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Frank G.A.,Pa Herzen Moscow Oncology Research Institute | Andreyeva Yu.Yu.,Pa Herzen Moscow Oncology Research Institute | Gorelik M.Z.,Primorye Territorial Morbid Anatomy Bureau | Zavalishina L.E.,Pa Herzen Moscow Oncology Research Institute | And 4 more authors.
Arkhiv Patologii

The paper analyzes 10 years' experience in HER2 status testing in breast cancer in Russia. The ASCO/CAP HER2 testing guidelines adaptable to the work of pathologists in Russia are considered. Source

Zasadkevich Yu.M.,Ural Federal University | Brilliant A.A.,Institute of Medical Cell Technologies | Sazonov S.V.,Ural Federal University
Arkhiv Patologii

The review gives data on the structure of cadherin cell adhesion molecules, their role in the body's development and malignant tumor progression. It describes cadherins that are considered to play the most important role in the development of a tumor process: E-, P-, and N-cadherins that belong to type I classical cadhedrins and VE-cadhedrin that does to type II cadherins. Particular emphasis is placed on the signal mechanisms with involvement of cadherins and cadherin-related molecules, which are realized in the body in health and in tumor transformation of cells. Source

Tsaur G.A.,Ural Federal University | Riger T.O.,Institute of Medical Cell Technologies | Popov A.M.,Institute of Medical Cell Technologies | Nasedkina T.V.,Russian Academy of Sciences | And 6 more authors.
Klinichescheskaya Laboratornaya Diagnostika

The occurrence of minimal residual disease is an important prognostic/actor under acute lymphoblastic leucosis in children and adults. In overwhelming majority of research studies bone marrow is used to detect minimal residual disease. The comparative characteristic of detection of minimal residual disease in peripheral blood and bone marrow was carried out. The prognostic role of occurrence of minimal residual disease in peripheral blood and bone marrow tinder therapy according protocol MLL-Baby was evaluated The analysis embraced 142 pair samples from 53 patients with acute lymphoblastic leucosis and various displacements of gene MLL younger than 365 days. The minimal residual disease was detected by force of identification of chimeric transcripts using polymerase chain reaction in real-time mode in 7 sequential points of observation established by protocol of therapy. The comparability of results of qualitative detection of minimal residual disease in bone marrow and peripheral blood amounted to 84.5%. At that, in all 22 (15.5%) discordant samples minimal residual disease was detected only in bone marrow. Despite of high level of comparability of results of detection of minimal residual disease in peripheral blood and bone marrow the occurrence of minimal residual disease in peripheral blood at various stages of therapy demonstrated no independent prognostic significance. The established differences had no relationship with sensitivity of method determined by value of absolute expression of gene ABL. Most likely, these differences reflected real distribution of tumor cells. The results of study demonstrated that application of peripheral blood instead of bone marrow for monitoring of minimal residual disease under acute lymphoblastic leucosis in children of first year of life is inappropriate. At the same time, retention of minimal residual disease in TH4 in bone marrow was an independent and prognostic unfavorable factor under therapy of acute lymphoblastic leucosis of children offirstyear of life according protocol MLL-Baby (OO=7.326, confidence interval 2.378-22.565). Source

Drui A.E.,Regional Childrens Hospital No. 1 | Drui A.E.,Ural Federal University | Shorikov E.V.,Regional Childrens Hospital No. 1 | Shorikov E.V.,Institute of Medical Cell Technologies | And 10 more authors.
Voprosy Onkologii

Deletion of CDKN2A/pl6 gene located in the 9p21.3 region is common alteration for many types of cancer. It was described in neuroblastoma patients as well, while its prognostic significance is contradictory. The aim was to determine the epidemiology and prognostic impact of 9p deletion in neuroblastoma patients. 9p status was analyzed by MLPA in 100 tumor samples from primary neuroblastoma patients. Prognostic significance was estimated by overall (OS) and event-free survival (EFS) with median of follow-up time 28 months (range 1-166 months). 9p deletion was revealed in 8 (8.0%) patients, in 5 of them localized in 9p21-23 loci and in 3 involved only locus 9p21.3 (CDKN2A/pl6). Stage distribution was as follow: stage 1-2 patients, II-1, III-2, IV-3 and no patients with IVS stage. We didn't find any association of 9p deletion with clinical (stage, histological subtypes, age) or genetic (MYCN amplification (MNA), lp, llq deletions) risk factors. Presence of 9p deletion resulted in dramatic decreasing of survival rates: both EFS and OS were 0.00 vs. 0.60±0.06 and 0.68±0.06 correspondingly, p=0.035, p=0.014). Prognostic significance of this aberration retained in patients with localized neuroblastoma (EFS and OS 0.00 vs. 0.85±0.06 and 0.91±0.05 respectively, p=0.058, p=0.031) as well as in MYCN non-amplified patients (EFS and OS 0.00 vs. 0.68±0.06 and 0.75±0.06, p=0.047, p=0.017). In multivariate analysis of OS performed by stage, age, MNA, lp, 9p deletions and 17q gain as covariates patients with stage IV (p=0.042), MNA (p=0.049) and 9p deletion (p=0.041) had significantly poor survival. Thus 9p deletion involving CDKN2A/pl6 gene demonstrated strongly negative prognostic impact. Significance of this abnormality retains in favorable groups of patients with localized rumor and without MYCN amplification. Source

Popov A.M.,Ural Federal University | Shorikov E.V.,Institute of Medical Cell Technologies | Verzhbitskaya T.Yu.,Institute of Medical Cell Technologies | Tsaur G.A.,Institute of Medical Cell Technologies | And 4 more authors.
Voprosy Onkologii

The purpose of the study was to evaluate the prognostic value of the detection of tumor cells in the bone marrow (BM) in children with neuroblastoma (NB) by flow cytometry. The detection of tumor cells was performed in BM of 51 patients with NB (24 boys and 27 girls) aged from 6 days to 15 years (median - 1 year 3 months). Flow cytometry allowed determining NB cells in BM in a much larger number of cases than cytomorphology (49.0 % and 29.4 % of patients, respectively). Patients, in whom NB cells were not detected in BM by flow cytometry, had significantly better event-free and overall survival rates as well as progression free survival (83, 5 %, 87, 7 % and 86, 8 %, respectively) compared with those in whom immunophenotyping revealed the tumor cells (28, 0 %, 35, 87 % and 34, 3 %, respectively). The prognostic value of the detection of BM lesion by flow cytometry was also confirmed in selected groups of patients with other criteria of stratification. Therefore the detection of tumor cells in BM by flow cytometry could potentially be considered in conjunction with other factors in choosing treatment strategy in patients with NB. Source

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