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Krumina G.,Institute of Innovative Biomedical Technology | Babarykin D.,Institute of Innovative Biomedical Technology | Krumina Z.,Institute of Innovative Biomedical Technology | Paegle I.,Laboratory of Pathohistology | And 5 more authors.
Journal of Trauma and Acute Care Surgery | Year: 2013

Background: The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells (BM MMSCs) on recovery after polytrauma and bone fracture repair. Methods: A total 27 Wistar-Kyoto rats were divided into three groups (n = 9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSC transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours 9 days after surgery, nine rats received allogeneic BM MMSCs (1 × 106 cells per kilogram) intravenously. The day before operation and at Days 3 and 10 after surgery as well as at the end of the experiment, blood analysis was carried out. At 10, 20, and 30 days after surgery the rats' locomotor activity was assessed in an open-field test. At Day 30, rats were euthanized, and macroscopic and histologic observations of rats' lower extremities was performed. Results: The treated animals gained weight faster regained their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios, as well as less marked muscle atrophy. Rat treatment with BM MMSC transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood cell analysis results showed delayed recovery after hemorrhage in the rats receiving allogeneic BM MMSCs: restoration of red blood cell counts, hematocrit level, and hemoglobin level was slower in the untreated animals. Conclusion: Allogeneic BM MMSC transplantation improved rats rehabilitation scores after experimental polytrauma. Copyright © 2013 by Lippincott Williams & Wilkins. Source


Krumina G.,Institute of Innovative Biomedical Technology | Ratkevicha L.,University of Latvia | Vizma V.N.,University of Latvia | Babarikina A.,Institute of Innovative Biomedical Technology | Babarykin D.,Institute of Innovative Biomedical Technology
Proceedings of the Estonian Academy of Sciences | Year: 2015

The objective of this study was to investigative the effects of ten plant extracts, six juices, and propolis and their combinations on the in vitro growth of oral pathogens Streptococcus mutans and Candida albicans. Agar-well diffusion and broth dilution methods were used. Triple and quadruple combinations were tested with the most active extracts. All of the tested 70% ethanolic extracts inhibited the growth of S. mutans and C. albicans. Cloves, cinnamon, propolis, lavender, and sage were the most active inhibitors. Apple, black chokeberry, black elderberry, cranberry, Japanese quince, and lemon juice demonstrated little activity. Mixed in double 1: 1 combinations, 8 extract combinations expressed synergistic action and 11 combinations expressed antagonistic action to the inhibition of the growth of C. albicans. Chamomile, liquorice, marigold, and lavender were involved both in synergistic and antagonistic interactions depending on the second component of the mixture. Propolis, cinnamon, and cloves were involved only in particular synergistic interactions, while sweet flag, dog rose, and oregano related only to some antagonistic interactions. The most active quadruple combination consisted of cloves, cinnamon, propolis, and lavender. Moreover, it demonstrated activity and synergistic action against both microorganisms. Propolis and all the studied plant extracts may be of great interest for inhibiting the growth of oral pathogens S. mutans and C. albicans. © 2015, Estonian Academy Publishers. All rights reserved. Source


Krumina G.,Institute of Innovative Biomedical Technology | Babarykin D.,Institute of Innovative Biomedical Technology | Krumina Z.,Institute of Innovative Biomedical Technology | Paegle I.,Riga Hospital of Traumatology and Orthopedics | And 4 more authors.
IFMBE Proceedings | Year: 2013

The aim of this study was to evaluate impact of transplantation of bone marrow mesenchymal stromal cells on recovery after polytrauma and bone fracture repair. 27 Wistar-Kyoto rats were divided into 3 groups (n=9): normal control (A), polytrauma (B), and polytrauma treated with BM MMSCs transplantation (C). The experimental polytrauma model was made on male rats by causing multiple fractures and hemorrhagic shock. At 36 hours and nine days after surgery 9 rats received syngeneic BM MMSCs (1×106 cells/kg) intravenously. In 30 days post-surgery period changes in animals' body temperature, weight and locomotor behavior as well as blood parameters were recorded. At day 30 rats were euthanized and macroscopic and histological observations of rats lower extremities was performed. The treated animals showed faster weight gain, as well as regaining their physical activity earlier. These outcomes were associated with locomotor activity test results, blood glucose and lactate ratios and less marked muscle atrophy. Rat treatment with BM MMSCs transplantation stimulated bone fracture healing - bone edge consolidation and enhanced callus formation, as well as the size and maturity of newly formed trabeculae. Red blood analysis results showed delayed recovery after hemorrhage in the rats receiving BM MMSCs: restoration of RBC counts, hematocrit and HGB level velocity was less than in the untreated animals. BM MMSCs transplantation improved rats rehabilitation scores after experimental polytrauma. © 2013 IFMBE. Source

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