Institute of Hygienic and Environmental Medicine

Tianjin, China

Institute of Hygienic and Environmental Medicine

Tianjin, China

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Zhou H.,Institute of Hygienic and Environmental Medicine | Zhou H.,Tianjin Key Laboratory of Risk Assessment | Gao Z.,Institute of Hygienic and Environmental Medicine | Gao Z.,Tianjin Key Laboratory of Risk Assessment | And 8 more authors.
Analytical Letters | Year: 2010

A piezoelectric crystal biosensor method has been established for detection of listeria monocytogenes (LM) by gold nanoparticles (GNPs) signal amplification. Based on specific recognition of single-stranded DNA (ssDNA) hybridization, a sandwich format of ssDNA probes/complimentary ssDNA probes modified by GNPs/ssDNA of LM was fabricated. This novel strategy allows the detection limit of LM as low as 10 cfu/mL. The biosensor showed friendly biocompatibility, good specificity, acceptable stability, which provided a potential alternative tool for detection of LM in real samples. Furthermore, the developed method could be easily extended to other gene analysis schemes. © Taylor & Francis Group, LLC.

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