München, Germany
München, Germany

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Rieger M.,Institute of Hydrochemistry | Schaumann G.E.,University of Koblenz-Landau | Mouvenchery Y.K.,University of Koblenz-Landau | Niessner R.,Institute of Hydrochemistry | And 2 more authors.
Analytical and Bioanalytical Chemistry | Year: 2012

Biogeochemical interfaces in soil are dynamic in the spatial and temporal domain and require advanced visualisation and quantification tools to link in vitro experiments with natural systems. This study presents the development, characterization and application of functional nanoparticles coated with monoclonal antibodies to visualise the distribution of benzo[a]pyrene in porous media using magnetic resonance imaging. The labelled particles are 450nm in diameter and interact with benzo[a]pyrene covalently bound to silanized silica gel. They did not bind to benzo[a]pyrene adsorbed to plain silica gel. Although unspecific filtration was low, washing steps are required for visualisation. The ability to visualise benzo[a]pyrene is inversely correlated to the heterogeneity of the soil materials. There are access restrictions to narrow pore spaces which allow the visualisation of only those pathways which are also accessible to bacteria and hydrocolloids. The production of the particles is applicable to other antibodies which extends the range of potential target contaminants. © Springer-Verlag 2012.


Nikanorov A.M.,Russian Academy of Sciences | Bryzgalo V.A.,Institute of Hydrochemistry | Kosmenko L.S.,Institute of Hydrochemistry | Reshetnyak O.S.,Institute of Hydrochemistry
Water Resources | Year: 2011

Variations in the hydrological-hydrochemical state of the aqueous medium in Lena mouth area. The hydrological regime of the river and variations in qualitative water composition in the mouth area are described in detail. Anthropogenic load is estimated in terms of the inflow and the modulus of inflow of pollutants. © 2011 Pleiades Publishing, Ltd.

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