Institute of Immunology and Microbiology

Prague, Czech Republic

Institute of Immunology and Microbiology

Prague, Czech Republic
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Lyu S.-M.,Guangdong University of Technology | Yu Q.,Guangdong Medical University | Si-Tu Y.-L.,Institute of Immunology and Microbiology | Sun J.-Y.,Guangdong Medical University | And 3 more authors.
Chinese Pharmacological Bulletin | Year: 2016

Aim 'lo investigate the effects of red yeast rice capsules containing coenzyme Ql0 on femur with an animal model of osteoporosis, which was induced by OVX with D-galactose in rats, and the results were compared with those obtained from diethylstilbestrol. Methods Three-month old female Sprague-Dawley rats were randomly divided into sham group) (CON), ovariectomized group ( OVX ), model group ( MOD), diethylstilbestrol group(L)KS), and red yeast rice capsule group(KYH). After 60 days, the left femurs were collected for Ca, P and hyp measurement, while the right femurs were performed with three-point bending test and miero-CT evaluation, respectively. Results Compared with CON group, MOD group had a significant increase in body weight, Tb. Sp, SMI and significant decrease in maximum load, stiffness, maximum strength, break strength, elastic modulus, Ca, P, Hyp contents and indicators of BV/TV, Tb. N, BMD, Conn-Dens. On the other hand, compared with MOD group, RYR group had a lower body weight and all bone biomechanics indexes were increased without statistically significant difference. At the same time, the content of Ca, P and indicators of BV/TV, Tb. N, Th. Th, BMD, Conn-Dens increased significantly; yet Tb. Sp decreased significantly. In DES group, the results of indicators were consistent with those for RYR group. In addition, compared with DES group, in RYR group body weight decreased significantly; the content of Ca, P and indicators of BV/TV, Tb. N, Tb. Th, Conn- Dens were significantly higher, and Tb. Sp, SMI were significantly lower. Conclusions Significant bone loss and deteriorated mechanical properties of femur can be observed in animal model of osteoporosis induced by OVX combined with D-galactose. Red yeast rice capsules containing coenzyme Q10 show effective prevention effects. Furthermore, red yeast rice capsules(0.5 tablet ? kg-1) have better effect on increasing the number of trabecular bone than diethylstilbestrol (30 μg • kg-1) does.


Jindrova Z.,Institute of Immunology and Microbiology | Jindrova Z.,Charles University | Hanusova Z.,Institute of Immunology and Microbiology | Holada K.,Institute of Immunology and Microbiology
Chemicke Listy | Year: 2015

Protease-activated receptors (PARs) are transmembrane proteins which rank among G-protein-coupled receptors. So far, four PARs (PAR1-4) have been described. They are activated by a protease cleavage at the N-terminal part of the receptor. Through the cleavage a new N-terminus appears which acts as a ligand activating the receptor. A peptide of the same amino acid sequence as the new N-terminus can activate the receptor without its cleavage. Some non-specific proteases can cleave PAR receptors at different sites, which results in changes in cell signaling. Higher activities of PARs have been observed under various pathological conditions, such as thrombosis, atherosclerosis, inflammations or neurodegeneration. Specific modulators of PAR signaling are a promising class of compounds with a wide therapeutic potential. First PAR inhibitors were based mainly on the amino acid sequence in the activating peptides. Recently, new, low-molecularweight, very specific and effective inhibitors have been developed. One of them, vorapaxar, passed the clinical tests and was introduced to the market. © 2015, Czech Society of Chemical Engineering. All rights reserved.

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