensitivity is found to be 0.683×103 lit.mol- 1 cm1 and 0.093 μg/ cm2, respectively. A number of foreign ions are tested for their interference. Elemental analysis and IR spectra of the reagent and its complex were reported. The reagent was successfully applied for the determination of Cu(II) content in brass alloy. Source


Patel N.B.,Shree Jayendrapuri Arts and Science College | Desai K.K.,Institute of Medical Technology
International Journal of ChemTech Research | Year: 2012

The ligand 2,4-dihydroxy-5-bromobutyrophenone oxime [DHBBO] was developed as a new analytical reagent for the gravimetric and spectrophotometric analysis of Ni(II) ion. In the pH range of 7.0 to 10.0 this reagent gives light green precipitate with Ni(II). Job's method of continuous variation and Yoe and Jones mole ratio method revealed the stoichiometry of the complex to be 1:2. The obeyence of Beer's law was studied and the molar absorptivity and Sandell's sensitivity was calculated. The reagent and its complex have been characterized by elemental analysis and IR spectra. The complex was subjected to thermogravimetric analysis to study its decomposition pattern. The reagent has been used for the analysis of german silver. Source

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