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Galovic V.,Institute of Lowland Forestry and Environment | Rausch T.,Institute of Plant science | Grsic-Rausch S.,SERVA Electrophoresis
Archives of Biological Sciences | Year: 2010

The mature embryos of fourteen elite winter wheat cultivars have been transformed by a biolistic approach. The gene coding for γ-glutamylcysteine synthetase (EC 6.3.2.2) was used as a transgene in order to obtain stable transformants resistant to drought stress. A binary vector, pBinarUTRECS, was used. The gene was under the control of the CaMV35S promoter region. GUS::GFP gene fusion was used as a reporter system and nptII served as a selectable marker gene. A high regeneration capacity of callus tissue under the selective pressure and successful GUS assay of transformed tissue were an indication of successful insertion of a transgene into mature embryo derived wheat tissue.


Vukov D.,University of Novi Sad | Galic Z.,Institute of Lowland Forestry and Environment | Rucando M.,University of Novi Sad | Ilic M.,University of Novi Sad | And 4 more authors.
Archives of Biological Sciences | Year: 2016

This study was carried out on the Vidlic Mountain, eastern Serbia. The herb layer was surveyed in permanent plots on two localities: in a naturally regenerated beech forest and in a Douglas-fir plantation, in spring, summer and autumn 2011, 2012 and 2013. Air temperature, air humidity and soil moisture were measured. Species richness, Shannon's diversity index and Pielou's evenness index were calculated for each plot. Comparison of the abundances of species common to both forest stands was done using the Mann-Whitney U-test. The compositional gradient of the species data was examined using detrended correspondence analysis (DCA), and the species-environment relationship was analyzed by canonical correspondence analysis (CCA). Soil moisture and the total herb cover significantly differed in the naturally regenerated beech forest and Douglas-fir plantation. Floristic similarity between the surveyed forest stands was 28.12%. Although the dominant canopy species is known to be the strongest predictor of the herb layer, the model that includes all of the analyzed environmental factors explains the largest amount of the species variability. The species best fitted to this model are Dryopteris filix-mas, Galium odoratum, Pulmonaria officinalis, Sanicula europaea, Pteridium aquilinum and Rubus caesius. The analyzed forest stands are examples of two different post-disturbance regeneration strategies. Having in mind the limitations of this study, we can conclude that the naturally regenerated beech forest recovers faster: its herbaceous layer indicated nearly natural conditions, with only a few pioneer and disturbance-tolerant species. The herb layer in the Douglas-fir stand is still in the early seral stage, i.e. establishment.


Galovic V.,Institute of lowland forestry and environment | Orlovic S.,Institute of lowland forestry and environment | Pap P.,Institute of lowland forestry and environment | Kovacevic B.,Institute of lowland forestry and environment | Markovic M.,Institute of lowland forestry and environment
Genetika | Year: 2010

Two rust fungi, Melampsora larici populina and Melampsora medusae are common in all poplar growing regions worlwide. M. larici populina is native to Eurasia, while M. medusae is endemic to eastern Unated States on Populus deltoides. Poplar leaf rust (Melampsora sp.) is widely spread disease in our country, and can cause significant growing problems. Race that prevails in our growing region is M. Larici populina. This work initiated the research of population composition, i.e. qualitative participation of Melampsora races in popoluation of black poplars hybrid progenies using molecular methods. When five SSR markers were used the results showed that M. larici populina was found in three tested clones, while only M. medusae was determined in clone I-214.


Stevanov M.,Institute of Lowland Forestry and Environment | Ostoic S.K.,Croatian Forest Research Institute | Vuletic D.,Croatian Forest Research Institute | Orlovic S.,Institute of Lowland Forestry and Environment
Periodicum Biologorum | Year: 2013

Background and purpose: The aimof the paper is to describe and analyse the consultation process on forest biomass and sustainable forestmanagement in the context of renewable energy. Rather unique for the cross-border region of Croatian and Serbia the process was initiated within the EU project RoK-FOR [1] and based on a 'triple helix' principle- taking into consideration perspectives of science, administration and stakeholders [2 in 3]. Achieved body of information provides insights into forest-related issues in the context of renewable energy in the Western Balkans. Materials and methods: The methods and analytical frame used for the purpose of this article was proposed by [3], focusing on: actors and their roles in the process; instruments and tools used in the process of gathering information and consultation; and assessing the level of interaction among actors during the consultation processes. Stakeholders' panel was the main instrument that generated »body of information«, whereas structured interviews were used for better understanding of actors involved. For understanding administrative and legislative framework the whole set of documents and decisions from both countries was analysed while observational notes helped to critically reflect upon data obtained by stakeholder interviews. Results and conclusions: Use of stakeholders' panel as a platform for creating a rather robust »body of information« proved to be valuable tool in the consultation processes,which resulted in a consensus of participating actors on strategic research topics and common recognition ofmajor challenges related to forest biomass production and use. An overall level of interaction among participants can in both countries be classified as of a »medium intensity«, although the ways of interaction were somewhat different. The results could serve as inputs to national research and/or energy strategies in context of achieving renewable energy goals and fulfilment of obligations according to the Kyoto Protocol.


Popovic B.M.,University of Novi Sad | Stajner D.,University of Novi Sad | Zdero-Pavlovic R.,University of Novi Sad | Tumbas-Saponjac V.,University of Novi Sad | And 2 more authors.
Plant Physiology and Biochemistry | Year: 2016

This paper is aimed to characterize young poplar plants under the influence of water stress provoked by polyethileneglycol 6000 (PEG 6000). Three polar genotypes (M1, B229, and PE19/66) were grown in hydroponics and subjected to 100 and 200 mOsm PEG 6000 during six days. Polyphenol characterization, two enzymatic markers and antioxidant capacity in leaves and roots were investigated in stressed plants. Total phenol content, ferric reducing antioxidant capacity (FRAP) and DPPH antiradical power (DPPH ARP) were determined for estimating total antioxidant capacity. Polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were determined as enzymatic markers. Polyphenol characterization of poplar samples was performed by HPLC-PDA analysis. All results were subjected to correlation analysis and principal component analysis (PCA). Inspite of the decrease of total phenol content in investigated genotypes, as well as total antioxidant capacity, some of polyphenols were affected by stress like flavonoids chrysin, myricetine, kaempferol and isoferulic acid in roots of B229 genotype (Populus deltoides). Genotype B229 also showed the increase of antioxidant capacity and PAL activity in root and leaves under stress what could be the indicator of the adaptability of poplar plants to water stress. Significant positive correlations were obtained between PAL, antioxidant capacity as well as phenolic acids among themselves. Chemometric evaluation showed close interdependence between flavonoids, FRAP, DPPH antiradical power and both investigated enzymes of polyphenol metabolism, PAL and PPO. © 2016 Elsevier Masson SAS.


Lorz C.,Sudan University of Science and Technology | Furst C.,Sudan University of Science and Technology | Galic Z.,Institute of Lowland Forestry and Environment | Matijasic D.,Swedish University of Agricultural Sciences | And 6 more authors.
Environmental Management | Year: 2010

We assessed the probability of three major natural hazards-windthrow, drought, and forest fire-for Central and South-Eastern European forests which are major threats for the provision of forest goods and ecosystem services. In addition, we analyzed spatial distribution and implications for a future oriented management of forested landscapes. For estimating the probability of windthrow, we used rooting depth and average wind speed. Probabilities of drought and fire were calculated from climatic and total water balance during growing season. As an approximation to climate change scenarios, we used a simplified approach with a general increase of pET by 20%. Monitoring data from the pan-European forests crown condition program and observed burnt areas and hot spots from the European Forest Fire Information System were used to test the plausibility of probability maps. Regions with high probabilities of natural hazard are identified and management strategies to minimize probability of natural hazards are discussed. We suggest future research should focus on (i) estimating probabilities using process based models (including sensitivity analysis), (ii) defining probability in terms of economic loss, (iii) including biotic hazards, (iv) using more detailed data sets on natural hazards, forest inventories and climate change scenarios, and (v) developing a framework of adaptive risk management. © 2010 Springer Science+Business Media, LLC.


Ballian D.,University of Sarajevo | Bogunic F.,University of Sarajevo | Cabaravdic A.,University of Sarajevo | Pekec S.,Institute of Lowland Forestry and Environment | Franjic J.,University of Zagreb
Periodicum Biologorum | Year: 2012

Background and Purpose: The wild cherry (Prunus avium) has great and multiple importance. The fruits it produces are used for several purposes (as food for people, birds and other animals, as well as in phytotherapy). As many birds and mammals feed on the fruit of the wild cherry, it has the ability of dispersion over large areas in a very short time. It is present in from river deposits up to 1900 m/alt, while it is quite rare in the Submediterranean. Wild cherry grows as a solitary tree or in small groups, usually at the edge of the forest or within the forest in areas with more sunlight. The significance of the wild cherry is reflected in the high economic value of its wood, which makes itmuch demanded and popular, and thus endangered. Materials and Methods: The plant material was collected from 22 natural populations in Bosnia and Herzegovina. The fruit and leaves were collected from marginal or solitary trees, usually from the south-facing, outer sun-exposed parts of the tree crown. We measured the following fruit characteristics: fruit length (FL), fruit width (FW), fruit thickness (FT), seed length (SL), seed width (SW), seed thickness (ST), length of the stalk (LOS) and width of the stalk (WOS), and leaf characteristics: length of the petiole (LP), length of the leaf blade (LB), distance from the blade base to the blade's widest part (BBW), width of the leaf blade (WB), insertion angle of the leaf venation (AV), number of leaf teeth on a 2-cm length (NT), blade width at 1 cmfrom the blade apex (WBA) and blade width at 1 cm from the blade's base (WBB). All statistical analyses of the data were made using the SPSS 15.0 package for Windows. Results: The results obtained show the presence of a high level of intrapopulational, as well as interpopulational, morphological variability in the natural populations of the wild cherry which have been investigated. Analyses of population differentiation have not confirmed our expectations. Our results only indicate differentiation in fruit size characteristics, but the indicators are very weak. The resulting high values of the regression coefficient in this research can serve to estimate the values of some features and characteristics without their measurement. Conclusions: The analyses of 16 morphological characteristics in 22 natural populations of the wild cherry in Bosnia and Herzegovina showed statistically significant differences between investigated populations. Differentiation in natural populations of the wild cherry was very low and identified only in fruit dimension characteristics.


Rakic M.,University of Novi Sad | Karaman M.,University of Novi Sad | Forkapic S.,University of Novi Sad | Hansman J.,University of Novi Sad | And 3 more authors.
Environmental Science and Pollution Research | Year: 2014

Edible and medicinal macrofungi used in human diet represent not only important sources of nutritive elements but toxic substances as well (heavy metals and radionuclides). Radioactivity levels of four radionuclides (40K, 137Cs, 226Ra, 228Ra) were determined in the basidiomata (fruiting bodies of a Basidiomycetes) of six lignicolous (Fomitopsis pinicola, Ganoderma applanatum, Hericium clathroides, Megacollybia platyphylla, Pluteus cervinus, Trametes gibbosa) and three mycorrhizal (Boletus luridus, Boletus sp. 1, Boletus sp. 2) species as well as their soil (wood) substrates by gamma spectrometry (high-resolution high-purity germanium (HPGe) detector). The aim was to investigate their ability for radionuclide absorption according to transfer factors (from soil and wood), to predict potential bioindicator species as well as species with potential risk for human use. Samples were taken during years 2011 and 2012, at two sites in forest ecosystem of Tara Mountain (Serbia). Observed concentration ranges per dry weight were as follows: 29–3,020 Bq/kg (40K), 21.9–735 Bq/kg (137Cs), 3–39 Bq/kg (226Ra), and 2.0–18 Bq/kg (228Ra). Obtained results indicate that the type of basidiome (fleshy/tough), most likely due to a different metabolic rate, has a very important role in radionuclide accumulation. The highest activity concentrations of all analyzed radionuclides were found in species with fleshy basidiomata—P. cervinus, H. clathroides, M. platyphylla, and Boletus species. A species-specific influence on radionuclide uptake was more prominent comparing to habitat differences and the role of fungal trophic mode. No significant variations were observed regarding radionuclide activity among the same fungal species from different sampling sites. © 2014, Springer-Verlag Berlin Heidelberg.


Capelja E.,University of Novi Sad | Stevic N.,University of Novi Sad | Galovic V.,Institute of Lowland Forestry and Environment | Novakovic M.,University of Novi Sad | Karaman M.,University of Novi Sad
Genetika | Year: 2014

Determination of fungal species by traditional morphological approach can often be problematic. In the phylum Basidiomycota, sporocarps of different species can share very similar morphoanatomical characteristics. Using molecular markers and phylogenetic species concept this problem can be reduced. In this study identification of six autochtonous fungal species, collected from several locations in Serbia (Tara, Kopaonik, Stara planina) was done by comparison between morphological and molecular data of fungal species, as well as information obtained from phylogenetic tree. ITS sequences amplified from 11 specimens of two genera of ph. Basidiomycota: Marasmius and Ganoderma, were compared with ITS sequences from database using basic local alignment search tool (BLAST). Phylogenetic tree was constructed using Neighbor joining method based on differences between analyzed ITS sequences. Our results showed that within genera Marasmius and Ganoderma morphological and molecular determinations are usually in accordance, but for proper species delimitation both approaches should be used.


PubMed | University of Novi Sad and Institute of Lowland Forestry and Environment
Type: | Journal: Plant physiology and biochemistry : PPB | Year: 2016

This paper is aimed to characterize young poplar plants under the influence of water stress provoked by polyethileneglycol 6000 (PEG 6000). Three polar genotypes (M1, B229, and PE19/66) were grown in hydroponics and subjected to 100 and 200 mOsm PEG 6000 during six days. Polyphenol characterization, two enzymatic markers and antioxidant capacity in leaves and roots were investigated in stressed plants. Total phenol content, ferric reducing antioxidant capacity (FRAP) and DPPH antiradical power (DPPH ARP) were determined for estimating total antioxidant capacity. Polyphenol oxidase (PPO) and phenylalanine ammonia lyase (PAL) were determined as enzymatic markers. Polyphenol characterization of poplar samples was performed by HPLC-PDA analysis. All results were subjected to correlation analysis and principal component analysis (PCA). Inspite of the decrease of total phenol content in investigated genotypes, as well as total antioxidant capacity, some of polyphenols were affected by stress like flavonoids chrysin, myricetine, kaempferol and isoferulic acid in roots of B229 genotype (Populus deltoides). Genotype B229 also showed the increase of antioxidant capacity and PAL activity in root and leaves under stress what could be the indicator of the adaptability of poplar plants to water stress. Significant positive correlations were obtained between PAL, antioxidant capacity as well as phenolic acids among themselves. Chemometric evaluation showed close interdependence between flavonoids, FRAP, DPPH antiradical power and both investigated enzymes of polyphenol metabolism, PAL and PPO.

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