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Zhao Q.,Shanghai University of Traditional Chinese Medicine | Cai D.,Institute of Integrative Medicine | Cai D.,Fudan University | Bai Y.,Shanghai University of Traditional Chinese Medicine
International Journal of Molecular Medicine | Year: 2013

The monoamine oxidase type-B (MAO-B) inhibitor, selegiline, is often recommended as a first-line treatment for Parkinson's disease (PD) and has been shwon to possess neuroprotective effects. The aim of the present study was to determine whether selegiline increases the levels of the neurotrophic factors (NTFs), glial cell line-derived neurotrophic factor (GDNF) and brain-derived neurotrophic factor (BDNF), and whether it rescues motor dysfunction and the loss of dopaminergic neurons in mice with 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP)-induced lesions. We found that the oral administration of selegiline (1.0 mg/kg/day for 14 days) successfully suppressed the MPTP-induced reduction of nigral dopaminergic neurons and striatal fibers (192.68 and 162.76% of MPTP-exposed animals, respectively; both P<0.001). Moreover, improvements in gait dysfunction were observed after 7 and 14 days of a low dose of selegiline that is reported not to inhibit MAO-B. Furthermore, there was a significant increase in GDNF and BDNF mRNA (2.10 and 2.75-fold) and protein levels (143.53 and 157.05%) in the selegiline-treated mice compared with the saline-treated MPTP-exposed mice. In addition, the Bax/Bcl-2 gene and protein expression ratios were significantly increased in the MPTP-exposed mice, and this effect was reversed by selegiline. Correlation analysis revealed that gait measurement and GDNF/BDNF levels positively correlated with the number of dopaminergic neurons. These findings demonstrate that selegiline has neurorescue effects that are possibly associated with the induction of NTFs and anti-apoptotic genes.

Jiang H.-W.,Institute of Integrative Medicine | Xue S.-Y.,PLA Fourth Military Medical University
Journal of Xi'an Jiaotong University (Medical Sciences) | Year: 2011

Objective: To observe the protective effects on diabetic nephropathy (DN) rats by Xueshuantong injection combined with benazepril and to explore its therapeutic mechanism. Methods: DN rat models were prepared by high-glucose and high-fat forage added with small-dose STZ. The rats were randomized into DN group without intervention (DN group), DN with benazepril group (B group), DN with benazepril and Xueshuantong injection group (X group) and normal group (N group). The level of h-CRP and TNF-α expression was measured at the end of 12th week. The renal pathomorphologic changes were observed. Results: The level of h-CRP and TNF-α expression in X group obviously decreased as compared with that in DN group (P<0.01). The level of h-CRP and TNF-2 expression in X group significantly decrensed as comparerd with that in B group. Gromeralor basement membrane thickening and mesangial cell proliferative lesions were significantly improved. Conclusion: Xueshuantong injection combined with benazepril can significantly protect DN rat's kidney, which might be its mechanism of reducing h-CRP and TNF-α expression and inhibiting the DN proliferation of mesangial matrix and deposition of extracellular matrix.

Limeira A.B.,Federal University of Pernambuco | Aguiar C.M.,Federal University of Pernambuco | De Lima Bezerra N.S.,Federal University of Pernambuco | De Lima Bezerra N.S.,Institute of Integrative Medicine | Camara A.C.,State University of Paraiba
European Journal of Pediatrics | Year: 2013

The aims of the present study were to evaluate the association between breastfeeding and breathing development and to investigate associations between breastfeeding duration and the breathing patterns in children. A crosssectional study was carried out at the Institute of Integrative Medicine Professor Fernando Figueira, Recife, Brazil, with a random sample of 732 children aged between 6 and 9 years. Breastfeeding and breathing patterns were identified using a questionnaire that was filled out by mothers or guardians, and a clinical examination of the children. Data were analyzed statistically by Pearson's chi-square test at 5 % significance level. The prevalence of mouth breathing was 48 %, whereas 52 % of the children were nasal breathers. Six hundred-forty children were breastfed; 46.2 % of them were mouth breathers and 53.8 % were nasal breathers. Ninety-two children were not breastfed; 59.8 % of them were mouth breathers and 40.2 % were nasal breathers. Breastfeeding for 24 months or more, as well as exclusive breastfeeding in the first 6 months, was associated with the development of nasal breathing. Conclusions Extended breastfeeding was associated with correct development of the breathing pattern. © Springer-Verlag Berlin Heidelberg 2012.

Ciaramella A.,Institute of Integrative Medicine | Poli P.,Pain Therapy Unit
Journal of Nervous and Mental Disease | Year: 2015

This retrospective study investigated the influence of psychiatric comorbidity on pain perception and coping with pain in tertiary pain clinic patients, 427 treated for chronic low back pain (CLBP) and 629 for other forms of chronic pain (CG). No differences in psychosomatic dimensions were found between the two groups, but Italian Pain Questionnaire dimensions and intensity scores (t = 7.35; p < 0.0001) were higher in CLBP than in CG subjects. According to the Mini-International Neuropsychiatric Interview, CLBP patients also had a higher prevalence of lifetime major depressive episodes (χ2 = 4.96; p < 0.05), dysthymic disorder (χ2 = 4.64; p < 0.05), suicide risk (χ2 = 10.43; p < 0.01), and agoraphobia (χ2 = 6.31; p < 0.05) than CG patients did. The Multidimensional Pain Inventory showed a close association between CLBP and both agoraphobia (χ2 = 3.74; p < 0.05) and dysfunctional coping style (χ2 = 8.25; p < 0.01), which increased disability. Both agoraphobia and lifetime depression were associated with an overall increase in dimensions and pain intensity in CLBP, but not in CG. © 2015 Wolters Kluwer Health, Inc.

Choudhary S.P.,Jammu University | Bhardwaj R.,Guru Nanak Dev University | Gupta B.D.,Institute of Integrative Medicine | Dutt P.,Institute of Integrative Medicine | And 3 more authors.
Physiologia Plantarum | Year: 2010

In the present study, the effects of epibrassinolide (EBL) on indole-3-acetic acid (IAA), abscisic acid (ABA) and polyamine (PA) tissue concentrations and antioxidant potential of 7-day-old Raphanus sativus L. cv. 'Pusa chetki' seedlings grown under Cu stress were investigated. EBL treatment alone or in combination with Cu enhanced free and bound IAA titers when compared with the metal alone. Modest increases in free and bound ABA contents were observed for EBL treatment alone. However, the combination of EBL with Cu caused major increases in both forms of ABA, over Cu alone. Among the PAs analyzed, only putrescine and cadaverine concentrations were enhanced by EBL treatment alone. By contrast, a significant decline in putrescine and spermine contents was found in seedlings treated with EBL plus Cu. EBL treatments alone or in combination with Cu enhanced activities of guaiacol peroxidase (EC1.11.1.7), catalase (EC, superoxide dismutase (EC and glutathione reductase (EC and protein contents in comparison with metal and control treatments. A major decrease in malondialdehyde content was also recorded for EBL treatments with or without Cu. An increase in phytochelatin content was also observed in seedlings treated with EBL alone or in combination with Cu. Major improvement in radical scavenging activities, as attested by the antioxidant activity assay using DPPH (1,1-diphenylpicrylhydrazyl), and elevated deoxyribose and reducing powers, along with increased contents of ascorbic acid, total phenols and proline, also suggest a major influence of EBL application in mitigating copper-induced oxidative stress in radish seedlings. © Physiologia Plantarum 2010.

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