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Biskup I.,Wroclaw Medical University | Golonka I.,Wroclaw Medical University | Gamian A.,Institute of Immunology and Experimental Therapy | Gamian A.,Wroclaw Medical University | Sroka Z.,Wroclaw Medical University
Postepy Higieny i Medycyny Doswiadczalnej | Year: 2013

Introduction: Phenols are the most abundant compounds in nature. They are strong antioxidants. Too high level of free radicals leads to cell and tissue damage, which may cause asthma, Alzheimer disease, cancers, etc. Taking phenolics with the diet as supplements or natural medicines is important for homeostasis of the organism. Materials and methods: The ten most popular water soluble phenols were chosen for the experiment to investigate their antioxidant properties using ABTS radical scavenging capacity assay and ferric reducing antioxidant potential (FRAP) assay. Results and discussion: Antioxidant properties of selected phenols in the ABTS test expressed as IC50 ranged from 4.332 μM to 852.713 μM (for gallic acid and 4-hydroxyphenylacetic acid respectively). Antioxidant properties in the FRAP test are expressed as μmol Fe2+/ml. All examined phenols reduced ferric ions at concentration 1.00 x 10-3 mg/ml. Both methods are very useful for determination of antioxidant capacity of water soluble phenols. Source

Jakimowicz D.,Wroclaw University | Jakimowicz D.,Institute of Immunology and Experimental Therapy | Van Wezel G.P.,Leiden University
Molecular Microbiology | Year: 2012

Streptomycetes are antibiotic-producing filamentous microorganisms that have a mycelial life style. In many ways streptomycetes are the odd ones out in terms of cell division. While the basic components of the cell division machinery are similar to those found in rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, many aspects of the control of cell division and its co-ordination with chromosome segregation are remarkably different. The rather astonishing fact that cell division is not essential for growth makes these bacteria unique. The fundamental difference between the cross-walls produced during normal growth and sporulation septa formed in aerial hyphae, and the role of the divisome in their formation are discussed. We then take a closer look at the way septum site localization is regulated in the long and multinucleoid Streptomyces hyphae, with particular focus on actinomycete-specific proteins and the role of nucleoid segregation and condensation. © 2012 Blackwell Publishing Ltd. Source

Kasprzyk A.,Wroclaw Medical University | Zbikowska B.,Wroclaw Medical University | Sroka Z.,Wroclaw Medical University | Gamian A.,Institute of Immunology and Experimental Therapy | Gamian A.,Wroclaw Medical University
Postepy Higieny i Medycyny Doswiadczalnej | Year: 2012

Introduction: Free radicals and reactive oxygen species are compounds usually present in healthy organisms as natural products of many metabolic pathways, and they are important in cell signaling and homeostasis. As a source of reactive oxygen species one can mention phagocytic cells and enzymes such as xanthine oxidase. Sometimes the level of reactive oxygen species strongly increases. This may lead to damage of very important cell structures such as nucleic acids, proteins or lipids. In this situation one should provide the organism with powerful antioxidants as a medicine or in the diet. A rich source of strong antioxidants such as phenolic compounds is plant raw materials, which are the subject of our study. Material/Methods: Antiradical potential of extracts was measured with DPPH radical (2,2-diphenyl-1-picrylhydrazyl) and was expressed as the number of units per mg of extracts (TAU 515/mg) and per g of raw material (TAU 515/g). The amount of phenolic compounds was determined colorimetrically using Folin-Ciocalteu phenol reagent (3H 2O · P 2O 5 · 13WO 3 · 5MoO 3 · 10H 2O). Results: The strongest antiradical activity was noted for extracts obtained from Cinnamomi cortex; the number of antiradical units per mg of extract (TAU 515/mg) was 10.31±1.052. The lowest antiradical features were exhibited by extract from Zingiberis rhizoma (0.28±0.174) and extract from Cichorii radix (0.38±0.669). The highest amount of phenolic compounds was measured for extracts from Bistortae rhizoma, with a value (in percentage) of 78.6±13.5. The correlation coefficient between the number of antiradical units in extracts and amount of phenolic compounds in these extracts was 0.7273. When the number of antiradical units was calculated per g of raw material (TAU 515/g) the strongest antiradical properties were noted for Bistortae rhizoma (1406±274.9), the weakest for Cichorii radix (122±158.3). Source

Wickiewicz D.,Wroclaw Medical University | Chrobak A.,Institute of Immunology and Experimental Therapy | Chrobak A.,Wroclaw University | Gmyrek G.B.,University of Washington | And 5 more authors.
Archives of Gynecology and Obstetrics | Year: 2013

Purpose: To determine, with extended receiver operating characteristic (ROC) curve analysis, the diagnostic value of cytokines showing significantly different peritoneal concentrations between women with and without endometriosis. Methods: Multiplex cytokine concentration measurement of IL-2, IL-4, IL-6, IL-10, TNF-α and IFN-γ levels in peritoneal fluid of women with minimal to mild (n = 10) and moderate to severe (n = 26) endometriosis, and 42 controls. Results: Only IL-6 and IL-10 concentrations were significantly higher in endometriosis patients than in controls. Specifically, significantly higher IL-6 and IL-10 levels were found in moderate to severe but not in minimal to mild endometriosis as compared to controls. For evaluation of diagnostic significance, ROC analysis determined discriminating parameters for IL-6, while those calculated for IL-10 were useless. Importantly, ROC analysis for IL-6 levels limited to women with moderate to severe endometriosis showed the highest area under the curve with the sample size sufficient to achieve 90 % power of the test. Finally, extended ROC including cost of analysis for this group of patients determined the optimal cut-off leading to high specificity and positive likelihood ratio resulting in 79 % effectiveness of the test. Conclusions: While our outcomes show moderate usefulness of peritoneal IL-6 levels in discrimination of moderate to severe endometriosis, further studies might be needed to determine the usefulness of peritoneal IL-6 levels in detection of early stages of endometriosis, as such a finding would be more relevant in clinical decision making. © 2013 Springer-Verlag Berlin Heidelberg. Source

Lukawska M.,Institute of Biotechnology and Antibiotics | Wietrzyk J.,Institute of Immunology and Experimental Therapy | Opolski A.,Institute of Immunology and Experimental Therapy | Oszczapowicz J.,University of Warsaw | Oszczapowicz I.,Institute of Biotechnology and Antibiotics
Investigational New Drugs | Year: 2010

Summary: Oxazolinodaunorubicin, a new daunorubicin derivative with a modified daunosamine moiety, was synthesized. The biological properties of this derivative and the parent daunorubicin were compared. The results showed antiproliferative activity of the derivative with significantly lower toxicity (an LD50 value ca. 20 times higher than that of parent daunorubicin) and an ability to completely overcome the resistance of cancer cells to this drug in vitro. Cardiotoxicity determination using male mice treated with a single dose of 75% of the LD50 value indicated that the cardiotoxicity of new analog was much lower than that of the parent drug. Preliminary results in transplanted murine tumor models revealed that a single-dose injection of the tested compounds exhibited antitumor activity in P388 and L1210 leukemia and 16/C mammary adenocarcinoma bearing mice. © 2009 Springer Science+Business Media, LLC. Source

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