Institute of Medical Climatology and Rehabilitative Treatment

Vladivostok, Russia

Institute of Medical Climatology and Rehabilitative Treatment

Vladivostok, Russia

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Golokhvast K.,Far Eastern Federal University | Vitkina T.,Institute of Medical Climatology and Rehabilitative Treatment | Gvozdenko T.,Institute of Medical Climatology and Rehabilitative Treatment | Yankova V.,Institute of Medical Climatology and Rehabilitative Treatment | And 8 more authors.
Oxidative Medicine and Cellular Longevity | Year: 2015

Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with different levels of microparticle air pollution.We analyzed the distribution of microparticles in snow samples by size and content. ELISA and flow cytometrymethods were employed to determine the parameters of the thiol-disulfide metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800 μm or more were predominant (96%), while in the industrial areas, they tended to be less than 50 μm (93%), including size 200-300nm (7%). In the industrial areas, we determined the oxidative modification of proteins (21% compared to the park areas, p = 0.05) andDNA (12%, p ≤ 0.05), as well as changes in leukocytes' energy potential (53%, p ≤ 0.05). An increase in total antioxidant activity (82%, p ≤ 0.01) and thiol-disulfide system response (thioredoxin increasing by 33%, p ≤ 0.01; glutathione, 30%, p ≤ 0.01 with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures fromsignificant oxidative damage. Copyright © 2015 Kirill Golokhvast et al.


PubMed | Institute of Medical Climatology and Rehabilitative Treatment, University of Crete, Russian Academy of Sciences and Far Eastern Federal University
Type: | Journal: Oxidative medicine and cellular longevity | Year: 2015

Atmospheric microsized particles producing reactive oxygen species can pose a serious health risk for city residents. We studied the responses of organisms to microparticles in 255 healthy volunteers living in areas with different levels of microparticle air pollution. We analyzed the distribution of microparticles in snow samples by size and content. ELISA and flow cytometry methods were employed to determine the parameters of the thiol-disulfide metabolism, peroxidation and antioxidant, genotoxicity, and energy state of the leukocytes. We found that, in the park areas, microparticles with a size of 800m or more were predominant (96%), while in the industrial areas, they tended to be less than 50m (93%), including size 200-300nm (7%). In the industrial areas, we determined the oxidative modification of proteins (21% compared to the park areas, p 0.05) and DNA (12%, p 0.05), as well as changes in leukocytes energy potential (53%, p 0.05). An increase in total antioxidant activity (82%, p 0.01) and thiol-disulfide system response (thioredoxin increasing by 33%, p 0.01; glutathione, 30%, p 0.01 with stable reductases levels) maintains a balance of peroxidation-antioxidant processes, protecting cellular and subcellular structures from significant oxidative damage.


Novgorodtseva T.P.,Institute of Medical Climatology and Rehabilitative Treatment | Kantur T.A.,Institute of Medical Climatology and Rehabilitative Treatment | Karaman Y.K.,Institute of Medical Climatology and Rehabilitative Treatment | Antonyuk M.V.,Institute of Medical Climatology and Rehabilitative Treatment | Zhukova N.V.,RAS A.V. Zhirmunsky Institute of Marine Biology
Lipids in Health and Disease | Year: 2011

Background: Modification of fatty acids (FA) composition in erythrocytes lipids as an early indicator of the development of arterial hypertension (AH) and lipid disorders. Methods. We included 34 patients with arterial hypertension and 11 healthy individuals. Each patient was examined the lipid composition of serum. From erythrocytes by gas chromatography were identified fatty acids. The quantitative composition of the erythrocyte lipids FA in patients with AH presented with saturated and polyunsaturated monoenic acids with carbon chain lengths from C12 to C22. Results: In all hypertensive patients is disturbed lipid FA composition of erythrocytes. The patients with a normal level of serum lipids revealed deficiency of polyunsaturated acids n6-linoleic (18:2 n6), arachidonic (20:4 n6), dokozatetraenic 14,4% (22:4 n6). The patients with dislipidemia installed more profound restructuring of the lipid matrix of the membrane of erythrocytes. A decrease in polyunsaturated fatty acids family n3: dokozapentaenovoy (22:5 n3), docosahexaenoic PUFA (22:6 n3), the total value of n3 PUFA in 1,3 times was revealed. Conclusion: Thus, modification of erythrocyte FA are fairly subtle indicator of pathology of lipid metabolism, which manifest themselves much earlier than changes in the lipoprotein of blood plasma. © 2011 Novgorodtseva et al; licensee BioMed Central Ltd.


Novgorodtseva T.P.,Institute of Medical Climatology and Rehabilitative Treatment | Karaman Y.K.,Institute of Medical Climatology and Rehabilitative Treatment | Zhukova N.V.,RAS A.V. Zhirmunsky Institute of Marine Biology | Lobanova E.G.,Institute of Medical Climatology and Rehabilitative Treatment | And 2 more authors.
Lipids in Health and Disease | Year: 2011

Background: Disturbances of the fatty acids composition in plasma and red blood cells and eicosanoid synthesis play an important role in the metabolic syndrome (MS) formation. Methods. The observation group included 61 people with metabolic syndrome (30 patients with MS and normal levels of insulin, 31 people with MS and insulin resistance - IR). The parameters of carbohydrate and lipid metabolism in blood serum were examined. The composition of nonesterified fatty acids (NEFA), fatty acid (FA) of red blood cells lipids was analyzed by gas-liquid chromatography. Eicosanoids level in MS patients blood serum was studied by enzyme immunoassay. Results: In MS patients in the absence of glucose-insulin homeostasis disturbances and in patients with IR the accumulation of polyunsaturated fatty acids (18:2 n6, 18:3 n3, 22:4 n6) and lower pool of saturated FA (12:0, 14:0, 16: 0, 17:0) in plasma were discovered. A deficit of polyunsaturated FA (18:3 n3, 20:4 n6) with a predominance of on-saturated FA (14:0, 18:0) in erythrocyte membranes was revealed. In MS patients regardless of the carbohydrate metabolism status high levels of leukotriene B4 and 6-keto-prostaglandin-F1 in serum were found. The development of IR in MS patients leads to increased synthesis of thromboxane A2. Conclusion: The results revealed a disturbance in nonesterified fatty acids of plasma lipids and red blood cells, eicosanoid synthesis in MS patients. The breach of the plasma and cell membranes fatty acids compositions, synthesis of vasoactive and proinflammatory eicosanoids is an important pathogenetic part of the MS development. © 2011 Novgorodtseva et al; licensee BioMed Central Ltd.


Karaman Y.K.,Institute of Medical Climatology and Rehabilitative Treatment | Novgorodtseva T.P.,Institute of Medical Climatology and Rehabilitative Treatment | Gvozdenko T.A.,Institute of Medical Climatology and Rehabilitative Treatment | Bivalkevich N.V.,Institute of Medical Climatology and Rehabilitative Treatment
Bulletin of Experimental Biology and Medicine | Year: 2012

The new model of nonalcoholic steatohepatitis in rats is based on alimentary effects of hypercaloric diet including much cholesterol and beef fat. This model reproduces structural and functional disorders in the liver, including hepatocyte fatty degeneration, parenchymatous necrosis and fi brosis paralleled by dyslipidemia and is intended for studies of the mechanisms of formation, progress, and therapy of liver diseases of noninfectious origin. © 2012 Springer Science+Business Media, Inc.


Zhukova N.V.,Far Eastern Federal University | Novgorodtseva T.P.,Institute of Medical Climatology and Rehabilitative Treatment | Denisenko Y.K.,Institute of Medical Climatology and Rehabilitative Treatment
Lipids in Health and Disease | Year: 2014

Background: Contradictory data on consequences of prolonged high-fat diet requires a detailed study of the influence of nutritional high-fat load mechanisms on the peculiarity of lipid metabolism in blood and liver. The present study was undertaken to investigate the fatty acid composition of polar and neutral lipids of the blood plasma, erythrocytes and liver in Wistar rats under the conditions of a prolonged high-fat diet. Methods. The study was conducted on 60 adult white male Wistar rats. The animals were fed on a high-fat diet consisted of the beef fat and cholesterol (19% and 2% of the total diet, respectively) up to 180 days. The fatty acid composition of the polar and neutral lipids of plasma, erythrocytes and liver were analyzed by the gas chromatography. Statistical data processing was performed by the methods of descriptive statistics with Statistica 6.0. Results: The prolonged unbalanced diet rich in cholesterol and saturated fatty acids resulted in compensatory biosynthesis of the fatty acids in the rat's liver, the inhibition of synthesis of apoproteins and lipoproteins, disruption of the active transport of fatty acids to tissue cells. This launched the accumulation of 20:4n-6, 20:5n-3, 22:5n-3, and 22:6n-3 in the liver and blood plasma and deficiency of 18:2n-6, 20:5n-3 and 22:6n-3 in the erythrocytes. Conclusions: Adaptive adjustment of lipid metabolism un0064er conditions of the high-fat diet induced inhibition of the formation of lipoproteins (VLDL cholesterol) in the liver, compensatory synthesis of 18:1n-9, 20:5n-3, and 20:3n-6 with primary esterification of PUFA n-3 series to neutral lipids. © 2014 Zhukova et al.; licensee BioMed Central Ltd.


Novgorodtseva T.P.,Institute of Medical Climatology and Rehabilitative Treatment | Denisenko Y.K.,Institute of Medical Climatology and Rehabilitative Treatment | Zhukova N.V.,Far Eastern Federal University | Antonyuk M.V.,Institute of Medical Climatology and Rehabilitative Treatment | And 2 more authors.
Lipids in Health and Disease | Year: 2013

Background: Early preclinical diagnosis of COPD is urgent. We proposed that fatty acid composition of red blood cells may serve as a prognostic test for the complications in the chronic respiratory diseases. Fatty acid composition of the erythrocyte membranes in patients with chronic respiratory diseases (chronic bronchitis, CB, and stable chronic obstructive pulmonary disease, COPD) was studied. It was established that modification of the fatty acid composition in the erythrocyte membranes was unidirectional in both groups of patients. Methods. Patients with CB and stable COPD (group A, GOLD 1) (15 subjects in each group) were studied in clinic. The activity of the inflammatory process was evaluated by the phagocytic activity of neutrophils, cytokine levels and cytokine receptors in the blood serum (TNF, sTNF-RI, bFGF, TGF-β, IL-8). Fatty acid (FA) composition of the erythrocyte membranes was analyzed by gas liquid chromatography. Statistical data processing was performed by the methods of descriptive statistics with Statistica 6.0. Results: In both groups (CB and COPD), a significant accumulation of the saturated FAs (14:0, 15:0, 18:0) was established. The amount of the arachidonic acid (20:4n-6) was increased by 13% (< 0.05) in CB patients and by 41% (< 0.001) in COPD patients, as compared with healthy persons. The elevated level of the PUFA n-6 in the erythrocytes membranes in patients with chronic respiratory diseases confirms that proinflammatory (leukotriene B4) and bronchospasm (prostaglandin D2) mediator substrates is increased. The level of the eicosapentaenoic acid (20:5n-3) was decreased by 32% (< 0.05) in CB patients and 2-fold (< 0.001) in COPD patients. The observed increase in the 20:4n-6/20:5n-3 ratio - 1.5-fold (< 0.001) in CB patients and 3-fold in COPD patients - can be a specific marker of the adverse course of the respiratory pathology and the chronic inflammatory development. Conclusions: Chronic respiratory disease development is associated with the disturbance of the fatty acid composition in erythrocyte membranes and disbalance of the ratio between precursor of pro- and antiinflammatory eicosanoids. © 2013 Novgorodtseva et al.; licensee BioMed Central Ltd.

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