Pogorelov V.M.,Hematology Research Center |
Ivanova L.A.,Institute of Labor Medicine |
Kozinets G.I.,Hematology Research Center
Gematologiya i Transfusiologiya | Year: 2012
Complete blood count (CBC) and leukocyte differential count (LDC) is the most frequently requested clinical laboratory tests. These analyses are highly automated, and the correct interpretation of results requires extensive knowledge of the analytic performance of the instruments and the clinical significance of the results they provide. We analyze the state of the art regarding traditional and new parameters with emphasis on clinical applications and analytical quality. The problems of some traditional parameters of the CBC count, such as platelet counts, some components of the LDC, such as monocyte and basophil counts, and other commonly used values, for example red cell volume distribution and platelet counts (mean platelet volume and platelet distribution) are discussed. The new parameters, evaluated from analytical and clinical viewpoints, are the available components of the extended differential count (stem cells, immature granulocytes, and erythroblasts), the immature reticulocyte fraction, the reticulocyte counts, the fragmented RBCs, and the immature platelet fraction. The potentialities of the most commonly used automated hematology analyzers are critically analyzed, with special attention to cytometry systems and cell image analyzers. The lecture is intended for special training of clinical laboratory staff and the formation of profound knowledge of laboratory hematology.